LOCUS       sequence-332486-        1197 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Tom20-CR, an anchor protein of iCMM/NiCMM, in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Miyamoto T, Uosaki H, Mizunoe Y, Han SI, Goto S, Yamanaka D, Masuda 
            M, Yoneyama Y, Nakamura H, Hattori N, Takeuchi Y, Ohno H, Sekiya M, 
            Matsuzaka T, Hakuno F, Takahashi SI, Yahagi N, Ito K, Shimano H
  TITLE     Rapid manipulation of mitochondrial morphology in a living cell with
            iCMM.
  JOURNAL   Cell Rep Methods. 2021 Jul 22;1(4):100052. doi: 
            10.1016/j.crmeth.2021.100052. eCollection 2021 Aug 23.
  PUBMED    35475143
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2021-07-22"; volume: "1(4):100052. doi"; pages: " 
            10.1016/j.crmeth.2021.100052. eCollection 2021 Aug 23"
COMMENT     Sequence Label: Tom20-CR
FEATURES             Location/Qualifiers
     source          1..1197
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(133..135,136..138,139..849)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(178..199)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(439..458)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     786..807
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             862..1140
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 atggtgggtc ggaacagcgc catcgccgcg ggcgtgtgcg gtgccctctt catagggtac
       61 tgcatctact ttgaccgcaa aagacgaagt gaccccaact tcggatccag tgctggtggt
      121 agtgctggtg gtatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      181 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      241 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      301 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
      361 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      421 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      481 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      541 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
      601 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc
      661 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      721 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
      781 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
      841 ctgtacaagg gagcaagtgg aatcctctgg catgagatgt ggcatgaagg cctggaagag
      901 gcatctcgtt tgtactttgg ggaaaggaac gtgaaaggca tgtttgaggt gctggagccc
      961 ttgcatgcta tgatggaacg gggcccccag actctgaagg aaacatcctt taatcaggcc
     1021 tatggtcgag atttaatgga ggcccaagag tggtgcagga agtacatgaa atcagggaat
     1081 gtcaaggacc tcctccaagc ctgggacctc tattatcatg tgttccgacg aatctcaaag
     1141 agtgctggtg gtagtgctgg tggtagtgct ggtggtactg gtggtcctcg agcttaa
//
