LOCUS       sequence-332575-        4803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the synthetic gene BLADE FP6 under the constitutive 
            J23101** promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Romano E, Baumschlager A, Akmeric EB, Palanisamy N, Houmani M, 
            Schmidt G, Ozturk MA, Ernst L, Khammash M, Di Ventura B
  TITLE     Engineering AraC to make it responsive to light instead of 
            arabinose.
  JOURNAL   Nat Chem Biol. 2021 Apr 26. pii: 10.1038/s41589-021-00787-6. doi: 
            10.1038/s41589-021-00787-6.
  PUBMED    33903769
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2021 Apr 26. pii: 10.1038/s41589-021-00787-6. doi: 
            10.1038/s41589-021-00787-6."
COMMENT     Sequence Label: pBLADE_ONLY_A
FEATURES             Location/Qualifiers
     source          1..4803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      961..988
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      2104..2131
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2150..2241
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2242..2310,2311..3102)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2460..2479)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3273..3861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3762..3781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4015..4032
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4047..4187
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4113..4132)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4273..4295
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        4373..4450
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             join(4451..4803,1..730)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(4470..4489)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc
       61 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag
      121 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg
      181 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct
      241 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg
      301 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt
      361 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac
      421 cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga
      481 cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca
      541 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
      601 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc
      661 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      721 cgggcagtga gcgcaacgca attaatgtaa gttagcgcga attgatctgg tttgacagct
      781 tatcatcgac tgcacggtgc accaatgctt ctggcgtcag gcagccatcg gaagctgtgg
      841 tatggctgtg caggtcgtaa atcactgcat aattcgtgtc gctcaaggcg cactcccgtt
      901 ctggataatg ttttttgcgc cgacatcata acggttctgg caaatattct gaaatgagct
      961 agaaggccat cctgacggat ggccttttcg ttggtaacga atcagacaat tgacggcttg
     1021 acggagtagc atagggtttg cagaatccct gcttcgtcca tttgacaggc acattatgca
     1081 tttctagagt ttacactagc tcagtcctag gtatatgcta gctactagag actagagaag
     1141 gagatatacc atgcatacgc tctacgctcc cggcggttat gacattatgg gctatctgat
     1201 tcagattatg aagaggccaa acccccaagt agaactggga cctgttgaca cgtcagttgc
     1261 tctgattctg tgtgacctga agcaaaaaga cacgccaatt gtgtacgcct cggaagcttt
     1321 tctctatatg acaggataca gcaatgcgga ggtcttgggg agaaactgcc gttttcttca
     1381 gtcacccgac ggaatggtca agccgaaatc gacaaggaag tacgtcgact ccaacacgat
     1441 caatacgatg aggaaagcga ttgataggaa cgccgaggtg caggttgagg tggtcaattt
     1501 taagaagaac ggccaacggt ttgtcaactt cttgacgatg attccggtgc gagatgaaac
     1561 aggggaatac cggtacagca tgggtttcca gtgcgaaacg gaaaacgagt cgctccatcc
     1621 accgatggat aatcgggtac gcgaggcttg tcagtacatc agcgatcacc tggcagacag
     1681 caattttgat atcgccagcg tcgcacagca tgtttgcttg tcgccgtcgc gtctgtcaca
     1741 tcttttccgc cagcagttag ggattagcgt cttaagctgg cgcgaggacc aacgtatcag
     1801 ccaggcgaag ctgcttttga gcaccacccg gatgcctatc gccaccgtcg gtcgcaatgt
     1861 tggttttgac gatcaactct atttctcgcg ggtatttaaa aaatgcaccg gggccagccc
     1921 gagcgagttc cgtgccggtt gtgaagaaaa agtgaatgat gtagccgtca agttgtcata
     1981 aatcgatcaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga
     2041 agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta
     2101 agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt ttgtttattt
     2161 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2221 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2281 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2341 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2401 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2461 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     2521 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2581 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2641 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2701 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2761 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2821 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2881 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2941 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     3001 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     3061 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3121 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3181 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3241 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3301 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3361 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     3421 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3481 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3541 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3601 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3661 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3721 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3781 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3841 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3901 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3961 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4021 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     4081 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     4141 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     4201 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     4261 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     4321 cgaggcagca gatcaattcg cgcgcgaagg cgaagcggca tgcatttacg ttgacaccat
     4381 cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag
     4441 ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
     4501 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
     4561 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
     4621 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
     4681 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
     4741 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
     4801 acg
//
