LOCUS       sequence-33259-e         577 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pQCXIH-Flag Smad3 WT
FEATURES             Location/Qualifiers
     source          1..577
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..70
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aacggacctg caggaattga tccgcggccg caccggtgtg accatggact acaaggacga
       61 cgatgacaag tcgtccatcc tgcctttcac tcccccgatc gtgaagcgcc tgctgggctg
      121 gaagaagggc gagcagaacg ggcaggagga gaaatggtgc gagaaggcgg tcaagagcct
      181 ggtcaagaaa ctcaagaaga cggggcagct ggacgagctg gagaaggcca tcaccacgca
      241 gaacgtcaac accaagtgca tcaccatccc caggtccctg gatggccggt tgcaggtgtc
      301 ccatcggaag gggctccctc atgtcatcta ctgccgcctg tggcgatggc cagacctgca
      361 cagccaccac gagctgcggg ccatggagct gtgtgagttc gccttcaata tgaagaagga
      421 cgaggtgtgc gtcaatccct accactacca gagagtagag acaccagttc tacctcctgt
      481 gttggtgcca cgccacacag agatcccggc cgagttcccc ccactggacg actacagcca
      541 ttccatcccc gaaaacacta acttccccgc aggcatc
//
