LOCUS       sequence-33265-e         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCI HA NEDD4L S382A
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          96..228
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     211..230
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        273..291
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             312..338
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tagtttatca cagttaaatt gctaacgcag tcagtgcttc tgacacaaca gtctcgaact
       61 taagctgcag aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg
      121 tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat
      181 aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc
      241 agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag gctagcctcg
      301 agaccatgga ttatccgtat gatgttcctg attatgcaga attcacgcgt ggtaccccgg
      361 gtgggtggct gcgcagggcc ctacctgggc gggagcggct gcagagccct gtccacgcgg
      421 tgcctcccca gcacggcacc tcccactcca ggctgttggt tacctggccg ggagctggca
      481 gagaccagga tttctcctcg ccgccgttgc tgttgttagg ggaaacagac catctgcatc
      541 tagacctgcc tctctctccg ctccccactt cagacgagct cttcctgcct ggaatctgtg
      601 atccgtatgt gaaactttca ttgtacgtag cggatgagaa tagagaactt gctttggtcc
      661 agacaaaaac aattaaaaag acactgaacc c
//
