LOCUS       sequence-33279-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of humanized ChR2(H134R) fused to EYFP driven by 
            CaMKIIa promoter for optogenetic activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Lee JH, Durand R, Gradinaru V, Zhang F, Goshen I, Kim DS, Fenno LE, 
            Ramakrishnan C, Deisseroth K
  TITLE     Global and local fMRI signals driven by neurons defined 
            optogenetically by type and wiring.
  JOURNAL   Nature. 2010 Jun 10. 465(7299):788-92.
  PUBMED    20473285
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jun 10. 465(7299):788-92."
COMMENT     Sequence Label: pAAV-CaMKIIa-hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      428..437
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcgttgctca ggccggtgag gttgctcagg cggataagga tgacaggaca agtgagcagc
       61 cactctgcat agcgcagcca ctgcacccgg tgtcctgtgg caaggtagag catagaggga
      121 ttcttaaact caaaaaagaa ctcgagaatc accttaacca tttcaatggc gcacacatag
      181 atctcctccc agccgcatgt agatttccag gtttggtagg catagaacat cagcagcaaa
      241 atgctgaatc ctgctgcaag ccactgcagg acatttgacg cggtctgagc gccgttcgtg
      301 ccgcgagatt caatccatcc ggcacagtaa cattgatcct cagggaccag gacggacccg
      361 ttcaccacca caggattagt aacgaacaaa agttcgcgtc cgacggcaga caaagcgccg
      421 ccatagtcca tggtggctcc ggagtcgact ctagaggatc cggtaccgct ctagagctgc
      481 ccccagaact aggggccact cgcctgcccg tgctcctgag tgcaaacgga gaaccggctt
      541 gactgacgag cttggggctt ctgagcaggg cactgtggct gctcacagcc tcgcgagcct
      601 tcgtcagcat ccaggtcccc atggcaacca cctccgaaac gccctgttcc cgttgccccg
      661 gtaactgcct ccccaacacc tgcctgcctt tcactttaaa acctgctcct ctttgccctg
      721 gccttatata cacaatactg caggactgtg tgggcccagg agcaagtgga ccctgttccc
      781 ccagggtgat gtactgaggg gggtt
//
