LOCUS       sequence-332829-       14008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14008)
  AUTHORS   Woo MS, Ohta Y, Rabinovitz I, Stossel TP, Blenis J
  TITLE     Ribosomal S6 kinase (RSK) regulates phosphorylation of filamin A on 
            an important regulatory site.
  JOURNAL   Mol Cell Biol. 2004 Apr . 24(7):3025-35.
  PUBMED    15024089
REFERENCE   2  (bases 1 to 14008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2004 Apr . 24(7):3025-35."
COMMENT     Sequence Label: pcDNA3-myc-FLNa S2152A
FEATURES             Location/Qualifiers
     source          1..14008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             904..933
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    9143..9277
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9420..9442)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        complement(9561..9579)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9562..9579)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9599..9616)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9605..9829
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9875..10303
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9962..9981)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10172..10193
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10312..10332)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10317..10646
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10497..10632
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10559..10578
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             10713..11507
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10767..10786)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11377..11396
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11681..11802
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11851..11867)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11851..11867)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11864..11886)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11875..11891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11899..11905,11906..11923,11924..11929))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11944..11965
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12082..12099)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12253..12841)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12333..12352)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13012..13803,13804..13872))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13635..13654
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13873..13977)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 atggagcaga agctgatcag cgaggaggac ctggaattca gtagctccca ctctcgggcg
      961 ggccagagcg cagcaggcgc ggctccgggc ggcggcgtcg acacgcggga cgccgagatg
     1021 ccggccaccg agaaggacct ggcggaggac gcgccatgga agaagatcca gcagaacact
     1081 ttcacgcgct ggtgcaacga gcacctgaag tgcgtgagca agcgcatcgc caacctgcag
     1141 acggacctga gcgacgggct gcggcttatc gcgctgttgg aggtgctcag ccagaagaag
     1201 atgcaccgca agcacaacca gcggcccact ttccgccaaa tgcagcttga gaacgtgtcg
     1261 gtggcactcg agttcctgga ccgcgagagc atcaaactgg tgtccatcga cagcaaggcc
     1321 atcgtggacg ggaacctgaa gctgatcctg ggcctcatct ggaccctgat cctgcactac
     1381 tccatctcca tgcccatgtg ggacgaggag gaggatgagg aggccaagaa gcagaccccc
     1441 aagcagaggc tcctgggctg gatccagaac aagctgccgc agctgcccat caccaacttc
     1501 agccgggact ggcagagcgg ccgggccctg ggcgccctgg tggacagctg tgccccgggc
     1561 ctgtgtcctg actgggactc ttgggacgcc agcaagcccg ttaccaatgc gcgagaggcc
     1621 atgcagcagg cggatgactg gctgggcatc ccccaggtga tcacccccga ggagattgtg
     1681 gaccccaacg tggacgagca ctctgtcatg acctacctgt cccagttccc caaggccaag
     1741 ctgaagccag gggctccctt gcggcccaaa ctgaacccga agaaagcccg tgcctacggg
     1801 ccaggcatcg agcccacagg caacatggtg aagaagcggg cagagttcac tgtggagacc
     1861 agaagtgctg gccagggaga ggtgctggtg tacgtggagg acccggccgg acaccaggag
     1921 gaggcaaaag tgaccgccaa taacgacaag aaccgcacct tctccgtctg gtacgtcccc
     1981 gaggtgacgg ggactcataa ggttactgtg ctctttgctg gccagcacat cgccaagagc
     2041 cccttcgagg tgtacgtgga taagtcacag ggtgacgcca gcaaagtgac agcccaaggt
     2101 cccggcctgg agcccagtgg caacatcgcc aacaagacca cctactttga gatctttacg
     2161 gcaggagctg gcacgggcga ggtcgaggtt gtgatccagg accccatggg acagaagggc
     2221 acggtagagc ctcagctgga ggcccggggc gacagcacat accgctgcag ctaccagccc
     2281 accatggagg gcgtccacac cgtgcacgtc acgtttgccg gcgtgcccat ccctcgcagc
     2341 ccctacactg tcactgttgg ccaagcctgt aacccgagtg cctgccgggc ggttggccgg
     2401 ggcctccagc ccaagggtgt gcgggtgaag gagacagctg acttcaaggt gtacacaaag
     2461 ggcgctggca gtggggagct gaaggtcacc gtgaagggcc ccaagggaga ggagcgcgtg
     2521 aagcagaagg acctggggga tggcgtgtat ggcttcgagt attaccccat ggtccctgga
     2581 acctatatcg tcaccatcac gtggggtggt cagaacatcg ggcgcagtcc cttcgaagtg
     2641 aaggtgggca ccgagtgtgg caatcagaag gtacgggcct ggggccctgg gctggagggc
     2701 ggcgtcgttg gcaagtcagc agactttgtg gtggaggcta tcggggacga cgtgggcacg
     2761 ctgggcttct cggtggaagg gccatcgcag gctaagatcg aatgtgacga caagggcgac
     2821 ggctcctgtg atgtgcgcta ctggccgcag gaggctggcg agtatgccgt tcacgtgctg
     2881 tgcaacagcg aagacatccg cctcagcccc ttcatggctg acatccgtga cgcgccccag
     2941 gacttccacc cagacagggt gaaggcacgt gggcctggat tggagaagac aggtgtggcc
     3001 gtcaacaagc cagcagagtt cacagtggat gccaagcacg gtggcaaggc cccacttcgg
     3061 gtccaagtcc aggacaatga aggctgccct gtggaggcgt tggtcaagga caacggcaat
     3121 ggcacttaca gctgctccta cgtgcccagg aagccggtga agcacacagc catggtgtcc
     3181 tggggaggcg tcagcatccc caacagcccc ttcagggtga atgtgggagc tggcagccac
     3241 cccaacaagg tcaaagtata cggccccgga gtagccaaga cagggctcaa ggcccacgag
     3301 cccacctact tcactgtgga ctgcgccgag gctggccagg gggacgtcag catcggcatc
     3361 aagtgtgccc ctggagtggt aggccccgcc gaagctgaca tcgacttcga catcatccgc
     3421 aatgacaatg acaccttcac ggtcaagtac acgccccggg gggctggcag ctacaccatt
     3481 atggtcctct ttgctgacca ggccacgccc accagcccca tccgagtcaa ggtggagccc
     3541 tctcatgacg ccagtaaggt gaaggccgag ggccctggcc tcagtcgcac tggtgtcgag
     3601 cttggcaagc ccacccactt cacagtaaat gccaaagctg ctggcaaagg caagctggac
     3661 gtccagttct caggactcac caagggggat gcagtgcgag atgtggacat catcgaccac
     3721 catgacaaca cctacacagt caagtacacg cctgtccagc agggtccagt aggcgtcaat
     3781 gtcacttatg gaggggatcc catccctaag agccctttct cagtggcagt atctccaagc
     3841 ctggacctca gcaagatcaa ggtgtctggc ctgggagaga aggtggacgt tggcaaagac
     3901 caggagttca cagtcaaatc aaagggtgct ggtggtcaag gcaaagtggc atccaagatt
     3961 gtgggcccct cgggtgcagc ggtgccctgc aaggtggagc caggcctggg ggctgacaac
     4021 agtgtggtgc gcttcctgcc ccgtgaggaa gggccctatg aggtggaggt gacctatgac
     4081 ggcgtgcccg tgcctggcag cccctttcct ctggaagctg tggcccccac caagcctagc
     4141 aaggtgaagg cgtttgggcc ggggctgcag ggaggcagtg cgggctcccc cgcccgcttc
     4201 accatcgaca ccaagggcgc cggcacaggt ggcctgggcc tgacggtgga gggcccctgt
     4261 gaggcgcagc tcgagtgctt ggacaatggg gatggcacat gttccgtgtc ctacgtgccc
     4321 accgagcccg gggactacaa catcaacatc ctcttcgctg acacccacat ccctggctcc
     4381 ccattcaagg cccacgtggt tccctgcttt gacgcatcca aagtcaagtg ctcaggcccc
     4441 gggctggagc gggccaccgc tggggaggtg ggccaattcc aagtggactg ctcgagcgcg
     4501 ggcagcgcgg agctgaccat tgagatctgc tcggaggcgg ggcttccggc cgaggtgtac
     4561 atccaggacc acggtgatgg cacgcacacc attacctaca ttcccctctg ccccggggcc
     4621 tacaccgtca ccatcaagta cggcggccag cccgtgccca acttccccag caagctgcag
     4681 gtggaacctg cggtggacac ttccggtgtc cagtgctatg ggcctggtat tgagggccag
     4741 ggtgtcttcc gtgaggccac cactgagttc agtgtggacg cccgggctct gacacagacc
     4801 ggagggccgc acgtcaaggc ccgtgtggcc aacccctcag gcaacctgac ggagacctac
     4861 gttcaggacc gtggcgatgg catgtacaaa gtggagtaca cgccttacga ggagggactg
     4921 cactccgtgg acgtgaccta tgacggcagt cccgtgccca gcagcccctt ccaggtgccc
     4981 gtgaccgagg gctgcgaccc ctcccgggtg cgtgtccacg ggccaggcat ccaaagtggc
     5041 accaccaaca agcccaacaa gttcactgtg gagaccaggg gagctggcac gggcggcctg
     5101 ggcctggctg tagagggccc ctccgaggcc aagatgtcct gcatggataa caaggacggc
     5161 agctgctcgg tcgagtacat cccttatgag gctggcacct acagcctcaa cgtcacctat
     5221 ggtggccatc aagtgccagg cagtcctttc aaggtccctg tgcatgatgt gacagatgcg
     5281 tccaaggtca agtgctctgg gcccggcctg agcccaggca tggttcgtgc caacctccct
     5341 cagtccttcc aggtggacac aagcaaggct ggtgtggccc cattgcaggt caaagtgcaa
     5401 gggcccaaag gcctggtgga gccagtggac gtggtagaca acgctgatgg cacccagacc
     5461 gtcaattatg tgcccagccg agaagggccc tacagcatct cagtactgta tggagatgaa
     5521 gaggtacccc ggagcccctt caaggtcaag gtgctgccta ctcatgatgc cagcaaggtg
     5581 aaggccagtg gccccgggct caacaccact ggcgtgcctg ccagcctgcc cgtggagttc
     5641 accatcgatg caaaggacgc cggggagggc ctgctggctg tccagatcac ggatcccgaa
     5701 ggcaagccga agaagacaca catccaagac aaccatgacg gcacgtatac agtggcctac
     5761 gtgccagacg tgacaggtcg ctacaccatc ctcatcaagt acggtggtga cgagatcccc
     5821 ttctccccgt accgcgtgcg tgccgtgccc accggggacg ccagcaagtg cactgtcaca
     5881 ggtgctggca tcggccccac cattcagatt ggggaggaga cggtgatcac tgtggacact
     5941 aaggcggcag gcaaaggcaa agtgacgtgc accgtgtgca cgcctgatgg ctcagaggtg
     6001 gatgtggacg tggtggagaa tgaggacggc actttcgaca tcttctacac ggccccccag
     6061 ccgggcaaat acgtcatctg tgtgcgcttt ggtggcgagc acgtgcccaa cagccccttc
     6121 caagtgacgg ctctggctgg ggaccagccc tcggtgcagc cccctctacg gtctcagcag
     6181 ctggccccac agtacaccta cgcccagggc ggccagcaga cttgggcccc ggagaggccc
     6241 ctggtgggtg tcaatgggct ggatgtgacc agcctgaggc cctttgacct tgtcatcccc
     6301 ttcaccatca agaagggcga gatcacaggg gaggttcgga tgccctcagg caaggtggcg
     6361 cagcccacca tcactgacaa caaagacggc accgtgaccg tgcggtatgc acccagcgag
     6421 gctggcctgc acgagatgga catccgctat gacaacatgc acatcccagg aagccccttg
     6481 cagttctatg tggattacgt caactgtggc catgtcactg cctatgggcc tggcctcacc
     6541 catggagtag tgaacaagcc tgccaccttc accgtcaaca ccaaggatgc aggagagggg
     6601 ggcctgtctc tggccattga gggcccgtcc aaagcagaaa tcagctgcac tgacaaccag
     6661 gatgggacat gcagcgtgtc ctacctgcct gtgctgccgg gggactacag cattctagtc
     6721 aagtacaatg aacagcacgt cccaggcagc cccttcactg cccgggtcac aggtgacgac
     6781 tccatgcgta tgtcccacct aaaggtcggc tctgctgccg acatccccat caacatctca
     6841 gagacggatc tcagcctgct gacggccact gtggtcccgc cctcgggccg ggaggagccc
     6901 tgtttgctga agcggctgcg taatggccac gtggggattt cattcgtgcc caaggagacg
     6961 ggggagcacc tggtgcatgt gaagaaaaat ggccagcacg tggccagcag ccccatcccg
     7021 gtggtgatca gccagtcgga aattggggat gccagtcgtg ttcgggtctc tggtcagggc
     7081 cttcacgaag gccacacctt tgagcctgca gagtttatca ttgatacccg cgatgcaggc
     7141 tatggtgggc tcagcctgtc cattgagggc cccagcaagg tggacatcaa cacagaggac
     7201 ctggaggacg ggacgtgcag ggtcacctac tgccccacag agccaggcaa ctacatcatc
     7261 aacatcaagt ttgccgacca gcacgtgcct ggcagcccct tctctgtgaa ggtgacaggc
     7321 gagggccggg tgaaagagag catcacccgc aggcgtcggg ctcctgcagt ggccaacgtt
     7381 ggtagtcatt gtgacctcag cctgaaaatc cctgaaatta gcatccagga tatgacagcc
     7441 caggtgacca gcccatcggg caagacccat gaggccgaga tcgtggaagg ggagaaccac
     7501 acctactgca tccgctttgt tcccgctgag atgggcacac acacagtcag cgtcaagtac
     7561 aagggccagc acgtgcctgg gagccccttc cagttcaccg tggggcccct aggggaaggg
     7621 ggagcccaca aggtccgagc tgggggccct ggcctggaga gagctgaagc tggagtgcca
     7681 gccgaattca gtatctggac ccgggaagct ggtgctggag gcctggccat tgctgtcgag
     7741 ggccccagca aggctgagat ctcttttgag gaccgcaagg acggctcctg tggtgtggct
     7801 tatgtggtcc aggagccagg tgactacgaa gtctcagtca agttcaacga ggaacacatt
     7861 cccgacagcc ccttcgtggt gcctgtggct tctccgtctg gcgacgcccg ccgcctcact
     7921 gtttctagcc ttcaggagtc agggctaaag gtcaaccagc cagcctcttt tgcagtcagc
     7981 ctgaacgggg ccaagggggc gatcgatgcc aaggtgcaca gcccctcagg agccctggag
     8041 gagtgctatg tcacagaaat tgaccaagat aagtatgctg tgcgcttcat ccctcgggag
     8101 aatggcgttt acctgattga cgtcaagttc aacggtaccc acatccctgg aagccccttc
     8161 aagatccgag ttggggagcc tgggcatgga ggggacccag gcttggtgtc tgcttacgga
     8221 gcaggtctgg aaggcggtgt cacagggaac ccagctgagt tcgtcgtgaa cacgagcaat
     8281 gcgggagctg gtgccctgtc ggtgaccatt gacggcccct ccaaggtgaa gatggattgc
     8341 caggagtgcc ctgagggcta ccgcgtcacc tataccccca tggcacctgg cagctacctc
     8401 atctccatca agtacggcgg cccctaccac attgggggca gccccttcaa ggccaaagtc
     8461 acaggccccc gtctcgtcag caaccacagc ctccacgaga catcatcagt gtttgtagac
     8521 tctctgacca aggccacctg tgccccccag catggggccc cgggtcctgg gcctgctgac
     8581 gccagcaagg tggtggccaa gggcctgggg ctgagcaagg cctacgtagg ccagaagagc
     8641 agcttcacag tagactgcag caaagcaggc aacaacatgc tgctggtggg ggttcatggc
     8701 ccaaggaccc cctgcgagga gatcctggtg aagcacgtgg gcagccggct ctacagcgtg
     8761 tcctacctgc tcaaggacaa gggggagtac acactggtgg tcaaatgggg gcacgagcac
     8821 atcccaggca gcccctaccg cgttgtggtg ccctgagtct ggggcccgtg ccagccggca
     8881 gcccccaagc ctgccccgct acccaagcag ccccgccctc ttcccctcaa ccccggccca
     8941 ggccgccctg gccgcccgcc tgtcactgca gctgcccctg ccctgtgccg tgctgcgctc
     9001 acctgcctcc ccagccagcc gctgacctct cggctttcac ttgggcagag ggtagctgac
     9061 tagcacaatg gatctcgagg gatcttccat acctaccagt tctgcgcctg caggtcgcgg
     9121 ccgctcgagg ccggcaaggc cggatccaga catgataaga tacattgatg agtttggaca
     9181 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     9241 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     9301 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     9361 atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     9421 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     9481 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgagg
     9541 tcgactctag agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc
     9601 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     9661 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     9721 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     9781 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     9841 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     9901 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     9961 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
    10021 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
    10081 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    10141 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    10201 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
    10261 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
    10321 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    10381 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
    10441 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
    10501 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
    10561 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
    10621 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
    10681 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
    10741 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
    10801 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
    10861 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
    10921 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
    10981 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
    11041 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
    11101 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
    11161 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
    11221 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
    11281 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
    11341 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
    11401 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
    11461 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
    11521 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
    11581 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
    11641 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
    11701 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    11761 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
    11821 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    11881 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    11941 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    12001 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    12061 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    12121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    12181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    12241 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    12301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    12361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    12421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    12481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    12541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    12601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    12661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    12721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    12781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    12841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    12901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    12961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    13021 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    13081 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    13141 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    13201 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    13261 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    13321 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    13381 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    13441 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    13501 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    13561 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    13621 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    13681 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    13741 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    13801 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    13861 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    13921 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    13981 atttccccga aaagtgccac ctgacgtc
//
