LOCUS       sequence-332906-        5669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express ExbB delta(195-204),ExbD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5669)
  TITLE     Postle Lab Plasmids
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKP1963
FEATURES             Location/Qualifiers
     source          1..5669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(1527..1544)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1697..1783
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1875..1902
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1921..2012
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2013..2081,2082..2873)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2231..2250)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2915..3370
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3002..3021)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3212..3233
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      3481..4069
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3970..3989
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4223..4240
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4406..4428
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4566..4585
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(4765..5643)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
      301 ctagcaggag gaattcacca tgggtaataa tttaatgcag acggaccttt ccgtctgggg
      361 tatgtatcag cacgccgata ttgtcgttaa gtgcgtgatg attgggctta ttttggcctc
      421 cgtagtcacc tgggcaatct tcttcagtaa gagcgtagag ttcttcaatc agaagcgtcg
      481 ccttaagcgc gagcagcaac tgctggctga agcgcgttcc ttaaaccagg ccaacgatat
      541 cgccgctgat tttggtagca aaagcttaag cctgcatttg ctcaatgaag cgcagaatga
      601 gctggaactg tcagaaggca gcgacgataa cgaaggtatt aaagaacgta ccagcttccg
      661 tctggagcgt cgggtcgccg cagtgggtcg tcaaatgggc cgcggtaacg gctatctggc
      721 aaccatcggc gcgatttctc cgttcgttgg tctgtttggt acggtctggg gcatcatgaa
      781 cagctttatt ggtatcgcgc aaacgcagac cactaacctg gcagtcgttg cgccgggtat
      841 cgcagaagct ctgttagcaa cggcaatcgg cctcgtggca gcgattcctg cggtcgttat
      901 ctttaaagcg atgctgggtg atgttgcagc gcaggtattg ttgctgcaaa gccgtgacct
      961 ggatctggaa gccagcgccg ctgcgcatcc ggttcgtgtc gcacaaaaat tacgcgcagg
     1021 ataatatccg atggcaatgc atcttaacga aaacctcgac gataacggcg aaatgcatga
     1081 tatcaacgtg acgccgttta tcgacgtgat gttggttctg ctgattatct ttatggtggc
     1141 ggcaccgtta gcgacggtag atgtgaaggt gaacttgcct gcttctacca gcacgccgca
     1201 gccgcgcccg gaaaaaccgg tttatctgtc ggtgaaggca gacaactcga tgtttatcgg
     1261 taacgatccg gtcaccgatg aaacaatgat tacggcgttg aatgcgttaa ccgaaggcaa
     1321 gaaagacacc accatcttct tccgagcgga caaaaccgtc gattacgaga cgttgatgaa
     1381 ggtaatggat acgctgcatc aggcgggtta cctgaagata ggtctggtcg gcgaagaaac
     1441 cgccaaagcg aagtaagggg atcctctaga gtcgacctgc aggcatgcaa gcttggctgt
     1501 tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     1561 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
     1621 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
     1681 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1741 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     1801 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     1861 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct
     1921 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1981 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2041 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2101 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2161 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2221 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     2281 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2341 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2401 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2461 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     2521 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     2581 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     2641 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2701 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2761 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2821 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2881 accaagttta ctcatatata ctttagattg atttacgcgc cctgtagcgg cgcattaagc
     2941 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     3001 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     3061 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     3121 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     3181 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     3241 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     3301 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     3361 tttacaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3421 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3481 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3541 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3601 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     3661 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3721 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3781 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3841 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3901 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3961 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4021 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4081 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4141 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4201 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     4261 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatagggt catggctgcg
     4321 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4381 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4441 tcaccgaaac gcgcgaggca gaaggagatg gcgcccaaca gtcccccggc cacggggcct
     4501 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
     4561 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
     4621 gccacgatgc gtccggcgta gaggatctgc tcatgtttga cagcttatca tcgatgcata
     4681 atgtgcctgt caaatggacg aagcagggat tctgcaaacc ctatgctact ccgtcaagcc
     4741 gtcaattgtc tgattcgtta ccaattatga caacttgacg gctacatcat tcactttttc
     4801 ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga
     4861 gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt
     4921 ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct
     4981 aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg
     5041 tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc
     5101 ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg
     5161 cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg
     5221 cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg
     5281 gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc
     5341 gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta
     5401 gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg
     5461 tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat
     5521 tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc
     5581 cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc
     5641 catacttttc atactcccgc cattcagag
//
