LOCUS       sequence-333039-        8247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPenguin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8247)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmPenguin_B
FEATURES             Location/Qualifiers
     source          1..8247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..31
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        95..2604
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2624..2626,2627..2629,2630..3340)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2624..2626,2627..2629,2630..3340)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2669..2690)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2930..2949)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3277..3298
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5592..5633
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5643..5660
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5683..5700)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5733..5867
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5757..5776)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5811..5830
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6468..7056)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6548..6567)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7227..8018,8019..8087))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7850..7869
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8088..8192)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt aattctatat
       61 tctaaaaaca caaatgatac ttctaaaaaa aaatcatgaa tggcatcaac tctgaatcaa
      121 atctttgcag atgcacctac ttctcatttc cactgtcaca tcatttttcc agatctcgct
      181 gcctgttatg tggcccacaa accaagacac gttttatggc cattaaagct ggctgatcgt
      241 cgccaaacac caaatacata atgaatatgt acacattcga gaaagaagcg atcaaagaag
      301 cgtcttcggg cggagtagga gaatgcggag gagaaggaga acgagctgat ctagtatctc
      361 tccacaatcc aatgccaact gaccaactgg ccatattcgg agcaatttga agccaatttc
      421 catcgcctgg cgatcgctcc attcttggct atatgttttt caccgttacc cggggccatt
      481 ttcaaagact cgtcggcaag ataagattgt gtcactcgct gtctctcttc atttgtcgaa
      541 gaatgctgag gaatttcgcg atgacgtcgg cgagtatttt gaagaatgag aataatttgt
      601 atttatacga aaatcagtta gtggaatttt ctacaaaaac atgttatcta tagataattt
      661 tgttgcaaaa tatgttgact atgacaaaga ttgtatgtat atacctttaa tgtattctca
      721 ttttcttatg tatttataat ggcaatgatg atactgatga tattttaaga tgatgccaga
      781 ccaaaaggct tgaatttctg cgtcttttgc cgaacgcagt gcatgtgcaa ttgttgtttt
      841 ttggaatatt caattttcgg actgtccgct ttgatttcag tttcttggct tattcaaaaa
      901 gcaaagtaaa gccaaaaaag cgagatggca ataccaaatg cggcaaaacg gtagtggaag
      961 gaaaggggtg cggggcagcg gaaggaaggg tggggcgggg cgtggcgggg tctgtggctg
     1021 ggcgcgacgt caccgacgtt ggagccactc ctttgaccat gtgtgcgtgt gtgtattatt
     1081 cgtgtctcgc cactcgccgg ttgttttttt ctttttatgc tgcgctctct ctagcgccat
     1141 ctcgcttacg catgctcaac gcaccgcatg ttgccgtttc cttttatgcg tcattttggc
     1201 tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa taaataagtc
     1261 tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa aagcaacaac
     1321 acaggtggct ttccctcttt cactttttgt ttgcaagccg cgtgcgagca agacggcacg
     1381 accggcaaac gcaattacgc tgacaaagag cagacgaagt tttggcgaaa aacatcaagg
     1441 cgcctgatac gaatgcattt gcaataacaa ttgcgatatt taatattgtt tatgaagctg
     1501 tttgacttca aaacacacaa aaaaaaaaat aaaacaaatt atttgaaaga gaattaggaa
     1561 tcggacgctt atcgttaggg taacaacaag aaatgcttac tgagtcacag cctctggaaa
     1621 actgccgcaa gccagagaga gagagaaaaa gagggagagc agcttagacc gcatgtgctt
     1681 gtgtgtgagg cgtctctctc ttcgtctctg ttgcgcaaac gcatagactg cactgaaaaa
     1741 atcgattacc tattttttat gaatgaatat ttgcactatt actattcaaa actattaaga
     1801 tagcaatcac attcaatagc caaatactat accacctgag cgatgcaacg aaatgatcaa
     1861 tttgagcaaa aatgctgcat atttaggacg gcatcattat agaaatgctt cttgctgtgt
     1921 acttttctct cgtctggcag ctgtttcgcc gttattgtta aaaccggctt aagttaggtg
     1981 tgttttctac gactagtgaa tgccctacta gaagatgtgt gttgcacaaa atgtccctgg
     2041 aataaccaat ttgaagtgca gatagcagta aacgtaagct aatatgaata ttatttaact
     2101 gtaatgtttt aatatcgctg gacattacta ataaacccac tataaacaca tgtacatatg
     2161 tatgttttgg catacaatga gtagttgggg aaaaaatgtg taaaagcacc gtgaccatca
     2221 cagcataaag ataaccagct gaagtatcga atatgagtaa cccccaaatt gaatcacatg
     2281 ccgcaactga taggacccat ggaagtacac tcttcatggc gatatacaag acacacacaa
     2341 gcacgaacac ccagttgcgg aggaaattct ccgtaaatga aaacccaatc ggcgaacaat
     2401 tcatacccat atatggtaaa agttttgaac gcgacttgag agcggagagc attgcggctg
     2461 ataaggtttt agcgctaagc gggctttata aaacgggctg cgggaccagt tttcatatca
     2521 ctaccgtttg agttcttgtg ctgtgtggat actcctcccg acacaaagcc gctccatcag
     2581 ccagcagtcg tctaatccag agaccccgga tcggggtacc atcatggtga gcaagggcga
     2641 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2701 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2761 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     2821 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2881 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2941 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3001 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3061 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     3121 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3181 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3241 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3301 cgccgccggg atcactctcg gcatggacga gctgtacaag ccaagcttga tggttagctc
     3361 ggagccgaaa ggacctgcta acgtggccaa cacggcctcg cccctcaagg cccatgaatc
     3421 gtccggcaag cgcgccaacg gacagaagtt taagcccggt ggagcaggag gcgccaggaa
     3481 gttcgacaaa ttcggcggag gagcaggcaa gtcgtttgta aagccaggcg ctggtggtgc
     3541 caagaagttc gacaagtctg ggcccggtgg cttcaagaag tttgacaaat ccggagccac
     3601 cggcgacaag aggcttggtg gcaacaagtt ccgcggaaaa ccacaaacac agccacaggc
     3661 gccggcggag ggcgagaaac aggactggaa caagttcaag aaggagaaga aggacctcaa
     3721 gctgaagcgc aagagtgcca aggacaccta cgagatcagc aaggaggcca accagatcca
     3781 cgagaagttg cgatgccgcc gaacggagaa caaggacaag ctggtggagc agatatacaa
     3841 ggtgctcaac gtgggcgaca ctatttcaaa ggtggtgaag gcccacgaca ccgcccgcgt
     3901 gatccagagc atgctaaagt acgcgtcgcc agctctgcgg gccgagatct ctgagaagct
     3961 gcttcccttc accgtggaga tgtgccagtc gaaatacgcc cagttctgcg tgcaacggat
     4021 gctcaagtac ggtgcgccag ccaccaaagc caagctggtg gacagcctgt acggacatat
     4081 agtgcgtttg gccggacaca gcatcggcag tgggctgctg gattcgatgt atcagagcgc
     4141 cacacccaat cagcgcatct atatgcgcca ggagttctac ggcgatctgt accggaaggc
     4201 caaggattcg aatgtgaaga ctctgagcga cacctataag gaggccacga acatgaaggc
     4261 ctccattttg gggtcggtta aagcaaacct ggatcatgtg gccaacaagc agctggtgga
     4321 cagtgcgctg gtgcacgccg tgatgctgga gtacctgcgc gcctgcgacg aagatgagga
     4381 gaagctggag gagacggtca ctgcgtttgc ggctctagtg cctcacatgc tgtccaccaa
     4441 ggagggcagc gaggcggctg ttatatgctt ctacaagtcg acgcccaaga atcgcagagc
     4501 cattatcaag aatatcaagg agcacctgct aaagatcgcc aaccatgagc acggccacgt
     4561 gttcctcatc tcgctgctaa acgcactgga cgacactaag gcgaccaaga aggcaattta
     4621 cgatcaccta cacggagatc taaaggcgct aatgagcagc ccgtacggcc gacgagtgat
     4681 tcaatggctg gtggctccgg gtgatacgac atgcttccat ccagagttca ttcgcacggt
     4741 ggaggagggt ctagcctttg gaaaaaagga gaaggagctg cgtcgcaagg agatccttga
     4801 gcagatagag gcgccgattg cacagtccat cgccgaggat gccgcctttt ggctgtccaa
     4861 cagccacatc ggcctggtca ccggcgatat tcttaatcac attcaaggcg agtcctatga
     4921 gaaggctgct tcggcccttg cccaagtcgt cgtccaaccg gagtggcgca tttcagcaga
     4981 cgccgccggt ccgcagccgc aggataagaa gaagcctcac aatgacgtgg aggccattat
     5041 agcccaggcc accaaacagc gcagaaaact gctttatgtc gagagcagca gcgacgacga
     5101 agatgaagat gaagatgaag atgaggagag cgacgatgaa ggagacgaga aggagcagaa
     5161 ggaggcggcc gcagatgatg ccgaacccaa ggtgaagaaa gccaaaaagg agcccaaaaa
     5221 gccaaaggca aaggaggagg agccggcggc tccgttggtc tccggaatcg aggaggccgg
     5281 catgcacatt gtattgaaga agatactcaa gaacgatggc aagcgagagg gcaccccgtt
     5341 cagccaacag ctactccaaa acttgtcctc cgatgtgctg aaagcctggc tgggcgtcaa
     5401 ccgggcctgt tttgtcctgc tcaagctggt ggaggagtgt cccgcactgc tggacgactg
     5461 caagaaagcc atcgcggcgg agaggagcct tagccagata ctcgcggacc gtaagacgcc
     5521 cggagccaag ctactggccg ccaaactgga cattggcaaa tgactcgagt ctagagggcc
     5581 cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     5641 gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta
     5701 aggcctgagc tcgctgatca gcctcgatcg aggatccaga catgataaga tacattgatg
     5761 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     5821 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     5881 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     5941 acctctacaa atgtggtatg gctgattatg atcagtcgac cgatgccctt gagagccttc
     6001 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     6061 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc
     6121 gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc
     6181 ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag
     6241 caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg
     6301 acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg
     6361 cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa
     6421 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6481 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6541 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6601 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6661 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6721 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6781 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6841 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6901 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6961 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7021 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7081 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7141 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7201 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7261 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7321 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7381 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7441 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7501 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     7561 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7621 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7681 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7741 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7801 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     7861 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7921 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7981 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8041 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8101 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8161 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8221 gtctaagaaa ccattattat catgaca
//
