LOCUS       sequence-333048-       13146 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for S of SARS-CoV-2 (B.1.351 lineage / 
            South African variant) with N-terminal c-myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Zhang L, Dutta S, Xiong S, Chan M, Chan KK, Fan TM, Bailey KL, 
            Lindeblad M, Cooper LM, Rong L, Gugliuzza AF, Shukla D, Procko E, 
            Rehman J, Malik AB
  TITLE     Engineered ACE2 decoy mitigates lung injury and death induced by 
            SARS-CoV-2 variants.
  JOURNAL   Nat Chem Biol. 2022 Mar;18(3):342-351. doi: 
            10.1038/s41589-021-00965-6. Epub 2022 Jan 19.
  PUBMED    35046611
REFERENCE   2  (bases 1 to 13146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00965-6"; journalName: "Nat Chem Biol"; date: 
            "2022-03"; volume: "18"; issue: "3"; pages: "342-351"
COMMENT     Sequence Label: pCEP4-myc-S (B.1.351 lineage / South African
            v...
FEATURES             Location/Qualifiers
     source          1..13146
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        383..762
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        763..966
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     917..937
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     963..987
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1069..1098
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    4904..5038
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4928..4947)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4982..5001
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5181..5203)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5793..7582
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(9725..9743)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9811..9915
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9916..9984,9985..10776)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10134..10153)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10947..11535
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11436..11455
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        11560..11705
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             11748..12785
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(12783..12802)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    12827..12875
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gagcagacag acccatggtt tttggatggc ctgggcatgg accgcatgta ctggcgcgac
       61 acgaacaccg ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc accgcgcgga
      121 tttctggcgt gccaagctag tcgaccaatt ctcatgtttg acagcttatc atcgcagatc
      181 cgggcaacgt tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca
      241 ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt
      301 ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta tattggctca
      361 tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
      421 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      481 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      541 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      601 actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc
      661 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct
      721 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      781 tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      841 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat
      901 aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      961 agagctcgtt tagtgaaccg tcagatctct agaagctggg taccagctgc tagcgccacc
     1021 atgaagacca tcatcgccct gagctacatc ttctgcctgg tgttcgccga gcaaaagctg
     1081 atctcagagg aggacctggg cagccctggc ggtgccgtca atttcacaac tcggactcag
     1141 ctgccacctg cttatactaa tagcttcacc agaggcgtgt actatcctga caaggtgttt
     1201 agaagctccg tgctgcactc tacacaggat ctgtttctgc cattctttag caacgtgacc
     1261 tggttccacg ccatccacgt gagcggcacc aatggcacaa agcggttcgc caatcccgtg
     1321 ctgcctttta acgatggcgt gtacttcgcc tctaccgaga agagcaacat catcagaggc
     1381 tggatctttg gcaccacact ggactccaag acacagtctc tgctgatcgt gaacaatgcc
     1441 accaacgtgg tcatcaaggt gtgcgagttc cagttttgta atgatccctt cctgggcgtg
     1501 tactatcaca agaacaataa gagctggatg gagtccgagt ttagagtgta ttctagcgcc
     1561 aacaactgca catttgagta cgtgagccag cctttcctga tggacctgga gggcaagcag
     1621 ggcaatttca agaacctgag ggagttcgtg tttaagaata tcgacggcta cttcaaaatc
     1681 tactctaagc acacccccat caacctggtg cgcggcctgc ctcagggctt cagcgccctg
     1741 gagcccctgg tggatctgcc tatcggcatc aacatcaccc ggtttcagac actgcacaga
     1801 agctacctga cacccggcga ctcctctagc ggatggaccg ccggcgctgc cgcctactat
     1861 gtgggctacc tccagccccg gaccttcctg ctgaagtaca acgagaatgg caccatcaca
     1921 gacgcagtgg attgcgccct ggaccccctg agcgagacaa agtgtacact gaagtccttt
     1981 accgtggaga agggcatcta tcagacatcc aatttcaggg tgcagccaac cgagtctatc
     2041 gtgcgctttc ctaatatcac aaacctgtgc ccatttggcg aggtgttcaa cgcaacccgc
     2101 ttcgccagcg tgtacgcctg gaataggaag cggatcagca actgcgtggc cgactatagc
     2161 gtgctgtaca actccgcctc tttcagcacc tttaagtgct atggcgtgtc ccccacaaag
     2221 ctgaatgacc tgtgctttac caacgtctac gccgattctt tcgtgatcag gggcgacgag
     2281 gtgcgccaga tcgcccccgg ccagacaggc aacatcgcag actacaatta taagctgcca
     2341 gacgatttca ccggctgcgt gatcgcctgg aacagcaaca atctggattc caaagtgggc
     2401 ggcaactaca attatctgta ccggctgttt agaaagagca atctgaagcc cttcgagagg
     2461 gacatctcta cagaaatcta ccaggccggc agcacccctt gcaatggcgt gaagggcttt
     2521 aactgttatt tcccactcca gtcctacggc ttccagccca catacggcgt gggctatcag
     2581 ccttaccgcg tggtggtgct gagctttgag ctgctgcacg ccccagcaac agtgtgcggc
     2641 cccaagaagt ccaccaatct ggtgaagaac aagtgcgtga acttcaactt caacggcctg
     2701 accggcacag gcgtgctgac cgagtccaac aagaagttcc tgccatttca gcagttcggc
     2761 agggacatcg cagataccac agacgccgtg cgcgacccac agaccctgga gatcctggac
     2821 atcacaccct gctctttcgg cggcgtgagc gtgatcacac ccggcaccaa tacaagcaac
     2881 caggtggccg tgctgtatca gggcgtgaat tgtaccgagg tgcccgtggc tatccacgcc
     2941 gatcagctga ccccaacatg gcgggtgtac agcaccggct ccaacgtctt ccagacaaga
     3001 gccggatgcc tgatcggagc agagcacgtg aacaattcct atgagtgcga catcccaatc
     3061 ggcgccggca tctgtgcctc ttaccagacc cagacaaact ctcccagaag agcccggagc
     3121 gtggcctccc agtctatcat cgcctatacc atgtccctgg gcgtcgagaa cagcgtggcc
     3181 tactctaaca atagcatcgc catcccaacc aacttcacaa tctctgtgac cacagagatc
     3241 ctgcccgtgt ccatgaccaa gacatctgtg gactgcacaa tgtatatctg tggcgattct
     3301 accgagtgca gcaacctgct gctccagtac ggcagctttt gtacccagct gaatagagcc
     3361 ctgacaggca tcgccgtgga gcaggataag aacacacagg aggtgttcgc ccaggtgaag
     3421 caaatctaca agaccccccc tatcaaggac tttggcggct tcaatttttc ccagatcctg
     3481 cctgatccat ccaagccttc taagcggagc tttatcgagg acctgctgtt caacaaggtg
     3541 accctggccg atgccggctt catcaagcag tatggcgatt gcctgggcga catcgcagcc
     3601 agggacctga tctgcgccca gaagtttaat ggcctgaccg tgctgccacc cctgctgaca
     3661 gatgagatga tcgcacagta cacaagcgcc ctgctggccg gcaccatcac atccggatgg
     3721 accttcggcg caggagccgc cctccagatc ccctttgcca tgcagatggc ctataggttc
     3781 aacggcatcg gcgtgaccca gaatgtgctg tacgagaacc agaagctgat cgccaatcag
     3841 tttaactccg ccatcggcaa gatccaggac agcctgtcct ctacagccag cgccctgggc
     3901 aagctccagg atgtggtgaa tcagaacgcc caggccctga ataccctggt gaagcagctg
     3961 agcagcaact tcggcgccat ctctagcgtg ctgaatgaca tcctgagccg gctggacaag
     4021 gtggaggcag aggtgcagat cgaccggctg atcaccggcc ggctccagag cctccagacc
     4081 tatgtgacac agcagctgat cagggccgcc gagatcaggg ccagcgccaa tctggcagca
     4141 atcaagatgt ccgagtgcgt gctgggccag tctaagagag tggacttttg tggcaagggc
     4201 tatcacctga tgtccttccc tcagtctgcc ccacacggcg tggtgtttct gcacgtgacc
     4261 tacgtgcccg cccaggagaa gaacttcacc acagcccctg ccatctgcca cgatggcaag
     4321 gcccactttc caagggaggg cgtgttcgtg tccaacggca cccactggtt tgtgacacag
     4381 cgcaatttct acgagcccca gatcatcacc acagacaaca ccttcgtgag cggcaactgt
     4441 gacgtggtca tcggcatcgt gaacaatacc gtgtatgatc cactccagcc cgagctggac
     4501 agctttaagg aggagctgga taagtatttc aagaatcaca cctcccctga cgtggatctg
     4561 ggcgacatca gcggcatcaa tgcctccgtg gtgaacatcc agaaggagat cgaccgcctg
     4621 aacgaggtgg ctaagaatct gaacgagagc ctgatcgacc tccaggagct gggcaagtat
     4681 gagcagtaca tcaagtggcc ctggtacatc tggctgggct tcatcgccgg cctgatcgcc
     4741 atcgtgatgg tgaccatcat gctgtgctgt atgacatcct gctgttcttg cctgaagggc
     4801 tgctgtagct gtggctcctg ctgtaagttt gacgaggatg actctgaacc tgtgctgaag
     4861 ggcgtgaagc tgcattacac ctaactcgag gccggcaagg ccggatccag acatgataag
     4921 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     4981 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     5041 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     5101 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatccggctg cctcgcgcgt
     5161 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     5221 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     5281 tgtcggggcg cagccatgag gtcgactcta gaggatcgat ccccgccccg gacgaactaa
     5341 acctgactac smcmkcycyg smcswwctwm rcskgactac gacatctctg ccccttcttc
     5401 gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt
     5461 ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc
     5521 ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg
     5581 cagtctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac
     5641 accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca
     5701 acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca
     5761 atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt
     5821 agtatatact atccagacta accctaattc aatagcatat gttacccaac gggaagcata
     5881 tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg
     5941 agctgtcacg gttttattta catggggtca ggattccacg agggtagtga accattttag
     6001 tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct
     6061 gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt
     6121 gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg
     6181 ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca
     6241 ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg
     6301 tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca
     6361 cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag
     6421 acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac
     6481 agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca
     6541 atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc
     6601 acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac
     6661 ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa
     6721 tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc
     6781 tgcgatctgg aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg
     6841 tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat
     6901 actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta
     6961 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     7021 tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     7081 tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta
     7141 tcctaataga gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta
     7201 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct
     7261 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     7321 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     7381 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     7441 aatctgtatc cgggtagcat atgctatcct catgcatata cagtcagcat atgataccca
     7501 gtagtagagt gggagtgcta tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt
     7561 ttcgctgctt gtccttttcc tgctggttgc tcccattctt aggtgaattt aaggaggcca
     7621 ggctaaagcc gtcgcatgtc tgattgctca ccaggtaaat gtcgctaatg ttttccaacg
     7681 cgagaaggtg ttgagcgcgg agctgagtga cgtgacaaca tgggtatgcc caattgcccc
     7741 atgttgggag gacgaaaatg gtgacaagac agatggccag aaatacacca acagcacgca
     7801 tgatgtctac tggggattta ttctttagtg cgggggaata cacggctttt aatacgattg
     7861 agggcgtctc ctaacaagtt acatcactcc tgcccttcct caccctcatc tccatcacct
     7921 ccttcatctc cgtcatctcc gtcatcaccc tccgcggcag ccccttccac cataggtgga
     7981 aaccagggag gcaaatctac tccatcgtca aagctgcaca cagtcaccct gatattgcag
     8041 gtaggagcgg gctttgtcat aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat
     8101 atatgagttt gtaaaaagac catgaaataa cagacaatgg actcccttag cgggccaggt
     8161 tgtgggccgg gtccaggggc cattccaaag gggagacgac tcaatggtgt aagacgacat
     8221 tgtggaatag caagggcagt tcctcgcctt aggttgtaaa gggaggtctt actacctcca
     8281 tatacgaaca caccggcgac ccaagttcct tcgtcggtag tcctttctac gtgactccta
     8341 gccaggagag ctcttaaacc ttctgcaatg ttctcaaatt tcgggttgga acctccttga
     8401 ccacgatgct ttccaaacca ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg
     8461 ggtccagtgc ttgggccttc tcctgggtca tctgcggggc cctgctctat cgctcccggg
     8521 ggcacgtcag gctcaccatc tgggccacct tcttggtggt attcaaaata atcggcttcc
     8581 cctacagggt ggaaaaatgg ccttctacct ggagggggcc tgcgcggtgg agacccggat
     8641 gatgatgact gactactggg actcctgggc ctcttttctc cacgtccacg acctctcccc
     8701 ctggctcttt cacgacttcc ccccctggct ctttcacgtc ctctaccccg gcggcctcca
     8761 ctacctcctc gaccccggcc tccactacct cctcgacccc ggcctccact gcctcctcga
     8821 ccccggcctc cacctcctgc tcctgcccct cctgctcctg cccctcctcc tgctcctgcc
     8881 cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctcctgc ccctcctgcc
     8941 cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc
     9001 cctcccgctc ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt
     9061 ggacgttttt ggggtctccg gacaccatct ctatgtcttg gccctgatcc tgagccgccc
     9121 ggggctcctg gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta
     9181 tcaccccctc ttctttgagg tccactgccg ccggagcctt ctggtccaga tgtgtctccc
     9241 ttctctccta ggccatttcc aggtcctgta cctggcccct cgtcagacat gattcacact
     9301 aaaagagatc aatagacatc tttattagac gacgctcagt gaatacaggg agtgcagact
     9361 cctgccccct ccaacagccc ccccaccctc atccccttca tggtcgctgt cagacagatc
     9421 caggtctgaa aattccccat cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag
     9481 cccggaaaac tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa gccaggcctc
     9541 aaattcctcg tccccctttt tgctggacgg tagggatggg gattctcggg acccctcctc
     9601 ttcctcttca aggtcaccag acagagatgc tactggggca acggaagaaa agctgggtgc
     9661 ggcctgtgag gatcagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga
     9721 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     9781 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     9841 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     9901 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     9961 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
    10021 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
    10081 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
    10141 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
    10201 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
    10261 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
    10321 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
    10381 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
    10441 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
    10501 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
    10561 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
    10621 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
    10681 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
    10741 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
    10801 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    10861 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    10921 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    10981 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    11041 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
    11101 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    11161 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    11221 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    11281 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    11341 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    11401 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    11461 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    11521 cggagcctat ggaaaaacgc cagcaacgcg gcctttttga tgacacaaac cccgcccagc
    11581 gtcttgtcat tggcgaattc gaacacgcag atgcagtcgg ggcggcgcgg tccgaggtcc
    11641 acttcgcata ttaaggtgac gcgtgtggcc tcgaacaccg agcgaccctg cagcgacccg
    11701 cttaacagcg tcaacagcgt gccgcagatc ccggggggca atgagatatg aaaaagcctg
    11761 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
    11821 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
    11881 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
    11941 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
    12001 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
    12061 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
    12121 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
    12181 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
    12241 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
    12301 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
    12361 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
    12421 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
    12481 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
    12541 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
    12601 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
    12661 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
    12721 tcgccgatag tggaaaccga cgccccagca ctcgtccgga tcgggagatg ggggaggcta
    12781 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
    12841 cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
    12901 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
    12961 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
    13021 ggcggcaggc cctgccatag ccactggccc cgtgggttag ggacggggtc ccccatgggg
    13081 aatggtttat ggttcgtggg ggttattatt ttgggcgttg cgtggggtca ggtccacgac
    13141 tggact
//
