LOCUS       sequence-33307-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  TITLE     Double Floxed Inverted ORF Control
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-Ef1a-DIO EYFP
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..59
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    110..143
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     intron          183..386
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(206..226)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 acgagctgta caagtaaggc gcgccataac ttcgtataat gtatgctata cgaagttatt
       61 aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgca taacttcgta
      121 taaagtatcc tatacgaagt tattccggag tcgactctag aggatccggt acctcacgac
      181 acctgaaatg gaagaaaaaa actttgaacc actgtctgag gcttgagaat gaaccaagat
      241 ccaaactcaa aaagggcaaa ttccaaggag aattacatca agtgccaagc tggcctaact
      301 tcagtctcca cccactcagt gtggggaaac tccatcgcat aaaacccctc cccccaacct
      361 aaagacgacg tactccaaaa gctcgagaac taatcgaggt gcctggacgg cgcccggtac
      421 tccgtggagt cacatgaagc gacggctgag gacggaaagg cccttttcct ttgtgtgggt
      481 gactcacccg cccgctctcc cgagcgccgc gtcctccatt ttgagctccc tgcagcaggg
      541 ccgggaagcg gccatctttc cgctcacgca actggtgccg accgggccag ccttgccgcc
      601 cagggcgggg cgatacacgg cggcgcgagg ccaggcacca gagcaggccg gccagcttga
      661 gactaccccc gtccgattct cggtggccgc gctcgcaggc cccgcctcgc cgaacatgtg
      721 cgctgggacg cacgggcccc gtcgccgccc gcggccccaa aaaccgaaat accagtgtgc
      781 agatcttggc ccgcatttac aagactatct tgccagaaaa aaagcgtcgc agcaggtcat
      841 caaaaatttt aaatggctag agacttatcg aaagcagcga gacaggcgcg aaggtgccac
      901 cagattcgca cgcggcggcc ccagcgccca ggccaggcct caactcaa
//
