LOCUS       sequence-333098-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted 
            between D139 and E140, as well as C61S and C261S in HaloTag7 to 
            block disulfide bond formation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Keeble AH, Yadav VK, Ferla MP, Bauer CC, Chuntharpursat-Bon E, Huang
            J, Bon RS, Howarth M
  TITLE     DogCatcher allows loop-friendly protein-protein ligation.
  JOURNAL   Cell Chem Biol. 2021 Jul 28. pii: S2451-9456(21)00315-9. doi: 
            10.1016/j.chembiol.2021.07.005.
  PUBMED    34324879
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2021 Jul 28. pii: S2451-9456(21)00315-9. doi: 
            10.1016/j.chembiol.2021.07.005."
COMMENT     Sequence Label: pET28a-HaloTag7SS-DogTag
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             469..537
                     /codon_start=1
                     /product="peptide derived from the RrgA adhesin of 
                     Streptococcus pneumoniae"
                     /label=DogTag
                     /note="forms a covalent bond with the DogCatcher protein 
                     when inserted into an intra-domain protein loop (Keeble et 
                     al., 2022)"
                     /translation="DIPATYEFTDGKHYITNEPIPPK"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcg aaatcggtac tggctttcca
       61 ttcgaccccc attatgtgga agtcctgggc gagcgcatgc actacgtcga tgttggtccg
      121 cgcgatggca cccctgtgct gttcctgcac ggtaacccga cctcctccta cgtgtggcgc
      181 aacatcatcc cgcatgttgc accgacccat cgcagcattg ctccagacct gatcggtatg
      241 ggcaaatccg acaaaccaga cctgggttat ttcttcgacg accacgtccg cttcatggat
      301 gccttcatcg aagccctggg tctggaagag gtcgtcctgg tcattcacga ctggggctcc
      361 gctctgggtt tccactgggc caagcgcaat ccagagcgcg tcaaaggtat tgcatttatg
      421 gagttcatcc gccctatccc gacctgggac ggcagtggca gcggtagcga tattccggct
      481 acatatgaat ttaccgatgg taaacattat atcaccaatg aaccgatacc gccgaaaggc
      541 agcggcagtg gtagcgaatg gccagaattt gcccgcgaga ccttccaggc cttccgcacc
      601 accgacgtcg gccgcaagct gatcatcgat cagaacgttt ttatcgaggg tacgctgccg
      661 atgggtgtcg tccgcccgct gactgaagtc gagatggacc attaccgcga gccgttcctg
      721 aatcctgttg accgcgagcc actgtggcgc ttcccaaacg agctgccaat cgccggtgag
      781 ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact ggctgcacca gtcccctgtc
      841 ccgaagctgc tgttctgggg caccccaggc gttctgatcc caccggccga agccgctcgc
      901 ctggccaaaa gcctgcctaa cagcaaggct gtggacatcg gcccgggtct gaatctgctg
      961 caagaagaca acccggacct gatcggcagc gagatcgcgc gctggctgtc tactctggag
     1021 atttccggtt aa
//
