LOCUS       sequence-333207-       11097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal aid-eDSR degron cassette with kanMX6 marker for S. pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11097)
  TITLE     Carr lab yeast expression plasmids
REFERENCE   2  (bases 1 to 11097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAW8-aid-eDSR-Turg1:kanMX6
FEATURES             Location/Qualifiers
     source          1..11097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(1451..1470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1570..1592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1630..1648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1821..1889,1890..2681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2039..2058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3341..3360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3594..3611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3764..3769,3770..3787,3788..3794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3807..3829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3826..3842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3874..3907)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3946..4635
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
     gene            5384..6740
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        5384..5727
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5728..6537
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5795..5814)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6543..6740
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6835..6852)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6835..6851)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6844..6866)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             9746..10840
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc gttgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg
     3901 aagttatgca tgctcggtgg gtcaggtgga agtggatctg gtgctatgat gggcagtgtc
     3961 gagctgaatc tgagggagac tgagctgtgt cttggtcttc ccggtggaga tacagtggct
     4021 ccggtaaccg gaaacaagag agggttctca gagacggttg atctgaagct aaatctgaat
     4081 aatgagcctg caaacaagga aggatctacg actcatgacg tcgtgacttt tgattccaag
     4141 gagaagagtg cttgtcctaa agatccagcc aaacctccgg ccaaggcaca agttgtggga
     4201 tggccaccgg tgagatcata ccggaagaac gtgatggttt cctgccaaaa atcaagcggt
     4261 ggcccggagg cggcggcgtt cgtgaaggta tcaatggacg gagcaccgta cttgaggaaa
     4321 atcgatttga ggatgtataa aagctacgat gagctttcta atgctttgtc caacatgttc
     4381 agctctttta ccatgggcaa acatggagga gaagaaggaa tgatagactt catgaatgag
     4441 aggaaattga tggatttggt gaatagctgg gactatgttc cctcttatga agacaaagac
     4501 ggtgattgga tgctcgtcgg cgacgttcct tggccaatgt tcgtcgatac atgcaagcgt
     4561 ttacgtctca tgaaaggatc ggatgccatt ggtctcgctc cgagggcgat ggagaagtgc
     4621 aagagcagag cttgagctag cactacgcca tatcatgccc atcccgctac ttctgctgct
     4681 aattaaactt taaacatctt tccagcattt acttgaacat caaactctaa tatcgaaatc
     4741 ttttcaaaca cttatcacat actcaaactt aaaccaacat taaacctgtt agacaaagcc
     4801 ccggggcttg cccatctgtt ttagacgtca cagaaggacc atgtttattt taaatttcac
     4861 atacatgtgt ttaattacct tcattgtata aattctcgaa actttctcca attcttcggt
     4921 cagataaaaa atttctctag tcaccgtctt ttatagagct tttgtcagat gtttttctgg
     4981 atggaatcat tgtccagtta ctatgctttt tatttataag tttgaaagtt tagttaactt
     5041 tttgttttct ttaatacatt ttttaatcat gtcaacattt gggatttgta gaagtagtaa
     5101 attttttgta catgttgtag aattggataa gaacgaaaac agctagtatg taaaaaaaac
     5161 gaaaatccca aattccgttg tttgtttgat atagtaatat ttatagcatt ttagtttcta
     5221 aagatttttg ataatctttt acatttgctt tcacatacat tcgcgctact accatacatc
     5281 atatcccgct ttctttaatt gcttccccac agactcaacc agcggggcct tcttctcgag
     5341 atctgtttag cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa
     5401 taccctcctt gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta
     5461 catttagccc atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac
     5521 ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc gagcagggaa acgctcccct
     5581 cacagacgcg ttgaattgtc cccacgccgc gcccctgtag agaaatataa aaggttagga
     5641 tttgccactg aggttcttct ttcatatact tccttttaaa atcttgctag gatacagttc
     5701 tcacatcaca tccgaacata aacaaccatg ggtaaggaaa agactcacgt ttcgaggccg
     5761 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     5821 gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt
     5881 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     5941 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     6001 gcatggttac tcaccactgc gatccccggc aaaacagcat tccaggtatt agaagaatat
     6061 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     6121 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     6181 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     6241 cctgttgaac aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc
     6301 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     6361 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     6421 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     6481 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     6541 gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata
     6601 ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca
     6661 tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt
     6721 gaatgctggt cgctatactg ctgtcgattc gatactaacg ccgccatcca gtcgacgaac
     6781 tagtataact tcgtatatgg tattatatac gaagttatgc ggccgcgcga attcactggc
     6841 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6901 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6961 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     7021 tctgtgcggt atttcacacc gcataccatc gtccggatca atgaagtaga tgagtttact
     7081 acctgtatat actttttttt ttgtcaaagt cacttttatg gcatttccat gttgaaggat
     7141 tagccacagc actaaatttt cagcgtatga ttgcttttaa atatttaatt ttcatcgttt
     7201 tttaattaat attcaaaacg atttaatgcc ttctacatct gaagtcaagc tcatagactc
     7261 gggttgggta gctaaaacta catctaacac gaaggggtat agccttctta aagtatactc
     7321 atgtataaac gttttgggtt catatttttg ggccacgtgg tttataaaaa ttcttaacta
     7381 caccactcgg tatcccgcac ccgtctacgt ttctacgatt tcgagctaat attgagtaaa
     7441 gtgattagca aaaaaaataa aagtactcgt tgtcggagat caagaatttt tctattatct
     7501 catctaaacc actttctaaa agcgaaaaac aaaatcgtaa tatgcagctt gaatgggctt
     7561 ccatagtttg aaagaaaaac cctagcagta ctggcaaggg agacattcct tttacctggc
     7621 taatcgccat cttccagcag gcgcaccatt gcccctgttt cactatccag gttacggata
     7681 tagttcatga caatatttac attggtccag ccaccagctt gcatgatctc cggtattgaa
     7741 actccagcgc gggccatatc tcgcgcggct ccgacacggg cactgtgtcc agaccaggcc
     7801 aggtatctct gaccagagtc atccttagcg ccgtaaatca atcgatgagt tgcttcaaaa
     7861 atcccttcca gggcgcgagt tgatagctgg ctggtggcag atggcgcggc aacaccattt
     7921 tttctgaccc ggcaaaacag gtagttattc ggatcatcag ctacaccaga gacggaaatc
     7981 catcgctcga ccagtttagt tacccccagg ctaagtgcct tctctacacc tgcggtgcta
     8041 accagcgttt tcgttctgcc aatatggatt aacattctcc caccgtcagt acgtgagata
     8101 tctttaaccc tgatcctggc aatttcggct atacgtaaca gggtgttata agcaatcccc
     8161 agaaatgcca gattacgtat atcctggcag cgatcgctat tttccatgag tgaacgaacc
     8221 tggtcgaaat cagtgcgttc gaacgctaga gcctgttttg cacgttcacc ggcatcaacg
     8281 ttttcttttc ggatccgccg cataaccagt gaaacagcat tgctgtcact tggtcgtggc
     8341 agcccggacc gacgatgaag catgtttagc tggcccaaat gttgctggat agtttttact
     8401 gccagaccgc gcgcctgaag atatagaaga taatcgcgaa catcttcagg ttctgcggga
     8461 aaccatttcc ggttattcaa cttgcaccat gccgcccacg accggcaaac ggacagaagc
     8521 attttccagg tatgctcaga aaacgcctgg cgatccctga acatgtccat caggttcttg
     8581 cgaacctcat cactcgttgc atcgaccggt aacttttaga ttaacatttg tttaaatatg
     8641 aaattaggca atgtaacata ctgcaggcaa attttggtgt acggtaagta aattggacat
     8701 atgatttaac aaagcgacta taagtcagaa agtgagaatg agattgaaat aattaattca
     8761 acttattcaa ttgatgatat gccaggattc ctcttccttt atcttcaatg tttcgattaa
     8821 caggtccatt atccggtttt tagtttcgct gcacattgcc gaatgacaat cggattcctt
     8881 taagctttgc ttttgccctg ttttctctcc ccagtaatcg ttgggtgact gaaccatcca
     8941 gtagccgaga gttacgtttt tcctgattta ataaatatcc atatctattt gtctcgtggt
     9001 gttttacagc aactaaccga aattactatt ttgcatcatc caaccataaa gagacaacaa
     9061 taattctaga aaacatataa caagtgtttt tttctattta cgtctcatac gcaaagtcca
     9121 ctttaccatg ttttggaaac ttgtacccaa ccattcattt ttcgattttg taatgaggct
     9181 cttttcttct aacaatcatt gaggtcatta tcatgtgtcc tcacctattg tttaaattga
     9241 ggcttgcgct attcccaaaa ttgtgggtat tattttcata tatcagtatc tgcaatttcg
     9301 gttcaactcc ggaagctcgg tacccaactg tgggaatact caggtatcgt aagatgcaag
     9361 agttcgaatc tcttagcaac cattattttt tttctctcaa cataacgaga acacacaggg
     9421 gcgctatcgc acagaatcaa attcgatgac tggaaatttt ttgttaattt cagaggtcgc
     9481 ctgacgcata tacctttttc aactgaaaaa ttgggagaaa aaggaaaggt gagagcgccg
     9541 gaaccggctt ttcatataga atagagaagc gttcatgact aaatgcttgc atcacaatac
     9601 ttgaagttga caatattatt taaggaccta ttgttttttc caataggtgg ttagcaatcg
     9661 tcttactttc taacttttct taccttttac atttcagcaa tatatatata tatatatata
     9721 tatatttcaa ggatatacca ttgtaatgtc tgcccctaag aagatcgtcg ttttgccagg
     9781 tgaccacgtt ggtcaagaaa tcacagccga agccattaag gttcttaaag ctatttctga
     9841 tgttcgttcc aatgtcaagt tcgatttcga aaatcattta attggtggtg ctgctatcga
     9901 tgctacaggt gttccacttc cagatgaggc gctggaagcc tccaagaagg ctgatgccgt
     9961 tttgttaggt gctgtgggtg gtcctaaatg gggtaccggt agtgttagac ctgaacaagg
    10021 tttactaaaa atccgtaaag aacttcaatt gtacgccaac ttaagaccat gtaactttgc
    10081 atccgactct cttttagact tatctccaat caagccacaa tttgctaaag gtactgactt
    10141 cgttgttgtt agagaattag tgggaggtat ttactttggt aagagaaagg aagacgatgg
    10201 tgatggtgtc gcttgggata gtgaacaata caccgttcca gaagtgcaaa gaatcacaag
    10261 aatggccgct ttcatggccc tacaacatga gccaccattg cctatttggt ccttggataa
    10321 agctaatgtt ttggcctctt caagattatg gagaaaaact gtggaggaaa ccatcaagaa
    10381 cgaatttcct acattgaaag ttcaacatca attgattgat tctgccgcca tgatcctagt
    10441 taagaaccca acccacctaa atggtattat aatcaccagc aacatgtttg gtgatatcat
    10501 ctccgatgaa gcctccgtta tcccaggctc cttgggtttg ttgccatctg cgtccttggc
    10561 ctctttgcca gacaagaaca ccgcatttgg tttgtacgaa ccatgccatg gttccgctcc
    10621 agatttgcca aagaataagg tcaaccctat cgccactatc ttgtctgctg caatgatgtt
    10681 gaaattgtca ttgaacttgc ctgaagaagg taaagccatt gaagatgcag ttaaaaaggt
    10741 tttggatgca ggtatcagaa ctggtgattt aggtggttcc aacagtacca ccgaagtcgg
    10801 tgatgctgtc gccgaagaag ttaagaaaat ccttgcttaa aaagattctc tttttttgtg
    10861 atatttgtac ataaacttta taaatgaaat tcataataga aacgacacga aattacaaaa
    10921 tggaatatgt tcatagggta gacgaaacta tatacgcaat ctacatacat ttatcaagaa
    10981 ggagaaaaag gaggatgtaa aggaatacag gtaagcaaat tgatactaat ggctcaacgt
    11041 gataaggaaa aagaattgca ctttaacatt aatattgaca aggaggaggg catcaca
//
