LOCUS       sequence-333279-        4325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expressed, chimeric transcription activator 
            comprising the AraC DNA binding domain and the XylS benzoate ligand 
            binding domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Picard HR, Schwingen KS, Green LM, Shis DL, Egan SM, Bennett MR, 
            Swint-Kruse L
  TITLE     Allosteric regulation within the highly interconnected structural 
            scaffold of AraC/XylS homologs tolerates a wide range of amino acid 
            changes.
  JOURNAL   Proteins. 2021 Aug 8. doi: 10.1002/prot.26206.
  PUBMED    34369028
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2021 Aug 8. doi: 10.1002/prot.26206."
COMMENT     Sequence Label: XXXXA-pHG165c
FEATURES             Location/Qualifiers
     source          1..4325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    92..232
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     147..166
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(247..264)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(418..1006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(498..517)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1177..1968,1969..2037))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1800..1819
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2038..2142)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2210..2228
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     2381..2403
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2395..2412
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2396..2412
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4124..4315
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(join(4309..4325,1..6))
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
       61 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
      121 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
      181 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
      241 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      301 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      361 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      421 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      481 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      541 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      601 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      661 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      721 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
      781 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
      841 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
      901 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
      961 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1021 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1081 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1141 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     1201 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     1261 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     1321 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     1381 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     1441 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     1501 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     1561 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     1621 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     1681 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     1741 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     1801 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     1861 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     1921 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     1981 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     2041 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     2101 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     2161 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     2221 cacgaggccc tttcgtcttc aagaatccgt taacggcatt cgccattcag gctgcgcaac
     2281 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     2341 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     2401 acgacggcca gtgccaagct tggctgcagg tcgacggatc cccgggaatt ccaccatcga
     2461 atggtgacac catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggggc
     2521 ccaagttcac ttaaaaagga gatcaacaat gaagcaattt tcgtactgaa acatcttaat
     2581 catgctgggg agggtttcta atggattttt gcctgctgaa cgaaaaaagc cagatttttg
     2641 tgcatgcgga accgtatgcg gtgagcgatt atgtgaacca gtatgtgggc acccatagca
     2701 ttcgcctgcc gaaaggcggc cgcccggcgg gccgcctgca tcatcgcatt tttggctgcc
     2761 tggatctgtg ccgcattagc tatggcggca gcgtgcgcgt gattagcccg ggcctggaaa
     2821 cctgctatca tctgcagatt attctgaaag gccattgcct gtggcgcggc catggccagg
     2881 aacattattt tgcgccgggc gaactgctgc tgctgaaccc ggatgatcag gcggatctga
     2941 cctatagcga agattgcgaa aaatttattg tgaaactgcc gagcgtggtg ctggatcgcg
     3001 cgtgcagcga taacaactgg cataaaccgc gcgaaggcat tcgctttgcg gcgcgccata
     3061 acctgcagca gctggatggc tttattaacc tgctgggcct ggtgtgcgat gaagcggaac
     3121 ataccaaaag catgccgcgc gtgcaggaac attatgcggg cattattgcg agcaaactgc
     3181 tggaaatgct gggcagcaac gtgagccgcg aaatttttag caaaggcaac ccggataatc
     3241 gggtacgcga ggcttgtcag tacatcagcg atcacctggc agacagcaat tttgatatcg
     3301 ccagcgtcgc acagcatgtt tgcttgtcgc cgtcgcgtct gtcacatctt ttccgccagc
     3361 agttagggat tagcgtctta agctggcgcg aggaccaacg tatcagccag gcgaagctgc
     3421 ttttgagcac cacccggatg cctatcgcca ccgtcggtcg caatgttggt tttgacgatc
     3481 aactctattt ctcgcgggta tttaaaaaat gcaccggggc cagcccgagc gagttccgtg
     3541 ccggttgtga agaaaaagtg aatgatgtag ccgtcaagtt gtcataatga gaattcgtaa
     3601 taatatagct ctagattaat tgcgcccgtt aacggcgggc agcgttgggt cctggccacg
     3661 ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac
     3721 tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac
     3781 gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga
     3841 aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg
     3901 gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat
     3961 ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa
     4021 ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat
     4081 cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa
     4141 aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact
     4201 caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga
     4261 tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     4321 gcagc
//
