LOCUS       sequence-33339-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration-free (episomal) expression of human OCT3/4 and shRNA 
            against p53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-hOCT3/4-shp53-F
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(190..207)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(441..460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttttgagact ataaatatcc cttggagaaa agccttgttt gggccgactc cagtggtaat
       61 ctacttcaag agagtagatt accactggag tcttttttgg aattcctgca gcccggggga
      121 tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
      181 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      241 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      301 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      361 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      421 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      481 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      541 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      601 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      661 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      721 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      781 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgc
//
