LOCUS       sequence-333414-        7296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPABPC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7296)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-DmPABPC1-V5His6_D
FEATURES             Location/Qualifiers
     source          1..7296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..39
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        103..2612
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2633..2698
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             4649..4690
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4700..4717
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4740..4757)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4790..4924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4814..4833)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4868..4887
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5525..6113)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5605..5624)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6284..7075,7076..7144))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6907..6926
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7145..7249)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattaa
       61 ttctatattc taaaaacaca aatgatactt ctaaaaaaaa atcatgaatg gcatcaactc
      121 tgaatcaaat ctttgcagat gcacctactt ctcatttcca ctgtcacatc atttttccag
      181 atctcgctgc ctgttatgtg gcccacaaac caagacacgt tttatggcca ttaaagctgg
      241 ctgatcgtcg ccaaacacca aatacataat gaatatgtac acattcgaga aagaagcgat
      301 caaagaagcg tcttcgggcg gagtaggaga atgcggagga gaaggagaac gagctgatct
      361 agtatctctc cacaatccaa tgccaactga ccaactggcc atattcggag caatttgaag
      421 ccaatttcca tcgcctggcg atcgctccat tcttggctat atgtttttca ccgttacccg
      481 gggccatttt caaagactcg tcggcaagat aagattgtgt cactcgctgt ctctcttcat
      541 ttgtcgaaga atgctgagga atttcgcgat gacgtcggcg agtattttga agaatgagaa
      601 taatttgtat ttatacgaaa atcagttagt ggaattttct acaaaaacat gttatctata
      661 gataattttg ttgcaaaata tgttgactat gacaaagatt gtatgtatat acctttaatg
      721 tattctcatt ttcttatgta tttataatgg caatgatgat actgatgata ttttaagatg
      781 atgccagacc aaaaggcttg aatttctgcg tcttttgccg aacgcagtgc atgtgcaatt
      841 gttgtttttt ggaatattca attttcggac tgtccgcttt gatttcagtt tcttggctta
      901 ttcaaaaagc aaagtaaagc caaaaaagcg agatggcaat accaaatgcg gcaaaacggt
      961 agtggaagga aaggggtgcg gggcagcgga aggaagggtg gggcggggcg tggcggggtc
     1021 tgtggctggg cgcgacgtca ccgacgttgg agccactcct ttgaccatgt gtgcgtgtgt
     1081 gtattattcg tgtctcgcca ctcgccggtt gtttttttct ttttatgctg cgctctctct
     1141 agcgccatct cgcttacgca tgctcaacgc accgcatgtt gccgtttcct tttatgcgtc
     1201 attttggctc gaaataggca attatttaaa caaagattag tcaacgaaaa cgctaaaata
     1261 aataagtcta caatatggtt acttattgcc atgtgtgtgc agccaacgat agcaacaaaa
     1321 gcaacaacac aggtggcttt ccctctttca ctttttgttt gcaagccgcg tgcgagcaag
     1381 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggcgaaaaa
     1441 catcaaggcg cctgatacga atgcatttgc aataacaatt gcgatattta atattgttta
     1501 tgaagctgtt tgacttcaaa acacacaaaa aaaaaaataa aacaaattat ttgaaagaga
     1561 attaggaatc ggacgcttat cgttagggta acaacaagaa atgcttactg agtcacagcc
     1621 tctggaaaac tgccgcaagc cagagagaga gagaaaaaga gggagagcag cttagaccgc
     1681 atgtgcttgt gtgtgaggcg tctctctctt cgtctctgtt gcgcaaacgc atagactgca
     1741 ctgaaaaaat cgattaccta ttttttatga atgaatattt gcactattac tattcaaaac
     1801 tattaagata gcaatcacat tcaatagcca aatactatac cacctgagcg atgcaacgaa
     1861 atgatcaatt tgagcaaaaa tgctgcatat ttaggacggc atcattatag aaatgcttct
     1921 tgctgtgtac ttttctctcg tctggcagct gtttcgccgt tattgttaaa accggcttaa
     1981 gttaggtgtg ttttctacga ctagtgaatg ccctactaga agatgtgtgt tgcacaaaat
     2041 gtccctggaa taaccaattt gaagtgcaga tagcagtaaa cgtaagctaa tatgaatatt
     2101 atttaactgt aatgttttaa tatcgctgga cattactaat aaacccacta taaacacatg
     2161 tacatatgta tgttttggca tacaatgagt agttggggaa aaaatgtgta aaagcaccgt
     2221 gaccatcaca gcataaagat aaccagctga agtatcgaat atgagtaacc cccaaattga
     2281 atcacatgcc gcaactgata ggacccatgg aagtacactc ttcatggcga tatacaagac
     2341 acacacaagc acgaacaccc agttgcggag gaaattctcc gtaaatgaaa acccaatcgg
     2401 cgaacaattc atacccatat atggtaaaag ttttgaacgc gacttgagag cggagagcat
     2461 tgcggctgat aaggttttag cgctaagcgg gctttataaa acgggctgcg ggaccagttt
     2521 tcatatcact accgtttgag ttcttgtgct gtgtggatac tcctcccgac acaaagccgc
     2581 tccatcagcc agcagtcgtc taatccagag accccggatc ggggtaccca acatggacgc
     2641 acaaacacga cgacgtgagc gtcgcgctga gaaacaagct caatggaaag ctgcaaacgg
     2701 tggatctcct ccaagcttga tggcttctct atacgtcggt gatctcccac aggacgtcaa
     2761 cgaatcggga ctttttgaca agttctcgtc tgctggtcca gtgctgtcca ttcgtgtctg
     2821 ccgcgatgtg attacccgtc gctcgttggg ctatgcctac gtcaacttcc agcagccagc
     2881 cgatgctgag cgtgctttgg acaccatgaa ctttgacctg gttcgcaaca agcccattcg
     2941 cattatgtgg tctcagcgtg atccgtctct tcgtcgctcc ggtgtgggca acgtgttcat
     3001 caagaactta gatagggcca tcgacaacaa ggcaatctac gacactttct ccgccttcgg
     3061 caacatttta agctgcaagg tagccaccga tgaaaagggc aactcaaagg gctatggatt
     3121 cgtccacttc gagaccgagg aggccgcaaa cacgtccatc gacaaagtca atggcatgtt
     3181 gcttaacggc aagaaggtct acgtgggtaa gttcatcccg cgcaaggagc gcgagaagga
     3241 gctgggcgag aaggctaagc tcttcaccaa tgtatatgtg aagaacttca ctgaggattt
     3301 cgacgatgaa aaactgaagg aattctttga gccctacggc aagataacca gctacaaggt
     3361 catgtccaag gaggatggca agagcaaggg cttcggattc gttgcattcg agacaacaga
     3421 ggctgctgag gcggccgttc aggctctcaa tggcaaggac atgggagagg gcaagtccct
     3481 gtacgtggct cgtgcccaga agaaggccga acgccagcag gagctgaagc gcaagttcga
     3541 ggaactgaag cagaagcgtc acgagtcagt gttcggcgtt aacttgtacg ttaagaacct
     3601 ggacgacaca atcgatgacg atcgtctgcg catcgcgttc tctccatacg gcaacatcac
     3661 atcggccaag gtcatgaccg atgaggaggg tcgctccaag ggattcggct tcgtgtgctt
     3721 caatgccgca agcgaggcta cctgcgccgt caccgagctg aacggtcgcg tcgtcggcag
     3781 taagccgttg tacgttgctt tggcccaaag gaaggaggag cgcaaggctc atctcgcctc
     3841 gcagtacatg cgtcacatga ccggcatgcg tatgcagcag ctgggacaga tctaccagcc
     3901 caacgcggct agcggcttct ttgtgccgac ccttccgtca aatcagcgtt tcttcggttc
     3961 ccaggtggcc actcagatga gaaacacacc ccgctgggtg ccccaggtgc gtcccccagc
     4021 agccatacag ggtgtccagg ccggagctgc cgctgctggt ggcttccagg gcacagccgg
     4081 tgcggtgccc actcagttcc gttcggctgc tgctggcgct cgcggggcac agcctcaggt
     4141 gcagggcaca cacgccgccg ctgctgcggc taacaacatg cgcaacactg gagcacgcgc
     4201 tattactggc cagcaaactg cagcacccaa catgcagatc cccggagccc aaatcgctgg
     4261 cggtgcccag cagcgtactt ccaactataa gtacacctca aacatgcgta atcctccagt
     4321 gccacagttg catcagaccc agccaattcc acagcaattg caaggaaaga attctgagaa
     4381 gctcattgcc tcgttgctgg ccaacgccaa gccgcaggag cagaaacaga tcctgggcga
     4441 gcgtctgtat ccgatgattg aacatatgca cgccaatttg gctggtaaga tcaccggcat
     4501 gttgttggag atcgaaaact ctgagctctt gcacatgatc gaggatcagg aggcccttaa
     4561 ggccaaggtg gaggaggctg tggccgtgct tcaggtgcac cgcgtcaccg agcccgccaa
     4621 ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
     4681 cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc
     4741 ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg atccagacat
     4801 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     4861 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     4921 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     4981 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agtcgaccga
     5041 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
     5101 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
     5161 tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc
     5221 ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca
     5281 aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg
     5341 tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt
     5401 ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc
     5461 atcagggaca gcttcaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5521 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5581 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5641 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5701 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5761 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5821 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5881 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5941 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     6001 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6061 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6121 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6181 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6241 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6301 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6361 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6421 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6481 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6541 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     6601 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6661 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6721 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6781 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6841 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6901 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6961 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7021 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7081 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7141 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7201 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7261 gaaaagtgcc acctgacgtc taagaaacca ttatta
//
