LOCUS       sequence-333499-        1392 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pGEX2T-TUBG1 bacterial expression plasmid for production of 
            recombinant GST fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Corvaisier M, Zhou J, Malycheva D, Cornella N, Chioureas D, 
            Gustafsson NMS, Rossello CA, Ayora S, Li T, Ekstrom-Holka K, 
            Jirstrom K, Lindstrom L, Alvarado-Kristensson M
  TITLE     The gamma-tubulin meshwork assists in the recruitment of PCNA to 
            chromatin in mammalian cells.
  JOURNAL   Commun Biol. 2021 Jun 22;4(1):767. doi: 10.1038/s42003-021-02280-1.
  PUBMED    34158617
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-06-22"; volume: "4(1):767. doi"; pages: " 
            10.1038/s42003-021-02280-1"
COMMENT     Sequence Label: pGEX2T-TUBG1
FEATURES             Location/Qualifiers
     source          1..1392
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccaaaatcgg atctggttcc gcgtggatcc caagctatgc cgagggaaat catcacccta
       61 cagttgggcc agtgcggcaa tcagattggg ttcgagttct ggaaacagct gtgcgccgag
      121 catggtatca gccccgaggg catcgtggag gagttcgcca ccgagggcac tgaccacaag
      181 gacgtctttt tctaccaggc agacgatgag cactacatcc cccgggccgt gctgctggac
      241 ttggaacccc gggtgatcca ctccatcctc aactccccct atgccaagct ctacaaccca
      301 gagaacatct acctgtcgga acatggagga ggagctggca acaactgggc cagcggattc
      361 tcccagggag aaaagatcca tgaggacatt tttgacatca tagaccggga ggcagatggt
      421 agtgacagtc tagagggctt tgtgctgtgt cactccattg ctggggggac aggctctgga
      481 ctgggttcct acctcttaga acggctgaat gacaggtatc ctaagaagct ggtgcagaca
      541 tactcagtgt ttcccaacca ggacgagatg agcgatgtgg tggtccagcc ttacaattca
      601 ctcctcacac tcaagaggct gacgcagaat gcagactgtg tggtggtgct ggacaacaca
      661 gccctgaacc ggattgccac agaccgcctg cacatccaga acccatcctt ctcccagatc
      721 aaccagctgg tgtctaccat catgtcagcc agcaccacca ccctgcgcta ccctggctac
      781 atgaacaatg acctcatcgg cctcatcgcc tcgctcattc ccaccccacg gctccacttc
      841 ctcatgaccg gctacacccc tctcactacg gaccagtcag tggccagcgt gaggaagacc
      901 acggtcctgg atgtcatgag gcggctgctg cagcccaaga acgtgatggt gtccacaggc
      961 cgagaccgcc agaccaacca ctgctacatc gccatcctca acatcatcca gggagaggtg
     1021 gaccccaccc aggtccacaa gagcttgcag aggatccggg aacgcaagtt ggccaacttc
     1081 atcccgtggg gccccgccag catccaggtg gccctgtcga ggaagtctcc ctacctgccc
     1141 tcggcccacc gggtcagcgg gctcatgatg gccaaccaca ccagcatctc ctcgctcttc
     1201 gagagaacct gtcgccagta tgacaagctg cgtaagcggg aggccttcct ggagcagttc
     1261 cgcaaggagg acatgttcaa ggacaacttt gatgagatgg acacatccag ggagattgtg
     1321 cagcagctca tcgatgagta ccatgcggcc acacggccag actacatctc ctggggcacc
     1381 caggagcagt ga
//
