LOCUS       sequence-333613-       20605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre-dependent Cas9 expression plasmid and targeting vector for the 
            mouse Rosa26 locus. Cas9 expression is Cre-dependent and the 
            targeting vector has positive (Neo) and negative (DTA) selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20605)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 20605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: LSL-Cas9-Rosa26TV
FEATURES             Location/Qualifiers
     source          1..20605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..1085
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     enhancer        1181..1484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1486..1763
                     /label=chicken beta-actin promoter
     intron          1764..2780
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2704..2726
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2788..2807
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2856..2889
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    2913..2946
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3113..3247
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3367..3501
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3621..3755
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3784..3817
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3875..3884
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3884..3949
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3956..3976
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4001..8101
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4001..8101
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8102..8149
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8159..8215
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(8216..8218,8219..8932)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8216..8218,8219..8929)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8519..8538)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8866..8887
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8983..9571
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9036..9056)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9454..9465)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9574..9590)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    9598..9805
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    9813..9846
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        9868..10367
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     protein_bind    10387..10420
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      10421..10430
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10427..11230
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10490..10509)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11100..11119
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11240..11701
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     misc_feature    11767..16107
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        16157..16656
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             16683..17255
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          17332..17397
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    17471..17695
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(17757..17777)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(17757..17775)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(17796..17812)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17796..17812)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(17809..17831)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    17820..17836
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(17844..17850,17851..17868,17869..17874))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17889..17910
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(18027..18044)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(18198..18786)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18278..18297)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(18957..19748,19749..19817))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     19580..19599
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(19818..19922)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      19949..20404
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(20036..20055)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     20246..20267
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     20530..20552
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     20544..20561
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     20545..20561
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     20571..20590
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20571..20589
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggcagcgg aggagcgctt ccggccgacg tctcgtcgct gattggcttc ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctgg acgtgcgggc gcggtcggcc tctggcgggg cgggggrggg
      421 gagggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
      481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
      541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
      601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
      661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
      721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
      781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
      841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
      901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
      961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
     1021 cgccctgggc ctgggagaat cccttccccc tcttccctcg tgatctgcaa ctccagtctt
     1081 tctagcctta attaaccgtt taaacaattc tgcaggaatc tagttattaa tagtaatcaa
     1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1441 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     1501 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     1561 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccrggsg
     1621 gggsggggsg gggsgrgggg sggggsgggg sgaggcggag aggtgcggcg gcagccaatc
     1681 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     1741 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
     1801 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
     1861 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
     1921 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg
     1981 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg
     2041 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg
     2101 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga
     2161 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg
     2221 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
     2281 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
     2341 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc
     2401 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
     2461 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
     2521 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
     2581 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     2641 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
     2701 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
     2761 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     2821 ttggcaaaga attgatttga taccgcgggc cctaagaagt tcctattctc tagaaagtat
     2881 aggaacttcg tcgacattta aatcatttaa atataacttc gtataatgta tgctatacga
     2941 agttattcgc gatgaataaa tgaaagcttg cagatctgcg actctagagg atctgcgact
     3001 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     3061 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     3121 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3181 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     3241 ctggatctgc gactctagag gatcataatc agccatacca catttgtaga ggttttactt
     3301 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     3361 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3421 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3481 gtatcttatc atgtctggat ctgcgactct agaggatcat aatcagccat accacatttg
     3541 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     3601 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     3661 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3721 ccaaactcat caatgtatct tatcatgtct ggatccccat caagctgatc cggaaccctt
     3781 aatataactt cgtataatgt atgctatacg aagttattag gtccctcgac ctgcagccca
     3841 agctagatcg aattcggccg gccttcgaac acgtgccacc atggactata aggaccacga
     3901 cggagactac aaggatcatg atattgatta caaagacgat gacgataaga tggccccaaa
     3961 gaagaagcgg aaggtcggta tccacggagt cccagcagcc gacaagaagt acagcatcgg
     4021 cctggacatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc
     4081 cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat
     4141 cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc
     4201 cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa
     4261 cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga
     4321 agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta
     4381 ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa
     4441 ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt
     4501 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct
     4561 ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc
     4621 caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca
     4681 gctgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga gcctgggcct
     4741 gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa
     4801 ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga
     4861 cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt
     4921 gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca
     4981 ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa
     5041 agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg gcggagccag
     5101 ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga
     5161 actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg
     5221 cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga
     5281 tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat
     5341 cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa
     5401 gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgcttccgc
     5461 ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct
     5521 gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga ccaaagtgaa
     5581 atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat
     5641 cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta
     5701 cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa
     5761 cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga
     5821 caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga
     5881 cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat
     5941 gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa
     6001 cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt
     6061 cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat
     6121 ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc
     6181 cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt
     6241 gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca
     6301 gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat
     6361 caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa
     6421 cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact
     6481 ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga gctttctgaa
     6541 ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg gcaagagcga
     6601 caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa
     6661 cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct
     6721 gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac
     6781 aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa
     6841 gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa
     6901 ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta
     6961 cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt
     7021 cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga
     7081 aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac
     7141 cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga
     7201 aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag
     7261 catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga
     7321 gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc
     7381 taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa
     7441 agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat
     7501 catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa
     7561 agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa
     7621 cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg aactggccct
     7681 gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc
     7741 ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga
     7801 gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga
     7861 caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa
     7921 tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca agtactttga
     7981 caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg ccaccctgat
     8041 ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggcga
     8101 caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg gaagcggagc
     8161 cactaacttc tccctgttga aacaagcagg ggatgtcgaa gagaatcccg ggccagtgag
     8221 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     8281 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     8341 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     8401 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     8461 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     8521 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     8581 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     8641 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     8701 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     8761 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     8821 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     8881 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaacgcgtat
     8941 gcatggccgg ccctgcagga attcgatatc aagcttatcg ataatcaacc tctggattac
     9001 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     9061 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     9121 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     9181 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     9241 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     9301 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     9361 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     9421 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     9481 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     9541 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg acctcgactg
     9601 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     9661 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     9721 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     9781 aagacaatgg caggcatgct ggggaactag tggtgccagg gcgtgccctt gggctccccg
     9841 ggcgcggcgg ccgcatcgaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     9901 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     9961 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
    10021 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
    10081 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
    10141 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
    10201 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
    10261 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
    10321 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcaatcgcc
    10381 gctagcgaag ttcctattct ctagaaagta taggaacttc gccaccatgg gatcggccat
    10441 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
    10501 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
    10561 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
    10621 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
    10681 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
    10741 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
    10801 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
    10861 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
    10921 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
    10981 tgatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
    11041 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
    11101 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
    11161 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
    11221 gttcttctga ggggatccgc tgtaagtctg cagaaattga tgatctatta aacaataaag
    11281 atgtccacta aaatggaagt ttttcctgtc atactttgtt aagaagggtg agaacagagt
    11341 acctacattt tgaatggaag gattggagct acgggggtgg gggtggggtg ggattagata
    11401 aatgcctgct ctttactgaa ggctctttac tattgcttta tgataatgtt tcatagttgg
    11461 atatcataat ttaaacaagc aaaaccaaat taagggccag ctcattcctc ccactcatga
    11521 tctatagatc tatagatctc tcgtgggatc attgtttttc tcttgattcc cactttgtgg
    11581 ttctaagtac tgtggtttcc aaatgtgtca gtttcatagc ctgaagaacg agatcagcag
    11641 cctctgttcc acatacactt cattctcagt attgttttgc caagttctaa ttccatcaga
    11701 aagcttgcag aagatctccc caactggggt aacctttgag ttctctcagt tgggggggcg
    11761 cgccggctag aagatgggcg ggagtcttct gggcaggctt aaaggctaac ctggtgtgtg
    11821 ggcgttgtcc tgcaggggaa ttgaacaggt gtaaaattgg agggacaaga cttcccacag
    11881 attttcggtt ttgtcgggaa gttttttaat aggggcaaat aaggaaaatg ggaggatagg
    11941 tagtcatctg gggttttatg cagcaaaact acaggttatt attgcttgtg atccgcctcg
    12001 gagtattttc catcgaggta gattaaagac atgctcaccc gagttttata ctctcctgct
    12061 tgagatcctt actacagtat gaaattacag tgtcgcgagt tagactatgt aagcagaatt
    12121 ttaatcattt ttaaagagcc cagtacttca tatccatttc tcccgctcct tctgcagcct
    12181 tatcaaaagg tattttagaa cactcatttt agccccattt tcatttatta tactggctta
    12241 tccaacccct agacagagca ttggcatttt ccctttcctg atcttagaag tctgatgact
    12301 catgaaacca gacagattag ttacatacac cacaaatcga ggctgtagct ggggcctcaa
    12361 cactgcagtt cttttataac tccttagtac actttttgtt gatcctttgc cttgatcctt
    12421 aattttcagt gtctatcacc tctcccgtca ggtggtgttc cacatttggg cctattctca
    12481 gtccagggag ttttacaaca atagatgtat tgagaatcca acctaaagct taactttcca
    12541 ctcccatgaa tgcctctctc ctttttctcc atttataaac tgagctatta accattaatg
    12601 gtttccaggt ggatgtctcc tcccccaata ttacctgatg tatcttacat attgccaggc
    12661 tgatatttta agacattaaa aggtatattt cattattgag ccacatggta ttgattactg
    12721 cttactaaaa ttttgtcatt gtacacatct gtaaaaggtg gttccttttg gaatgcaaag
    12781 ttcaggtgtt tgttgtcttt cctgacctaa ggtcttgtga gcttgtattt tttctattta
    12841 agcagtgctt tctcttggac tggcttgact catggcattc tacacgttat tgctggtcta
    12901 aatgtgattt tgccaagctt cttcaggacc tataattttg cttgacttgt agccaaacac
    12961 aagtaaaatg attaagcaac aaatgtattt gtgaagcttg gtttttaggt tgttgtgttg
    13021 tgtgtgcttg tgctctataa taatactatc caggggctgg agaggtggct cggagttcaa
    13081 gagcacagac tgctcttcca gaagtcctga gttcaattcc cagcaaccac atggtggctc
    13141 acaaccatct gtaatgggat ctgatgccct cttctggtgt gtctgaagac cacaagtgta
    13201 ttcacattaa ataaataaat cctccttctt cttctttttt ttttttttaa agagaatact
    13261 gtctccagta gaatttactg aagtaatgaa atactttgtg tttgttccaa tatggtagcc
    13321 aataatcaaa ttactcttta agcactggaa atgttaccaa ggaactaatt tttatttgaa
    13381 gtgtaactgt ggacagagga gccataactg cagacttgtg ggatacagaa gaccaatgca
    13441 gactttaatg tcttttctct tacactaagc aataaagaaa taaaaattga acttctagta
    13501 tcctatttgt ttaaactgct agctttactt aacttttgtg cttcatctat acaaagctga
    13561 aagctaagtc tgcagccatt actaaacatg aaagcaagta atgataattt tggatttcaa
    13621 aaatgtaggg ccagagttta gccagccagt ggtggtgctt gcctttatgc ctttaatccc
    13681 agcactctgg aggcagagac aggcagatct ctgagtttga gcccagcctg gtctacacat
    13741 caagttctat ctaggatagc caggaataca cacagaaacc ctgttgggga ggggggctct
    13801 gagatttcat aaaattataa ttgaagcatt ccctaatgag ccactatgga tgtggctaaa
    13861 tccgtctacc tttctgatga gatttgggta ttattttttc tgtctctgct gttggttggg
    13921 tcttttgaca ctgtgggctt tctttaaagc ctccttcctg ccatgtggtc tcttgtttgc
    13981 tactaacttc ccatggctta aatggcatgg ctttttgcct tctaagggca gctgctgaga
    14041 tttgcagcct gatttccagg gtggggttgg gaaatctttc aaacactaaa attgtccttt
    14101 aatttttttt ttaaaaaatg ggttatataa taaacctcat aaaatagtta tgaggagtga
    14161 ggtggactaa tattaaatga gtccctcccc tataaaagag ctattaaggc tttttgtctt
    14221 atacttaact ttttttttaa atgtggtatc tttagaacca agggtcttag agttttagta
    14281 tacagaaact gttgcatcgc ttaatcagat tttctagttt caaatccaga gaatccaaat
    14341 tcttcacagc caaagtcaaa ttaagaattt ctgactttta atgttaattt gcttactgtg
    14401 aatataaaaa tgatagcttt tcctgaggca gggtctcact atgtatctct gcctgatctg
    14461 caacaagata tgtagactaa agttctgcct gcttttgtct cctgaatact aaggttaaaa
    14521 tgtagtaata cttttggaac ttgcaggtca gattctttta taggggacac actaagggag
    14581 cttgggtgat agttggtaaa atgtgtttca agtgatgaaa acttgaatta ttatcaccgc
    14641 aacctacttt ttaaaaaaaa aagccaggcc tgttagagca tgcttaaggg atccctagga
    14701 cttgctgagc acacaagagt agttacttgg caggctcctg gtgagagcat atttcaaaaa
    14761 acaaggcaga caaccaagaa actacagtta aggttacctg tctttaaacc atctgcatat
    14821 acacagggat attaaaatat tccaaataat atttcattca agttttcccc catcaaattg
    14881 ggacatggat ttctccggtg aataggcaga gttggaaact aaacaaatgt tggttttgtg
    14941 atttgtgaaa ttgttttcaa gtgatagtta aagcccatga gatacagaac aaagctgcta
    15001 tttcgaggtc tcttggttta tactcagaag cacttctttg ggtttccctg cactatcctg
    15061 atcatgtgct aggcctacct taggctgatt gttgttcaaa taaacttaag tttcctgtca
    15121 ggtgatgtca tatgatttca tatatcaagg caaaacatgt tatatatgtt aaacatttgt
    15181 acttaatgtg aaagttaggt ctttgtgggt ttgattttta attttcaaaa cctgagctaa
    15241 ataagtcatt tttacatgtc ttacatttgg tggaattgta taattgtggt ttgcaggcaa
    15301 gactctctga cctagtaacc ctacctatag agcactttgc tgggtcacaa gtctaggagt
    15361 caagcatttc accttgaagt tgagacgttt tgttagtgta tactagttta tatgttggag
    15421 gacatgttta tccagaagat attcaggact atttttgact gggctaagga attgattctg
    15481 attagcactg ttagtgagca ttgagtggcc tttaggcttg aattggagtc acttgtatat
    15541 ctcaaataat gctggccttt tttaaaaagc ccttgttctt tatcaccctg ttttctacat
    15601 aatttttgtt caaagaaata cttgtttgga tctccttttg acaacaatag catgttttca
    15661 agccatattt tttttccttt tttttttttt ttttggtttt tcgagacagg gtttctctgt
    15721 atagccctgg ctgtcctgga actcactttg tagaccaggc tggcctcgaa ctcagaaatc
    15781 cgcctgcctc tgcctcctga gtgccgggat taaaggcgtg caccaccacg cctggctaag
    15841 ttggatattt tgttatataa ctataaccaa tactaactcc actgggtgga tttttaattc
    15901 agtcagtagt cttaagtggt ctttattggc ccttcattaa aatctactgt tcactctaac
    15961 agaggctgtt ggtactagtg gcacttaagc aacttcctac ggatatacta gcagattaag
    16021 ggtcagggat agaaactagt ctagcgtttt gtatacctac cagctttata ctaccttgtt
    16081 ctgatagaaa tatttcagga catctagctt atcgataccg tcgacggtat cgataagctt
    16141 gatatcgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
    16201 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
    16261 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
    16321 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
    16381 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
    16441 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
    16501 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
    16561 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
    16621 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcaggtcctc gccatggatc
    16681 ctgatgatgt tgttgattct tctaaatctt ttgtgatgga aaacttttct tcgtaccacg
    16741 ggactaaacc tggttatgta gattccattc aaaaaggtat acaaaagcca aaatctggta
    16801 cacaaggaaa ttatgacgat gattggaaag ggttttatag taccgacaat aaatacgacg
    16861 ctgcgggata ctctgtagat aatgaaaacc cgctctctgg aaaagctgga ggcgtggtca
    16921 aagtgacgta tccaggactg acgaaggttc tcgcactaaa agtggataat gccgaaacta
    16981 ttaagaaaga gttaggttta agtctcactg aaccgttgat ggagcaagtc ggaacggaag
    17041 agtttatcaa aaggttcggt gatggtgctt cgcgtgtagt gctcagcctt cccttcgctg
    17101 aggggagttc tagcgttgaa tatattaata actgggaaca ggcgaaagcg ttaagcgtag
    17161 aacttgagat taattttgaa acccgtggaa aacgtggcca agatgcgatg tatgagtata
    17221 tggctcaagc ctgtgcagga aatcgtgtca ggcgatctct ttgtgaagga accttacttc
    17281 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
    17341 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
    17401 ccaacctatg gaactgatga atgggagcag tggtggaatg cagatcctag agctcgctga
    17461 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
    17521 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
    17581 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
    17641 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
    17701 gaggcggaaa gaaccagctg gggctcgagg gggggcccgg tacccagctt ttgttccctt
    17761 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
    17821 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    17881 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
    17941 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    18001 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    18061 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    18121 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    18181 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    18241 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    18301 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    18361 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    18421 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    18481 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    18541 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    18601 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    18661 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    18721 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    18781 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    18841 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    18901 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    18961 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    19021 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    19081 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    19141 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    19201 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    19261 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    19321 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    19381 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    19441 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    19501 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    19561 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    19621 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    19681 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    19741 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    19801 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    19861 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    19921 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
    19981 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
    20041 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    20101 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    20161 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    20221 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    20281 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    20341 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
    20401 atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    20461 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    20521 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
    20581 cactataggg cgaattggag ctcca
//
