LOCUS       sequence-333629-        2524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sRNA part (C).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Greco FV, Pandi A, Erb TJ, Grierson CS, Gorochowski TE
  TITLE     Harnessing the central dogma for stringent multi-level control of 
            gene expression.
  JOURNAL   Nat Commun. 2021 Mar 19;12(1):1738. doi: 10.1038/s41467-021-21995-7.
  PUBMED    33741937
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-19"; volume: "12(1):1738. doi"; pages: " 
            10.1038/s41467-021-21995-7"
COMMENT     Sequence Label: pC-01
FEATURES             Location/Qualifiers
     source          1..2524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     161..180
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     353..370
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(698..717)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(699..717)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(728..745)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(728..744)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(737..759)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        1060..1164
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1165..1233,1234..2025)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAG*EQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1383..1402)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(2196..2524,1..260)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
       61 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      121 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      181 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      241 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
      301 gctggccttt tgctcattag gcaccccagg ctttacccga acgaccgagc gcagcgagtc
      361 agtgagcgag gaagcggaga gcgcccaata cgcaaggaaa cagctatgac catgttaatg
      421 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcggaagg cccatgaggc
      481 cagtcttgtg ctccaggtac caacgtcccc atccaactca accaagatgg gtacaacgcc
      541 tgtaaacagc tcctctcctt tactggtctc aaagctctat aagtgcaaat gcttgttcta
      601 tcgtcttgtt cacctattgg aaacgggtcc aataggtccc accttgagac cgagctctgg
      661 acaagacacg tggccttgac ggccttccgc caattcgccc tatagtgagt cgtattacgt
      721 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      781 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
      841 ccgaaacgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggagcg ccctgtagcg
      901 gccactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
      961 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     1021 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1081 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1141 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1201 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1261 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1321 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1381 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggt aagagcaact cggtcgccgc
     1441 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     1501 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1561 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     1621 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     1681 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     1741 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     1801 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     1861 tctggagccg gtgagcgtgg ttctcgcggt atcattgcag cactggggcc agatggtaag
     1921 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     1981 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2041 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2101 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2161 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2221 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2281 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2341 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2401 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2461 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2521 ggtt
//
