LOCUS       sequence-333652-        2524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Toehold switch part (G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Greco FV, Pandi A, Erb TJ, Grierson CS, Gorochowski TE
  TITLE     Harnessing the central dogma for stringent multi-level control of 
            gene expression.
  JOURNAL   Nat Commun. 2021 Mar 19;12(1):1738. doi: 10.1038/s41467-021-21995-7.
  PUBMED    33741937
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-19"; volume: "12(1):1738. doi"; pages: " 
            10.1038/s41467-021-21995-7"
COMMENT     Sequence Label: pG-01
FEATURES             Location/Qualifiers
     source          1..2524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(36..624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(116..135)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(795..1586,1587..1655))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1418..1437
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1656..1760)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2061..2083
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2075..2092
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2076..2092
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2103..2122
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2103..2121
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2450..2467)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
       61 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      121 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      181 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      241 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      301 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      361 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      421 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      481 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      541 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      601 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      661 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      721 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
      781 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
      841 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
      901 ttaccatctg gccccagtgc tgcaatgata ccgcgagaac cacgctcacc ggctccagat
      961 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1021 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1081 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1141 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1201 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1261 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1321 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1381 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1441 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1501 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1561 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1621 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1681 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1741 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     1801 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     1861 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtggcc
     1921 gctacagggc gctcccattc gccattcagg ctgcgcaact gttgggaagg gcgtttcggt
     1981 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     2041 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcga
     2101 cgtaatacga ctcactatag ggcgaattgg cggaaggccg tcaaggccac gtgtcttgtc
     2161 cagagctcgg tctcacatca cgcttgttct catgtcttgt ctcctctgtt tcaagacgat
     2221 agaacaagca tttgcactta ttacttgaga ccagtaaagg agaggagctg tttacaggcg
     2281 ttgtacccat cttggttgag ttggatgggg acgttaatgg tacctggagc acaagactgg
     2341 cctcatgggc cttccgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     2401 attaacatgg tcatagctgt ttccttgcgt attgggcgct ctccgcttcc tcgctcactg
     2461 actcgctgcg ctcggtcgtt cgggtaaagc ctggggtgcc taatgagcaa aaggccagca
     2521 aaag
//
