LOCUS       sequence-333690-        9235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Eepresses SLC7A11 (also named xCT) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Koppula P, Zhang Y, Shi J, Li W, Gan B
  TITLE     The glutamate/cystine antiporter SLC7A11/xCT enhances cancer cell 
            dependency on glucose by exporting glutamate.
  JOURNAL   J Biol Chem. 2017 Aug 25;292(34):14240-14249. doi: 
            10.1074/jbc.M117.798405. Epub 2017 Jun 19.
  PUBMED    28630042
REFERENCE   2  (bases 1 to 9235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M117.798405"; journalName: "J Biol Chem"; date: 
            "2017-08-25- 25"; volume: "292"; issue: "34"; pages: "14240-14249"
COMMENT     Sequence Label: plenti6-SLC7A11/xCT-V5
FEATURES             Location/Qualifiers
     source          1..9235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1692
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1715..2018
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2019..2222
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2173..2193
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2219..2243
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2339..2363
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3867..3891)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3944..3985
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4009..4597
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4062..4082)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4480..4491)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4612..4928
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4779..4914
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4976..5023
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5042..5440
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5527..5760
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5832..5953
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5869..5888)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5923..5942
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5993..6128
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6148..6167)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6149..6167)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6177..6194)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6177..6193)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6186..6208)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6335..6790
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6422..6441)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6632..6653
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6816..6920
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6921..6989,6990..7781)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7139..7158)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7952..8540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8441..8460
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8694..8711
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8828..8849
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8864..8869,8870..8887,8888..8894)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8902..8918
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8907..8929
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8926..8942
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8926..8942
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8961..8981
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8963..8981
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9009..9235
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 attcaaaatt ttatcgataa gcttgggagt tccgcgttac ataacttacg gtaaatggcc
     1741 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     1801 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     1861 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     1921 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     1981 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     2041 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     2101 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     2161 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     2221 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2281 gaagacaccg actctagagg atccactagt ccagtgtggt ggaattctgc agatatcaac
     2341 aagtttgtac aaaaaagttg gcatggtcag aaagcctgtt gtgtccacca tctccaaagg
     2401 aggttacctg cagggaaatg ttaacgggag gctgccttcc ctgggcaaca aggagccacc
     2461 tgggcaggag aaagtgcagc tgaagaggaa agtcacttta ctgaggggag tctccattat
     2521 cattggcacc atcattggag caggaatctt catctctcct aagggcgtgc tccagaacac
     2581 gggcagcgtg ggcatgtctc tgaccatctg gacggtgtgt ggggtcctgt cactatttgg
     2641 agctttgtct tatgctgaat tgggaacaac tataaagaaa tctggaggtc attacacata
     2701 tattttggaa gtctttggtc cattaccagc ttttgtacga gtctgggtgg aactcctcat
     2761 aatacgccct gcagctactg ctgtgatatc cctggcattt ggacgctaca ttctggaacc
     2821 attttttatt caatgtgaaa tccctgaact tgcgatcaag ctcattacag ctgtgggcat
     2881 aactgtagtg atggtcctaa atagcatgag tgtcagctgg agcgcccgga tccagatttt
     2941 cttaaccttt tgcaagctca cagcaattct gataattata gtccctggag ttatgcagct
     3001 aattaaaggt caaacgcaga actttaaaga cgccttttca ggaagagatt caagtattac
     3061 gcggttgcca ctggcttttt attatggaat gtatgcatat gctggctggt tttacctcaa
     3121 ctttgttact gaagaagtag aaaaccctga aaaaaccatt ccccttgcaa tatgtatatc
     3181 catggccatt gtcaccattg gctatgtgct gacaaatgtg gcctacttta cgaccattaa
     3241 tgctgaggag ctgctgcttt caaatgcagt ggcagtgacc ttttctgagc ggctactggg
     3301 aaatttctca ttagcagttc cgatctttgt tgccctctcc tgctttggct ccatgaacgg
     3361 tggtgtgttt gctgtctcca ggttattcta tgttgcgtct cgagagggtc accttccaga
     3421 aatcctctcc atgattcatg tccgcaagca cactcctcta ccagctgtta ttgttttgca
     3481 ccctttgaca atgataatgc tcttctctgg agacctcgac agtcttttga atttcctcag
     3541 ttttgccagg tggcttttta ttgggctggc agttgctggg ctgatttatc ttcgatacaa
     3601 atgcccagat atgcatcgtc ctttcaaggt gccactgttc atcccagctt tgttttcctt
     3661 cacatgcctc ttcatggttg ccctttccct ctattcggac ccatttagta cagggattgg
     3721 cttcgtcatc actctgactg gagtccctgc gtattatctc tttattatat gggacaagaa
     3781 acccaggtgg tttagaataa tgtcagagaa aataaccaga acattacaaa taatactgga
     3841 agttgtacca gaagaagata agttatgccc aactttcttg tacaaagtgg ttgatatcca
     3901 gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa
     3961 ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa tgatcgacaa tcaacctctg
     4021 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     4081 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     4141 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     4201 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     4261 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     4321 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4381 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     4441 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4501 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4561 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gcgatggtac cggtgtggaa
     4621 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4681 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4741 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4801 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4861 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4921 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     4981 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     5041 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     5101 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     5161 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     5221 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     5281 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     5341 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     5401 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcacaattcg agctcggtac
     5461 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     5521 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     5581 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5641 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5701 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5761 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     5821 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5881 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5941 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     6001 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6061 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     6121 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     6181 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6241 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6301 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     6361 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6421 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6481 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6541 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6601 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6661 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6721 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     6781 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6841 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6901 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6961 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7021 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7081 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7141 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7201 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7261 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7321 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7381 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7441 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7501 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7561 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7621 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7681 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7741 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7801 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7861 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7921 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7981 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8041 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     8101 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8161 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8221 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8281 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8341 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8401 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8461 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8521 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8581 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8641 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8701 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     8761 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     8821 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     8881 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     8941 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     9001 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     9061 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     9121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     9181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaac
//
