LOCUS       sequence-333700-        3225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genomic fragment containing the upstream region and coding region of
            DET3/VHA-C cloned into pDONR221.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Shi L, Dean GH, Zheng H, Meents MJ, Haslam TM, Haughn GW, Kunst L
  TITLE     ECERIFERUM11/C-TERMINAL DOMAIN PHOSPHATASE-LIKE2 Affects Secretory 
            Trafficking.
  JOURNAL   Plant Physiol. 2019 Nov;181(3):901-915. doi: 10.1104/pp.19.00722. 
            Epub 2019 Sep 4.
  PUBMED    31484679
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.19.00722"; journalName: "Plant Physiol"; date: 
            "2019-11"; volume: "181"; issue: "3"; pages: "901-915"
COMMENT     Sequence Label: VHACg/pDONR221
FEATURES             Location/Qualifiers
     source          1..3225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..29
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3197..3221)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggggacaagt ttgtacaaaa aagcaggctt tagatgaatc aatcttctta cttcagagac
       61 tcgcgatttt ggtttgagga cgacgattca gagttagggc tttgaagctg aatcaaaaca
      121 ctcgagccaa tttagtttgt aaccctagaa gtttttgtag ggttttaccg tctctgtttt
      181 ttattatttg gatctctgaa cacatttggc ccagtattag cccatttacc tatcttctca
      241 aaatatttgg ttcttaacaa attcaagcct aaccattagt acaattttaa ttttgatctg
      301 tacattttca aaaaccggac tcgaaccaag atcaaattca acaattgtgg caattttaat
      361 ggtcgagtct ccagtgtgta tgtggcagta ggagaacgtg ccgggggata ttaaaccggc
      421 tcaatgctac agtcgtaccg gtttgttttt ttactcgttg gtatataatt aaaaccggtt
      481 atccaatttg acaggcagag agaagaagat ttgattgaag tacagatcca tcggagaaga
      541 agacagagag attgaagatc aatgacttcg agatattggg tggtgtctct tccagtgaaa
      601 gactctgctt cttcgttatg gaatcgatta caagagcaga tctctaagca ttcctttgat
      661 actccagttt atcgggtact ctgatttttg ctcgatttgg tcttattgca aaatttagca
      721 cagatcttat actatctctt gctactttcg tgtctacgat tctaattacg atctaattcc
      781 gtgagttttg gtttcaattt cgcagtttaa cattcctaat cttcgtgttg gaaccttaga
      841 ttctctactc gctctcggcg atgatctgct taaggtaaaa ttcatttgat cactgagtgg
      901 attttatgtt tttgatttta aaattcctct cttgattgag aaatttttga gatttgtagt
      961 cgaacagctt tgtggaagga gtttcgcaaa agatcaggag acagatcgaa gaattggaga
     1021 ggatttctgg tgttgagagc aacgctctta cagttgatgg agttcctgtt gattcttatc
     1081 tcaccaggta tttgtgattt tccgtctcag aaggagttaa ttgctaattt ggtggtgatc
     1141 gaattgttaa agaacgaaac ttttgttgat tggtggactc aaggtttgtg tgggatgaag
     1201 ctaagtaccc aacaatgtca cctctgaagg aggttgtgga caatattcag tctcaggttg
     1261 caaaaattga ggatgatctc aaggtattgt agcttaaacc ttttgtttta ttgtaaaaaa
     1321 aaaggaagct tcacctttaa gcagaaagat tagctgcatg agaccgatgg tataaaattt
     1381 gcaattcgag tttcttacga tgatcaaatg aattgctttg gttcaaatgg tggtgttaac
     1441 gactctgatt gcacatttta ttcaggttcg tgttgctgaa tataacaata tccgtggtca
     1501 actcaatgcc attaaccgaa agcaaagtgg aaggtaattc ttttcttctc atatttatac
     1561 tataagagca cactagaaac tgtgggcggt ttagcaacaa caaattttga agtttgtttt
     1621 ctcatctatc tgatttttgc ttgaagcatg atttaccacg tgaacgtttt agaataatac
     1681 aaagttattt tccagaatat tgctgttggt ctttatcgat atctcagctt tgtttagtat
     1741 ctttgtggca ttgtggctga tcgtcctcct ttttgtctca gcttagctgt tcgtgacctc
     1801 tcaaacttgg ttaagccaga agatattgtc gagtccgaac atctcgtaac tctccttgca
     1861 gttgttccga agtattccca aaaagactgg ctagcatgtt atgagacttt gaccgattat
     1921 gtggtaaatt tgttgacttg ctaggtttat tgaatagacc agtggtaaca ctactggttt
     1981 gacgtaccgt gattttaatc aatttatcct aacttgtttc aggttcctag gtcctcgaag
     2041 aaattgtttg aggataatga gtatgccctt tacaccgtca ctctctttac tcgtgtcgca
     2101 gataatttca ggattgctgc tcgtgagaaa ggattccaag tgagtattct gttcaagttc
     2161 ctgatattac ctctagagtt acgtagaagt taaaaatgat tacatgctag taggagtgaa
     2221 atttcctacg aactgattgt attcatggtg caagttcaga aatttggttt agattcaaag
     2281 atttgttcat catatattgt acaccatcct cttctaggtc cgtgattttg aacaaagtgt
     2341 tgaagcacaa gagactcgta aacaagagct agcaaagttg gttcaggatc aggaaagttt
     2401 gagaagctct cttttgcagt ggtgctacac cagttatgga gaggtaggta tctacacatt
     2461 gctctgaata gatattctct tgtaatacaa tacctgaatc tgttttgatc ttcggtctac
     2521 atctgtaggt tttcagctcc tggatgcatt tctgtgctgt gcgtacattc gctgagagca
     2581 ttatgagata cggtttacct ccggcgttct tggtaaatta ggactccaag ctatgctatt
     2641 ttagagaatt cctctaccct actatctccc ttaccaaatt actttctctt gtctttccag
     2701 gcatgtgtct tatctccggc tgtgaaaagt gaaaagaaag tacgctccat tcttgaacgc
     2761 ttgtgtgatt ctaccaacag gtatgttaaa actctctacc tatctatcgc tctaggtgta
     2821 ggatacatgg aagctgttta acatcaacct tcatattgat ctggcgaaga aaaatgtact
     2881 gatagttatg accttaataa cgaaagaaat tgcagaagtt tgaggagaaa gtcaggaacc
     2941 aagtaaaacc aaacttgtat tagcatgcaa tgcgttcttg aggctaaatc tattaatcag
     3001 aactaagtcg ctttagttag attcatctat tgatgtagtg tagttcgatg agatttcaaa
     3061 ttgagcgttt tgggtctcat tgtatgaaaa caatgcagtt tatactggaa aagcgaggag
     3121 gatgcaggag ccatggctgg tttagctggt gactcagaga cacatcctta tgtctccttc
     3181 actatcaacc ttgctcaccc agctttcttg tacaaagtgg tcccc
//
