LOCUS       sequence-33394-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCS2 Flag Smad3 EPSM A208S
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             125..148
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcac
      121 catggactac aaggacgacg atgacaagtc gtccatcctg cctttcactc ccccgatcgt
      181 gaagcgcctg ctgggctgga agaagggcga gcagaacggg caggaggaga aatggtgcga
      241 gaaggcggtc aagagcctgg tcaagaaact caagaagacg gggcagctgg acgagctgga
      301 gaaggccatc accacgcaga acgtcaacac caagtgcatc accatcccca ggtccctgga
      361 tggccggttg caggtgtccc atcggaaggg gctccctcat gtcatctact gccgcctgtg
      421 gcgatggcca gacctgcaca gccaccacga gctgcgggcc atggagctgt gtgagttcgc
      481 cttcaatatg aagaaggacg aggtctgcgt gaatccctac cactaccaga gagtagagac
      541 accagttcta cctcctgtgt tggtgccacg ccacacagag atcccggccg agttcccccc
      601 actggacgac tacagccatt ccatccccga aaacactaac ttccccgcag gcatcgagcc
      661 ccagagcaat attccagagg tccctccccc tggctacctg agtgaagatg gagaaaccag
      721 tgaccaccag atgaaccaca gcatggacgc aggtgctcca aacctatccc cgaatccgat
      781 ggccccagca cataataact tggacctgca gccagttacc tactgcgagc cggccttctg
      841 gtgctccatc tcctactacg agctgaacca gcgcgtc
//
