LOCUS       sequence-334166-        7841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses in Candida albicans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Van Genechten W, Demuyser L, Dedecker P, Van Dijck P
  TITLE     Presenting a codon-optimized palette of fluorescent proteins for use
            in Candida albicans.
  JOURNAL   Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.1038/s41598-020-63308-w.
  PUBMED    32273559
REFERENCE   2  (bases 1 to 7841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-04-9"; volume: "10(1):6158. doi"; pages: " 
            10.1038/s41598-020-63308-w"
COMMENT     Sequence Label: CIp10 - ffDronpa ATGme
FEATURES             Location/Qualifiers
     source          1..7841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(701..717)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(702..718)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(1127..1145)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1166..1182)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1166..1182)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1179..1201)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1190..1206
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1214..1220,1221..1238,1239..1244))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1259..1280
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1397..1414)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1568..2156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1648..1667)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2327..3118,3119..3187))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2950..2969
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3188..3292)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3319..3774
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3406..3425)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3616..3637
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3900..3922
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3914..3931
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3915..3931
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3938..3957
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3938..3956
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgctgcaga tgtcagttat taaaccagat atgaaaatta aattaagaat ggaaggtgct
       61 gttaatggtc atccatttgc tattgaaggt gttggtttag gtaaaccatt tgaaggtaaa
      121 caatcaatgg atttaaaagt taaagaaggt ggtccattac catttgctta tgatatttta
      181 actactgctt tttgttatgg taatagagtt tttgctaaat atccagaaaa tattgttgat
      241 tattttaaac aatcatttcc agaaggttat tcatgggaaa gatcaatgaa ttatgaagat
      301 ggtggtattt gtaatgctac taatgatatt actttagatg gtgattgtta tatttatgaa
      361 attagatttg atggtgttaa ttttccagct aatggtccag ttatgcaaaa aagaactgtt
      421 aaatgggaac catcaactga aaaattatat gttagagatg gtgttttaaa aggtgatgtt
      481 aatatggctt tatcattaga aggtggtggt cattatagat gtgattttaa aactacttat
      541 aaagctaaaa aagttgttca attaccagat tatcattttg ttgatcatca tattgaaatt
      601 aaatcacatg ataaagatta ttcaaatgtt aatttacatg aacatgctga agctcattca
      661 gaattaccaa gacaagctaa ataactagta gcgacgtcat cgataccgtc gacctcgagg
      721 agtgaaattc tggaaatctg gaaatctggt tttgtattct tgttattctt ctttttgtta
      781 ttacatatat aacttgttac ttttttaaaa aaatctttgt ttattttata aatatataaa
      841 actaaattta agaaaaagag aaaaatgttt tatttgagag attgaaattt tacttgaatt
      901 tagcttagct tttataaagt attattatgt aaaaaaacaa aacaaatata cattaaaaag
      961 ttaagactat aaaatagcca cccaaggcat ttctatatct tgttgttgtt gttttcatct
     1021 tctgtatcag aggaacttat tttattattt tcgtcacggg tattttctct tgtttgatga
     1081 ttcatcccat tcattccatc aaaaatgtcg gtaccagctt ttgttccctt tagtgagggt
     1141 taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1201 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1261 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     1321 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     1381 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1441 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1501 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1561 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1621 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1681 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1741 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1801 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1861 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1921 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1981 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     2041 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2101 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2161 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2221 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2281 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2341 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2401 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2461 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2521 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2581 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2641 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2701 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2761 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2821 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2881 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2941 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3001 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3061 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3121 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3181 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3241 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3301 cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3361 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     3421 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     3481 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     3541 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     3601 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     3661 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     3721 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc
     3781 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     3841 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     3901 ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga
     3961 attggagctc caccgcggtg gcggccgcgt ttataataaa cttagtctaa tatttctttt
     4021 ggaccaaaat agaaactgtt ctcaggtggc aaaaaacagc ttaaagtctg tgttaaaaaa
     4081 agatctcggc tatgccaatc aaaaaggagt gacattatta tgtaaaagaa gaaatgttgt
     4141 aattccgcga aacaacaatt ttaatgctta ctgtgccgcc accaatatcc tatggccaag
     4201 gggcctttaa acatgccacg gaaatataca gtcaaagtaa acctactatg tctttagagc
     4261 cttcaggatc aattgttttt aaatgaatga ctgcaaaact agaaaagtag cctcttacat
     4321 aagcaagtca tttgtacacc gagagtttag taaccagtga agcaagtata tctggcaaac
     4381 ctgaaactta tctgtgaatt ataaagctca taagtaaaaa gtgtagaaaa gaagataagg
     4441 cagaccaaat acaaatttgc aacagtacaa cgaatatttc agcgtcaaaa ctagagaata
     4501 ataaagaaaa cgatcttttc aaaaagaaaa aaccttttag ttttcctttg ttgttgttgt
     4561 gggtgtgtgc tatttatatt atatagttta ctcataatac cataaaatat tcggtttgat
     4621 taggttattt taataagcta atttgtttct aatcgtgtaa tttatgctgt gtatattaag
     4681 tagtgtgtgc actgcccaaa aatgtttgtt gtttatagtc ggttaaagag aaaaaagaaa
     4741 aaaagatcca tacacacacg ttaattagtt gttcaacgta atacactcat attttgttct
     4801 tatttgcttt cggtcgctgt tctcaccaag atttattgcc aacgaaacaa ttttttttta
     4861 tatattttca gatttttctt tttttccttt cctttccttt tctaattttc actcctggtt
     4921 ttctttcttt cttagaaaca ttatctcgat attaatatta aaaaaatata atcattcaaa
     4981 ctccggaatg tctactactt tggatgatac tgcttataga tacagaactt ctgttccagg
     5041 tgatgctgaa gctattgaag ctttggatgg ttctttcact accgatactg tttttagagt
     5101 tactgctact ggtgatggtt tcactttgag agaagttcca gttgatccac cattgactaa
     5161 ggttttccca gatgatgaat ccgatgatga atccgatgct ggtgaagatg gtgatccaga
     5221 ttctagaact ttcgttgctt atggtgatga tggtgatttg gctggtttcg ttgttgtttc
     5281 ttattctggt tggaacagaa gattgactgt tgaagatatt gaagttgctc cagaacatag
     5341 aggtcatggt gttggtagag ctttgatggg tttggctact gaattcgcca gagaaagagg
     5401 tgctggtcat ttgtggttgg aagttaccaa tgttaatgct ccagctattc atgcttatag
     5461 aagaatgggt ttcactttgt gtggtttgga tactgcttta tacgatggta ctgcttccga
     5521 tggtgaacaa gctttgtata tgtctatgcc atgtccataa taactcgggg ggatccgagt
     5581 gaaattctgg aaatctggaa atctggtttt gtattcttgt tattcttctt tttgttatta
     5641 catatataac ttgttacttt tttaaaaaaa tctttgttta ttttataaat atataaaact
     5701 aaatttaaga aaaagagaaa aatgttttat ttgagagatt gaaattttac ttgaatttag
     5761 cttagctttt ataaagtatt attatgtaaa aaaacaaaac aaatatacat taaaaagtta
     5821 agactataaa atagccaccc aaggcatttc tatatcttgt tgttgttgtt ttcatcttct
     5881 gtatcagagg aacttatttt attattttcg tcacgggtat tttctcttgt ttgatgattc
     5941 atcccattct tccatcataa aatactagtg gatcccccag atccttatcc ttaaacagat
     6001 tctaaaacaa catctttgaa accagaagaa actttcttac ctttttcttc ggttgaacct
     6061 tcaccgtgca aagccaataa tgaacccaag tcgaattttg gttgtttcaa caatttgact
     6121 tttctgatgt ggacattttg taatgggaaa atggtttggg tggatttttc aatttcacgg
     6181 ccaatgactt ctggaatcaa tttggaagtt aattgagcta aagtacagtt ggaaacttct
     6241 ctttgcataa tttcaatcat cttctttctg acttctctca atttagagga ttgagcgtaa
     6301 gtagttttct tgatttggtt tggttgtctt ttggtgaaag caatggcaaa aactctcaaa
     6361 acgtaatcgt cggaagtttt aacagtgaca ttagcttcaa ctaatgattg ccattttctg
     6421 accaatgatc ttaatttgtc agaagtgaaa tccaaaccat gaggcctcca tgaggcctcc
     6481 atgaggcctc catgaggcct ccatggaggc ctcatggaag ttggtcaaca agtttttacc
     6541 ttggacttca tcaactctca atttgatttt tctgtaagag tggtcttcgg aaccttgtaa
     6601 gtcggccaaa caaacttcga aaactctacc tttcaagcca tcagcggcat tctttaaacc
     6661 ggtagatctg ttgatcaaag ttttaccaac atttctgttt tcaaaagtgg ttggagcttt
     6721 gatgtcaaac caatcttttc tggtgaatgg gtcaacgacc ttctttttta atcctttctt
     6781 tcctttggac aatctcttgt ttttaccgac agccatgttg tacttgagtt ggatctacgc
     6841 gtcagcctcg tttataataa acttagtcta atatttcttt tggaccaaaa tagaaactgt
     6901 tctcaggtgg caaaaaacag cttaaagtct gtgttaaaaa aagatctcgg ctatgccaat
     6961 caaaaaggag tgacattatt atgtaaaaga agaaatgttg taattccgcg aaacaacaat
     7021 tttaatgctt actgtgccgc caccaatatc ctatggccaa ggggccttta aacatgccac
     7081 ggaaatatac agtcaaagta aacctactat gtctttagag ccttcaggat caattgtttt
     7141 taaatgaatg actgcaaaac tagaaaagta gcctcttaca taagcaagtc atttgtacac
     7201 cgagagttta gtaaccagtg aagcaagtat atctggcaaa cctgaaactt atctgtgaat
     7261 tataaagctc ataagtaaaa agtgtagaaa agaagataag gcagaccaaa tacaaatttg
     7321 caacagtaca acgaatattt cagcgtcaaa actagagaat aataaagaaa acgatctttt
     7381 caaaaagaaa aaacctttta gttttccttt gttgttgttg tgggtgtgtg ctatttatat
     7441 tatatagttt actcataata ccataaaata ttcggtttga ttaggttatt ttaataagct
     7501 aatttgtttc taatcgtgta atttatgctg tgtatattaa gtagtgtgtg cactgcccaa
     7561 aaatgtttgt tgtttatagt cggttaaaga gaaaaaagaa aaaaagatcc atacacacac
     7621 gttaattagt tgttcaacgt aatacactca tattttgttc ttatttgctt tcggtcgctg
     7681 ttctcaccaa gatttattgc caacgaaaca attttttttt atatattttc agatttttct
     7741 ttttttcctt tcctttcctt ttctaatttt cactcctggt tttctttctt tcttagaaac
     7801 attatctcga tattaatatt aaaaaaatat aatcattcaa a
//
