LOCUS       sequence-33436-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Samara NL, Datta AB, Berndsen CE, Zhang X, Yao T, Cohen RE, 
            Wolberger C
  TITLE     Structural insights into the assembly and function of the SAGA 
            deubiquitinating module.
  JOURNAL   Science. 2010 May 21. 328(5981):1025-9.
  PUBMED    20395473
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2010 May 21. 328(5981):1025-9."
COMMENT     Sequence Label: Sgf73(1-106)/PET32a
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(388..408)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(418..432)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(448..492)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(499..516)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(526..543)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(565..891)
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     RBS             899..921
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cttccttcgg gcttgttagc agccggaatc tcagtggtgg tggtggtggt gctcgagtgc
       61 ggccgcttag ctactttttc cagatgctcc cgcacaatgg ttttctaagt ggtctacaat
      121 tgctgtcaaa gctaagggtt tcccacattt ttcgcacact ctatactgaa tcggtttatc
      181 caaaggatct tcaatgagtt gtgcattcgg attgaagtat ttttttagac tttcgcgatt
      241 catgtgatca tactgcaaag gagtgtcctt cgcggacgac attaaacttt tccatgaatc
      301 gttggaagga ccacttccct gagataaaga atattcctca ataactttgg gcttaattcc
      361 tttaatctct gcatcgcctg acctcattcc ctgaaaatac aggttttcag ccatggcctt
      421 gtcgtcgtcg tcggtaccca gatctgggct gtccatgtgc tggcgttcga atttagcagc
      481 agcggtttct ttcataccag aaccgcgtgg caccagacca gaagaatgat gatgatgatg
      541 gtgcatatgg ccagaaccag aaccggccag gttagcgtcg aggaactctt tcaactgacc
      601 tttagacagt gcacccactt tggttgccgc cacttcaccg tttttgaaca gcagcagagt
      661 cgggatacca cggatgccat atttcggcgc agtgccaggg ttttgatcga tgttcagttt
      721 tgcaacggtc agtttgccct gatattcgtc agcgatttca tccagaatcg gggcgatcat
      781 tttgcacgga ccgcaccact ctgcccagaa atcgacgagg atcgccccgt ccgctttgag
      841 tacatccgtg tcaaaactgt cgtcagtcag gtgaataatt ttatcgctca tatgtatatc
      901 tccttcttaa agttaaacaa aatatca
//
