LOCUS       sequence-334403-       11291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Expression of EF1a.SLC6A1.WT.EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11291)
  TITLE     Rarebase PBC plasmids
REFERENCE   2  (bases 1 to 11291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MS2392.EF1a.SLC6A1.WT.EGFP.P2A.BLAST.JL.p164
FEATURES             Location/Qualifiers
     source          1..11291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        173..552
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        554..752
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     707..727
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             770..950
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    997..1122
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1615..1848
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2033..2077
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2375..2492
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2585..2796
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2834..2843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2840..2842,2843..2845,2846..3556)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2885..2906)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3146..3165)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3493..3514
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5453..5509
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5510..5908
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    5939..6527
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5992..6012)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6410..6421)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6530..6546)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6531..6547)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7052..7232
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7258..7275)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7264..7488
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7534..7962
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7621..7640)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7831..7852
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7971..7991)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8136..8270
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8197..8216
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8332..8379
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8398..8772
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8902..9023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8939..8958)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8993..9012
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9072..9088)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9072..9088)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9085..9107)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9096..9112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9120..9126,9127..9144,9145..9150))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9165..9186
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9303..9320)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9474..10062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9554..9573)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10233..11024,11025..11093))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10856..10875
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11094..11198)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(11273..11291,1))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
       61 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
      121 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
      181 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      241 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      301 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      361 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      421 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      481 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      541 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      601 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      661 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      721 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg
      781 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      841 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      901 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      961 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     1021 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1081 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1141 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1201 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1261 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1321 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1381 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1441 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     1501 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1561 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1621 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1681 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1741 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1801 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1861 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1921 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1981 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2041 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2101 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2161 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2221 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2281 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2341 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2401 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2461 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2521 cagtttggtt aattagctag ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt
     2581 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     2641 tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     2701 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     2761 gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctagac gtacgctaga
     2821 ggatcgaacc cttgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     2881 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ctggcgaggg
     2941 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     3001 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     3061 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     3121 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     3181 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     3241 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     3301 ggccgacaag cagaagaacg gcatcaaggc gaacttcaag atccgccaca acatcgagga
     3361 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     3421 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     3481 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     3541 ggacgagctg tacaagggtg gaggaggttc tggaggcggt ggaagtggtg gcggaggtag
     3601 cccacctcct ccaagacctc cgccaccttc accaccgcct ccatcgatgg cgaccaacgg
     3661 cagcaaggtg gccgacgggc agatctccac cgaggtcagc gaggcccctg tggccaatga
     3721 caagcccaaa accttggtgg tcaaggtgca gaagaaggcg gcagacctcc ccgaccggga
     3781 cacgtggaag ggccgcttcg acttcctcat gtcctgtgtg ggctatgcca tcggcctggg
     3841 caacgtctgg aggttcccct atctctgcgg gaaaaatggt gggggagcct tcctgatccc
     3901 ctatttcctg acactcatct ttgcgggggt cccactcttc ctgctggagt gctccctggg
     3961 ccagtacacc tccatcgggg ggctaggggt atggaagctg gctcctatgt tcaagggcgt
     4021 gggccttgcg gctgctgtgc tatcattctg gctgaacatc tactacatcg tcatcatctc
     4081 ctgggccatt tactacctgt acaactcctt caccacgaca ctgccgtgga aacagtgcga
     4141 caacccctgg aacacagacc gctgcttctc caactacagc atggtcaaca ctaccaacat
     4201 gaccagcgct gtggtggagt tctgggagcg caacatgcat cagatgacgg acgggctgga
     4261 taagccaggt cagatccgct ggccactggc catcacgctg gccatcgcct ggatccttgt
     4321 gtatttctgt atctggaagg gtgttggctg gactggaaag gtggtctact tttcagccac
     4381 atacccctac atcatgctga tcatcctgtt cttccgtgga gtgacgctgc ccggggccaa
     4441 ggagggcatc ctcttctaca tcacacccaa cttccgcaag ctgtctgact ccgaggtgtg
     4501 gctggatgcg gcaacccaga tcttcttctc atacgggctg ggcctggggt ccctgatcgc
     4561 tctcgggagc tacaactctt tccacaacaa tgtctacagg gactccatca tcgtctgctg
     4621 catcaattcg tgcaccagca tgttcgcagg attcgtcatc ttctccatcg tgggcttcat
     4681 ggcccatgtc accaagaggt ccattgctga tgtggcggcc tcaggccccg ggctggcgtt
     4741 cctggcatac ccagaggcgg tgacccagct gcctatctcc ccactctggg ccatcctctt
     4801 cttctccatg ctgttgatgc tgggcattga cagccagttc tgcactgtgg agggcttcat
     4861 cacagccctg gtggatgagt accccaggct cctccgcaac cgcagagagc tcttcattgc
     4921 tgctgtctgc atcatctcct acctgatcgg tctctctaac atcactcagg ggggtattta
     4981 tgtcttcaaa ctctttgact actactctgc cagtggcatg agcctgctgt tcctcgtgtt
     5041 ctttgaatgt gtctctattt cctggtttta cggtgtcaac cgattctatg acaatatcca
     5101 agagatggtt ggatccaggc cctgcatctg gtggaaactc tgctggtctt tcttcacacc
     5161 aatcattgtg gcgggcgtgt tcattttcag tgctgtgcag atgacgccac tcaccatggg
     5221 aaactatgtt ttccccaagt ggggccaggg tgtgggctgg ctgatggctc tgtcttccat
     5281 ggtcctcatc cccgggtaca tggcctacat gttcctcacc ttaaagggct ccctgaagca
     5341 gcgcatccaa gtcatggtcc agcccagcga agacatcgtt cgcccagaga atggtcctga
     5401 gcagccccag gcgggcagct ccaccagcaa ggaggcctac atcggatctg gtgccacgaa
     5461 tttctcgcta ctcaagcagg ccggtgatgt cgaggaaaac cctggtccta tggccaagcc
     5521 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
     5581 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
     5641 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
     5701 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
     5761 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
     5821 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
     5881 gccctctggt tatgtgtggg agggctaaga attcgaattc gatatcaagc ttatcggtaa
     5941 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6001 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6061 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6121 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6181 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6241 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6301 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6361 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6421 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6481 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     6541 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     6601 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     6661 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6721 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     6781 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     6841 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     6901 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     6961 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     7021 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     7081 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7141 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7201 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     7261 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     7321 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     7381 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     7441 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     7501 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     7561 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     7621 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     7681 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     7741 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7801 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7861 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     7921 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     7981 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8041 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8101 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccagttc
     8161 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8221 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8281 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     8341 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     8401 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     8461 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     8521 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     8581 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     8641 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     8701 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     8761 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     8821 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     8881 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     8941 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9001 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     9061 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9121 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9181 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     9241 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     9301 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9361 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9421 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9481 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9541 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9601 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9661 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9721 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9781 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9841 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9901 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9961 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10021 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10081 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    10141 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    10201 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    10261 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    10321 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10381 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10441 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10501 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10561 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10621 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10681 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10741 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10801 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10861 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10921 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10981 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    11041 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    11101 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    11161 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    11221 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
    11281 gctctgatgc c
//
