LOCUS       sequence-33443-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Radoshevich L, Murrow L, Chen N, Fernandez E, Roy S, Fung C, Debnath
            J
  TITLE     ATG12 conjugation to ATG3 regulates mitochondrial homeostasis and 
            cell death.
  JOURNAL   Cell. 2010 Aug 20. 142(4):590-600.
  PUBMED    20723759
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Aug 20. 142(4):590-600."
COMMENT     Sequence Label: pMSCV FHA-ATG12
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..63
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             76..102
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             76..102
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(615..638)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gaattagatc tctcgagatc ttccgctcga tgaaccatgg actacaagga cgacgatgac
       61 aagctcgatg gaggataccc ctacgacgtg cccgactacg ccggaggact cgagcggatg
      121 tcggaagatt cagaggttgt gctgcagctc ccctcggctc ctgtaggagc cggcggcgag
      181 agccttccag agctctcccc ggaaacagcc accccagagc ccccgtcctc ggctgcagtt
      241 tcgcccggaa cggaggaacc tcccggagac accaagaaaa aaattgacat cctgctgaag
      301 gctgtaggag acactcctat aatgaaaaca aagaaatggg ctgtggagcg aacccggacc
      361 atccaaggac tcattgactt catcaaaaag ttccttaaac tggtggcctc ggaacagttg
      421 tttatttatg tgaatcagtc ctttgcccct tccccagacc aagaanttgg aactctatat
      481 gagtgttttg gcagtgatgg taaactggtc ctgcattact gcaaatccca ggcatgggga
      541 tgaatagttt agcggcctcg aggttaacga attctanngg gtaggggagg cgcttttccc
      601 aanggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
      661 gcctctggnc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
      721 ggtggcccct tcgcgccanc ttctactcct cccctagtca ggaagttccc ccccgccccg
      781 cagctcgcgt cgtgcaggac gtgacaatgg aaagta
//
