LOCUS       sequence-334436-         338 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with 
            Broccoli RNA aptamer..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Gonzales DT, Yandrapalli N, Robinson T, Zechner C, Tang TD
  TITLE     Cell-Free Gene Expression Dynamics in Synthetic Cell Populations.
  JOURNAL   ACS Synth Biol. 2022 Jan 21;11(1):205-215. doi: 
            10.1021/acssynbio.1c00376. Epub 2022 Jan 4.
  PUBMED    35057626
REFERENCE   2  (bases 1 to 338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00376"; journalName: "ACS Synth Biol"; date: 
            "2022-01-21- 21"; volume: "11"; issue: "1"; pages: "205-215"
COMMENT     Sequence Label: pEXP5-NT/6xHis mCherry F30 Broccoli
FEATURES             Location/Qualifiers
     source          1..338
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(205..223)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      219..266
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(311..329)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgccactcca ccggcggcat ggacgagctg tacaagggca gctaaagggt gatccggttg
       61 ccatgtgtat gtgggagacg gtcgggtcca gatattcgta tctgtcgagt agagtgtggg
      121 ctcccacata ctctgatgat ccttcgggat cattcatggc aactgctaac aaagcccgaa
      181 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      241 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga taattcttga
      301 agacgaaagg gcctcgtgat acgcctattt ttataggt
//
