LOCUS       sequence-334443-         376 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd 
            Broccoli RNA aptamer..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Gonzales DT, Yandrapalli N, Robinson T, Zechner C, Tang TD
  TITLE     Cell-Free Gene Expression Dynamics in Synthetic Cell Populations.
  JOURNAL   ACS Synth Biol. 2022 Jan 21;11(1):205-215. doi: 
            10.1021/acssynbio.1c00376. Epub 2022 Jan 4.
  PUBMED    35057626
REFERENCE   2  (bases 1 to 376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00376"; journalName: "ACS Synth Biol"; date: 
            "2022-01-21- 21"; volume: "11"; issue: "1"; pages: "205-215"
COMMENT     Sequence Label: pEXP5-NT/6xHis mCherry F30 2xdBroccoli
FEATURES             Location/Qualifiers
     source          1..376
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
       61 tacaagggca gctaaagggt gatcttgcca tgtgtatgtg ggagacggtc gggtccatct
      121 gagacggtcg ggtccagata ttcgtatctg tcgagtagag tgtgggctca gatgtcgagt
      181 agagtgtggg ctcccacata ctctgatgat ccagacggtc gggtccatct gagacggtcg
      241 ggtccagata ttcgtatctg tcgagtagag tgtgggctca gatgtcgagt agagtgtggg
      301 ctggatcatt catggcaacg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      361 ccaccgctga gcaata
//
