LOCUS       sequence-33462-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral shRNA vector for knockdown of both mouse MKL1 and MKL2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Lee SM, Vasishtha M, Prywes R
  TITLE     Activation and repression of cellular immediate early genes by serum
            response factor cofactors.
  JOURNAL   J Biol Chem. 2010 Jul 16. 285(29):22036-49.
  PUBMED    20466732
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Jul 16. 285(29):22036-49."
COMMENT     Sequence Label: pLKO.1-MKL1/2 shRNA
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..144
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    299..416
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
       61 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      121 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      181 cttgtggaaa ggacgaaaca ccggcatgga gctggtggag aagaactcga gttcttctcc
      241 accagctcca tgtttttgaa ttctcgacct cgagacaaat ggcagtattc atccacaatt
      301 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
      361 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
      421 tttattacag ggacagcaga gatccacttt ggccgcggct cgagggggtt ggggttgcgc
      481 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
      541 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
      601 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
      661 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
      721 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
      781 gct
//
