LOCUS       sequence-334630-       12412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The lentiviral vector for AKAR3EV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12412)
  AUTHORS   Komatsu N, Aoki K, Yamada M, Yukinaga H, Fujita Y, Kamioka Y, 
            Matsuda M
  TITLE     Development of an optimized backbone of FRET biosensors for kinases 
            and GTPases.
  JOURNAL   Mol Biol Cell. 2011 Dec;22(23):4647-56. doi: 
            10.1091/mbc.E11-01-0072. Epub 2011 Oct 5.
  PUBMED    21976697
REFERENCE   2  (bases 1 to 12412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E11-01-0072"; journalName: "Mol Biol Cell"; date: 
            "2011-12"; volume: "22"; issue: "23"; pages: "4647-56"
COMMENT     Sequence Label: pCSIIbsr-AKAR3EV
FEATURES             Location/Qualifiers
     source          1..12412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1676..1909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2094..2138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2412..2529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2585..3747
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2816..3738
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3695..3715
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3782..4492
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELY"
     CDS             4928..5275
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGXGSAGGSAGGSXSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             join(5315..5317,5318..5320,5321..5833)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISGNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(5360..5381)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5621..5640)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             6038..6064
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     misc_feature    join(6078..6652,6653..6655,6656..6664)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6245..6262)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6472..6491
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6665..7063
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7107..7695
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7160..7180)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7578..7589)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8214..8394
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8420..8437)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8426..8650
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8696..9124
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8783..8802)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8993..9014
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9133..9153)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9138..9467
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9318..9453
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9380..9399
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9515..9562
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9581..9955
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10085..10206
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10122..10141)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10176..10195
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10255..10271)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10255..10271)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10268..10290)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10279..10295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10303..10309,10310..10327,10328..10333))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10348..10369
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10486..10503)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10657..11245)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10737..10756)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11416..12207,12208..12276))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12039..12058
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12277..12381)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gggccgctga tcttcagacc tggaggagga gatatgaggg
     1561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2281 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2341 gtggagagag agacagagac agatccattc gattagtgaa cggatctaca aatggcagta
     2401 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2461 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2521 caaaattttc gggtttatta cagggacagc agaaattcac tttgaattaa ttcaagcttc
     2581 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     2641 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     2701 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     2761 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     2821 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2881 aattacttcc acctggctcc agtacgtgat tcttgatccc gagctggagc caggggcggg
     2941 ccttgcgctt taggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     3001 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     3061 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     3121 gtaaatgcgg gccaggatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     3181 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     3241 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     3301 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     3361 gaaagatggc cgcttcccgg ccctgctcca gggggctcaa aatggaggac gcggcgctcg
     3421 ggagagcggg cgggtgagtc acccacacaa aggaaagggg cctttccgtc ctcagccgtc
     3481 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctggagc
     3541 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     3601 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     3661 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     3721 ttttttcttc catttcaggt gtcgtgaaca cgctaccggt ctcgacgaat tcgcccttag
     3781 catgtctaaa ggtgaagaat tattcactgg tgttgtccca attttggttg aattagatgg
     3841 tgatgttaat ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg ctacgtacgg
     3901 taaattgacc ttaaaattac tctgtactac tggtaaattg ccagttccat ggccaacctt
     3961 agtcactact ttaggttatg gtgttcaatg ttttgctaga tacccagatc atatgaaaca
     4021 acatgacttt ttcaagtctg ccatgccaga aggttatgtt caagaaagaa ctattttttt
     4081 caaagatgac ggtaactaca agaccagagc tgaagtcaag tttgaaggtg ataccttagt
     4141 taatagaatc gaattaaaag gtattgattt taaagaagat ggtaacattt taggtcacaa
     4201 attggaatac aactataact ctcacaatgt ttacatcact gctgacaaac aaaagaatgg
     4261 tatcaaagct aacttcaaaa ttagacacaa cattgaagat ggtggtgttc aattagctga
     4321 ccattatcaa caaaatactc caattggtga tggtccagtc ttgttaccag acaaccatta
     4381 cttatcctat caatctgcct tattcaaaga tccaaacgaa aagagagacc acatggtctt
     4441 gttagaattt ttgactgctg ctggtattac cgagggtatg aatgaattgt acctcgagaa
     4501 gttttctcaa gaacagatcg gcgaaaacat tgtgtgcagg gtcatttgta ccacgggtca
     4561 aattcccatc cgagatttgt cagctgatat ttcacaagtg cttaaggaaa aacgatccat
     4621 aaagaaagtt tggacatttg gtagaaaccc agcctgtgac tatcatttag gaaacatttc
     4681 aagactgtca aataagcatt tccaaatact actaggagaa gacggtaacc ttttattgaa
     4741 tgacatttcc actaatggga cctggttaaa tgggcaaaaa gtcgagaaga acagcaatca
     4801 gttactgtcc caaggtgatg aaataaccgt tggtgtaggc gtggaatcag atattttatc
     4861 tctggtcatt ttcataaacg acaaatttaa gcagtgcctg gagcagaaca aagttgatcg
     4921 cggtaccagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc
     4981 tggtggttcc ggcagtgctg gtggtagtgc tggtggtagt accagtgctg gtggtagtgc
     5041 tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggttsyggca gtgctggtgg
     5101 tagtgctggt ggtagtrcca gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc
     5161 tggtggtagt gctggtggtt ccggcagtgc tggtggtagt gctggtggta gtaccagtgc
     5221 tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggttccgg
     5281 attgaggcgc gcgacgctgg ttgacggcgg ccgcatggtg agcaagggcg aggagctgtt
     5341 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     5401 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     5461 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgt cctggggcgt
     5521 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     5581 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     5641 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     5701 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcgg
     5761 gaacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
     5821 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
     5881 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccttaag
     5941 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcttga ccgccgccgg
     6001 gatcactctc ggcatggacg agctgtctag actgcagctg cctcctctgg aacgcctgac
     6061 tctggattaa ggatccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc
     6121 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     6181 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     6241 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     6301 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     6361 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     6421 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     6481 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     6541 gatctggggc ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     6601 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac
     6661 aaccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
     6721 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
     6781 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
     6841 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
     6901 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
     6961 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
     7021 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagtcgagc ctctagagtt
     7081 aacatcgagg gatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     7141 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     7201 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     7261 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     7321 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     7381 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     7441 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     7501 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7561 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7621 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7681 gggccgcctc cccgcatcga taccgtcgag acctagaaaa acatggagca atcacaagta
     7741 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     7801 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     7861 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     7921 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     7981 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     8041 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     8101 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     8161 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     8221 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8281 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8341 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     8401 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     8461 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     8521 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     8581 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     8641 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     8701 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     8761 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     8821 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     8881 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     8941 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     9001 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     9061 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     9121 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     9181 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     9241 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     9301 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     9361 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     9421 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     9481 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     9541 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     9601 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     9661 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     9721 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     9781 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     9841 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     9901 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     9961 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
    10021 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
    10081 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    10141 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    10201 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
    10261 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    10321 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    10381 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    10441 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    10501 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    10561 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    10621 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    10681 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    10741 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    10801 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    10861 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10921 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10981 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    11041 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    11101 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    11161 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    11221 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    11281 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    11341 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    11401 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    11461 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    11521 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    11581 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    11641 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    11701 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    11761 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    11821 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    11881 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    11941 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    12001 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    12061 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    12121 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    12181 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    12241 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    12301 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    12361 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
