LOCUS       sequence-33465-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of human Elk-1 with an HA tag and a 
            mutation in the MAPK phosphorylation site of the transcriptional 
            activation domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Lee SM, Vasishtha M, Prywes R
  TITLE     Activation and repression of cellular immediate early genes by serum
            response factor cofactors.
  JOURNAL   J Biol Chem. 2010 Jul 16. 285(29):22036-49.
  PUBMED    20466732
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Jul 16. 285(29):22036-49."
COMMENT     Sequence Label: pCGN-ELK-1 S383A
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           87..126
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             156..182
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             162..188
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
       61 caccgggacc gatccagcct ccgggggatc ttggtggcgt gaaactcccg cacctcttcg
      121 gccagcgcct tgtagaagcg cgtatggctt ctagctatcc ttatgacgtg cctgactatg
      181 ccagcctggg aggaccttct agaatggacc catctgtgac gctgtggcag tttctgctgc
      241 agctgctgag agagcaaggc aatggccaca tcatctcctg gacttcacgg gatggtggtg
      301 aattcaagct ggtggatgca gaggaggtgg cccggctgtg gggactacgc aagaacaaga
      361 ccaacatgaa ttacgacaag ctcagccggg ccttgcggta ctactatgac aagaacatca
      421 tccgcaaggt gagcggccag aagttcgtct acaagtttgt gtcctaccct gaggtcgcag
      481 ggtgctccac tgaggactgc ccgccccagc cagaggtgtc tgttacctcc accatgccaa
      541 atgtggcccc tgctgctata catgccgccc caggggacac tgtctctgga aagccaggca
      601 cacccaaggg tgcaggaatg gcaggcccag gcggtttggc acgcagcagc cggaacgagt
      661 acatgcgctc gggcctctat tccaccttca ccatccagtc tctgcagccg cagccacccc
      721 ctcatcctcg gcctgctgtg gtgctcccca atgcagctcc tgcaggggca gcagcgcccc
      781 cctcggggag caggagcacc agtccaagcc ccttggaggc ctgtctggag gctgaaga
//
