LOCUS       sequence-334664-       12738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a 
            Ubi-Gal80 marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Allen SE, Koreman GT, Sarkar A, Wang B, Wolfner MF, Han C
  TITLE     Versatile CRISPR/Cas9-mediated mosaic analysis by gRNA-induced 
            crossing-over for unmodified genomes.
  JOURNAL   PLoS Biol. 2021 Jan 14;19(1):e3001061. doi: 
            10.1371/journal.pbio.3001061. eCollection 2021 Jan.
  PUBMED    33444322
REFERENCE   2  (bases 1 to 12738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2021-01-14"; volume: "19(1):e3001061. doi"; pages: " 
            10.1371/journal.pbio.3001061. eCollection 2021 Jan"
COMMENT     Sequence Label: pAC-U63-tgRNA-Gal80
FEATURES             Location/Qualifiers
     source          1..12738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(4986..5004)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5456..5527,5929..6202,6277..6931,6993..7308,7512..7643,
                     7714..8328)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9107..9661,9662..9692)
                     /label=P element 5' end
     rep_origin      complement(9927..10515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10007..10026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10686..11477,11478..11546))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11309..11328
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11547..11651)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11719..11737
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11775..11797)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11897..11916
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    12126..12195
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
      241 gctgcagtcg acgctagcat atgtttgctc acctgtgatt gctcctactc aaatacaaaa
      301 acatcaaatt ttctgtcaat aaagcatatt tatttatatt tattttacag gaaagaattc
      361 cttttaaagt gtattttaac ctataatgaa aaacgattaa aaaaaataca taaaataatt
      421 cgaaaatttt tgaatagccc aggttgataa aaattcattt catacgtttt ataacttatg
      481 cccctaagta ttttttgacc atagtgtttc aattctacat taattttaca gagtagaatg
      541 aaacgccacc tactcagcca agaggcgaaa aggttagctc gccaagcaga gagggcgcca
      601 gtgctcacta ctttttataa ttctcaactt ctttttccag actcagttcg tatatataga
      661 cctattttca atttaacgtc ggggctttga gtgtgtgtag acatcaagca tcggtggttc
      721 agtggtagaa tgctcgcctg ccacgcgggc ggcccgggtt cgattcccgg ccgatgcaca
      781 aggaagagcg aggctcttct tgtgtttaag agctatgctg gaaacagcat agcaagttta
      841 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttgcct
      901 acctggagcc tgagagttgt tcaataaaat aaaaatgttt cgtttttttg ctttcgccag
      961 tatttattat ttttcatcaa tatgtattca atttggtatg tatttagtaa ttgtaatata
     1021 tagacaatgg ttttccgttg acgtacatac atctgacgtg tgtttattta gacataatag
     1081 ttatgttttc acatcttttt aatgttcgct taatgcgtat gcattctagt ggccatggct
     1141 tgctgttctt cgcgttcaaa atctccagct ccattttgct ttcggtgcgc ttgcaatcag
     1201 tactgtccaa aatcgaaaat cgccgaaccg tagtgtgacc gtgcggggct ctgcgaaaat
     1261 aaactttttt aggtatatgg ccacacacgg ggaaagcaca gtggattata tgtattaata
     1321 tattatgcag gttttcatta cttatccaga tgtaagccca cttaaagcga tttaacaatt
     1381 atttgccgaa agagtataaa caaatttcac ataaaaatgg attaagaaaa gcttgtgtaa
     1441 gattatgcgc agcgttgcca gatagctcca tttaaaacac ttcaaaaaca ataagtttag
     1501 aaaatatata cataaatagc agtcgttgcc gcaacgctca acacatcaca cttttaaaac
     1561 accctttacc tacacagaat tactttttaa atttccagtc aagctgcgag tttcaaaatt
     1621 atagccggta gagaagacag tgctatttca aaagcaaact aacaagggtc ttaaatttca
     1681 aaacaccaat cctaacaagc cttggacttt tgtaagttta gatcaaaggt ggcattgcat
     1741 tcaatgtcat ggtaagaagt aggtcgtcta ggtagaaatc ctcattcagc cggtcaagtc
     1801 agtacgagaa aggtctcaat ttgaaattgt cttaaaaata ttttattgtt ttgtactgtg
     1861 gtgagtttaa acgaaaaaca caaaaaaaaa gtgatacaca gaaatcataa aaaattttaa
     1921 tacaaggtat tcgtacgtat caaaaacatt tcggcacaat tttttttctc tgtactaaag
     1981 tgttacgaac actacggtat tttttagtga ttttcaacgg acaccgaagg tatataaaca
     2041 gcgttcgcga acggtcgcct tcaaaaccaa ttgacatttg cagcagcaag tacaagtaga
     2101 aagtaaagcg caatcagcga aaaatttata cttaattgtt ggtgattaaa gtacaattaa
     2161 aagaacattc tcgaaagtca caagaaacgt aagtttttaa ctcgctgtta ccaattagta
     2221 ataagagcaa caagacgttg agtaatttca agaaaaactg catttcaagg tctttgttcg
     2281 gccatttttt ttttattcaa cgctctacgt aattacaaaa taagaaattg gcagccacgc
     2341 atcttgtttt cccaatgaat tggcatcaaa acgcaaacaa atctataaat aaaacttgcg
     2401 tgttgatttt cgccaagatt tattggcaaa ttgtgaaatt cgcagtgacg catttgaaaa
     2461 ttcgagaaat cacgaacgca ctcgagcatt tgtgtgcatg ttattagtta gttgtttagt
     2521 taattgaagt attttaccaa cgaaatccac ttatttttag ctgaaataga gtaggttgct
     2581 taaacaaagc cacgtctgaa aatttcttat tgcttgtagt tgtgacgtca ccatatacac
     2641 acaaaataat gtgtatgcat gcgtttcagc tgtgtatata tacatgcaca cactcgcatt
     2701 atgaaaacga tgacgagcaa cggaacaggt ttctcaacta cctttgttcc tgtttcttcg
     2761 ctttcctttg ttccaatatt cgtagagggt taataggggt ttctcaacaa agttggcgtc
     2821 gataaataag tttcccattt ttattcccca gccaggaagt tagtttcaat agttttgtaa
     2881 tttcaacgaa actcatttga tttcgtacta attttccaca tctctatttt ctgcccgcag
     2941 aataatccaa ctgaataggg aattgggaac ttaaaaaaaa aaatcaaaat gagcggtacc
     3001 gattacaaca aaaggagtag tgtgagtacg gtgccgaatg ctgctcccat tcgcgtgggc
     3061 ttcgtgggat tgaacgcggc taagggttgg gccattaaaa cgcattatcc agccatactg
     3121 cagctgagct cccagttcca aataacagcg ctttattccc ccaagatcga gacgtccatt
     3181 gcgacaattc agaggctgaa gttgtccaac gccacagctt tcccaacact cgagagcttc
     3241 gcctcgagct ccacgatcga tatgattgtg atcgcaatcc aggtggcttc ccactacgag
     3301 gtggtaatgc ccctgctcga gttttcgaag aacaatccta atctgaagta tttgtttgtg
     3361 gaatgggcac tggcctgctc gctcgatcag gcggagtcga tctataaggc cgctgcagag
     3421 cggggcgtgc aaacaataat cagtctgcag ggacgaaaga gcccgtacat tttgcgggca
     3481 aaagagctta tctcccaggg ctacatcggc gatataaata gcatcgagat tgcaggtaac
     3541 ggaggttggt acggttacga gcggccggtg aaaagcccga aatacattta cgagatcgga
     3601 aacggagttg atctggtgac caccacgttc ggtcacacga tagatatatt gcagtacatg
     3661 accagttcgt acttcagccg tatcaatgcc atggtgttta acaacattcc agaacaggag
     3721 ctcattgatg agaggggcaa tcgcctgggc cagcgggtcc caaagactgt cccagatcat
     3781 ctcttgttcc agggcacgct gctcaacgga aatgttcctg tgtcctgttc ctttaaggga
     3841 ggcaagccta cgaaaaagtt caccaagaat ctggtcatag atatccatgg tacaaagggt
     3901 gatctgaagc tggagggtga cgctggattt gcggaaatct cgaatctcgt tctgtattat
     3961 tcgggcaccc gcgccaatga ttttcccctg gccaatggtc agcaggcacc cttggacccc
     4021 ggctacgacg caggcaagga gattatggag gtgtatcacc tccgaaacta caacgcgatc
     4081 gttggtaata tacaccggct ctaccagagt atcagcgatt tccacttcaa caccaagaaa
     4141 atccccgagc ttccatcgca gttcgtaatg caaggcttcg atttcgaagg ctttcccacc
     4201 ctgatggatg ccttgatctt gcatcgcctg attgaaagtg tctacaagtc gaatatgatg
     4261 ggcagtaccc tgaatgtttc caacatctcc cactactcgc tttaatctag aattaattaa
     4321 ctaagccagt cggcaatcgg acgccttcga aacatgcaac actaatgttt aattcagatt
     4381 tcagcagaga caagctaaaa caccgacgag ttgtaatcat ttctgtgcgc cagcatatat
     4441 ttcttatata caacgtaata cataattatg taattctagc atctccccaa cactcacata
     4501 caaacaaaca aaaaatacaa acacacaaaa cgtatttacc cgcacgcatc cttggcgagg
     4561 ttgagtatga aacaaaaaca aaacttaatt tagagcaaag taattacacg aataaattta
     4621 ataaaaaaaa actataataa aaacgccaat catgttattt ccaaaaaaaa aaaaaaaaaa
     4681 actagacgag atgtttgcgc ttgtgtcgat gaatgtaaag aattgcaact acatagatgg
     4741 taattaattt cagctgtaag aagtacaagt ttttcataat tgtgagaaaa gtgcagtagt
     4801 atcgcttaaa gttcagtttt aatcagggca tttagcagct cgaacccaat gtatttgtaa
     4861 gcccaataaa cgtgtaaatg cctagagcaa aatatcacaa ctgtagcttt tcatccgaaa
     4921 gataataatg aatagactga cctgacgttt ttcaaaatgc gactagtggc ctaattctag
     4981 tatgtatgta agttaataaa acccattttt gcggaaagta gataaaaaaa acattttttt
     5041 tttttactgc actggatatc attgaactta tctgatcagt tttaaattta cttcgatcca
     5101 agggtatttg atgtaccagg ttctttcgat tacctctcac tcaaaatgac attccactca
     5161 aagtcagcgc tgtttgcctc cttctctgtc cacagaaata tcgccgtctc tttcgccgct
     5221 gcgtccgcta tctctttcgc caccgtttgt agcgttacgt agcgtcaatg tccgccttca
     5281 gttgcatttt gtcagcggtt tcgtgacgaa gctccaagcg gtttacgcca tcaattaaac
     5341 acaaagtgct gtgccaaaac tcctctcgct tcttattttt gtttgttttt tgagtgattg
     5401 gggtggtgat tggttttggg tgggtaagca ggggaaagtg tgaaaaatcc cggcaatggg
     5461 ccaagaggat caggagctat taattcgcgg aggcagcaaa cacccatctg ccgagcatct
     5521 gaacaatgtg agtagtacat gtgcatacat cttaagttca cttgatctat aggaactgcg
     5581 attgcaacat caaattgtct gcggcgtgag aactgcgacc cacaaaaatc ccaaaccgca
     5641 attgcacaaa caaatagtga cacgaaacag attattctgg tagctgttct cgctatataa
     5701 gacaattttt gagatcatat catgatcaag acatctaaag gcattcattt tcgactatat
     5761 tcttttttac aaaaaatata acaaccagat attttaagct gatcctagat gcacaaaaaa
     5821 taaataaaag tataaaccta cttcgtagga tacttcgggg tactttttgt tcggggttag
     5881 atgagcataa cgcttgtagt tgatatttga gatcccctat cattgcaggg tgacagcgga
     5941 gcggcttcgc agagctgcat taaccagggc ttcgggcagg ccaaaaacta cggcacgctc
     6001 cggccaccca gtccgccgga ggactccggt tcagggagcg gccaactagc cgagaacctc
     6061 acctatgcct ggcacaatat ggacatcttt ggggcggtca atcagccggg ctccggatgg
     6121 cggcagctgg tcaaccggac acgcggacta ttctgcaacg agcgacacat accggcgccc
     6181 aggaaacatt tgctcaagaa cggtgagttt ctattcgcag tcggctgatc tgtgtgaaat
     6241 cttaataaag ggtccaatta ccaatttgaa actcagtttg cggcgtggcc tatccgggcg
     6301 aacttttggc cgtgatgggc agttccggtg ccggaaagac gaccctgctg aatgcccttg
     6361 cctttcgatc gccgcagggc atccaagtat cgccatccgg gatgcgactg ctcaatggcc
     6421 aacctgtgga cgccaaggag atgcaggcca ggtgcgccta tgtccagcag gatgacctct
     6481 ttatcggctc cctaacggcc agggaacacc tgattttcca agccatggtg cggatgccac
     6541 gacatctgac ctatcggcag cgagtggccc gcgtggatca ggtgatccag gagctttcgc
     6601 tcagcaaatg tcagcacacg atcatcggtg tgcccggcag ggtgaaaggt ctgtccggcg
     6661 gagaaaggaa gcgtctggca ttcgcctccg aggctctaac cgatccgccg cttctgatct
     6721 gcgatgagcc cacctccgga ctggactcct ttaccgccca cagcgtcgtc caggtgctga
     6781 agaagctgtc gcagaagggc aagaccgtca tcctgaccat tcatcagccg tcttccgagc
     6841 tgtttgagct ctttgacaag atccttctga tggccgaggg cagggtagct ttcttgggca
     6901 ctcccagcga agccgtcgac ttcttttcct agtgagttcg atgtgtttat taagggtatc
     6961 tagtattaca taacatctca actcctatcc agcgtgggtg cccagtgtcc taccaactac
     7021 aatccggcgg acttttacgt acaggtgttg gccgttgtgc ccggacggga gatcgagtcc
     7081 cgtgatcgga tcgccaagat atgcgacaat tttgccatta gcaaagtagc ccgggatatg
     7141 gagcagttgt tggccaccaa aaatctggag aagccactgg agcagccgga gaatgggtac
     7201 acctacaagg ccacctggtt catgcagttc cgggcggtcc tgtggcgatc ctggctgtcg
     7261 gtgctcaagg aaccactcct cgtaaaagtg cgacttattc agacaacggt gagtggttcc
     7321 agtggaaaca aatgatataa cgcttacaat tcttggaaac aaattcgcta gattttagat
     7381 agaattgcct gattccacac ccttcttagt ttttttcaat gagatgtata gtttatagtt
     7441 ttgcagaaga taaataaatt tcatttaact cgcgaatatt aatgagatgc gagtaacatt
     7501 ttaatttgca gatggttgcc atcttgattg gcctcatctt tttgggccaa caactcacgc
     7561 aagtgggtgt gatgaatatc aacggagcca tcttcctctt cctgaccaac atgacctttc
     7621 aaaacgtctt tgccacgata aatgtaagtc atgtttagaa tacatttgca tttcaataat
     7681 ttactaactt tctaatgaat cgattcgatt taggtgttca cctcagagct gccagttttt
     7741 atgagggagg cccgaagtcg actttatcgc tgtgacacat actttctggg caaaacgatt
     7801 gccgaattgc cgctttttct cacagtgcca ctggtcttca cggcgattgc ctatccgatg
     7861 atcggactgc gggccggagt gctgcacttc ttcaactgcc tggcgctggt cactctggtg
     7921 gccaatgtgt caacgtcctt cggatatcta atatcctgcg ccagctcctc gacctcgatg
     7981 gcgctgtctg tgggtccgcc ggttatcata ccattcctgc tctttggcgg cttcttcttg
     8041 aactcgggct cggtgccagt atacctcaaa tggttgtcgt acctctcatg gttccgttac
     8101 gccaacgagg gtctgctgat taaccaatgg gcggacgtgg agccgggcga aattagctgc
     8161 acatcgtcga acaccacgtg ccccagttcg ggcaaggtca tcctggagac gcttaacttc
     8221 tccgccgccg atctgccgct ggactacgtg ggtctggcca ttctcatcgt gagcttccgg
     8281 gtgctcgcat atctggctct aagacttcgg gcccgacgca aggagtagcc gacatatatc
     8341 cgaaataact gcttgttttt tttttttacc attattacca tcgtgtttac tgtttattgc
     8401 cccctcaaaa agctaatgta attatatttg tgccaataaa aacaagatat gacctataga
     8461 atacaagtat ttccccttcg aacatcccca caagtagact ttggatttgt cttctaacca
     8521 aaagacttac acacctgcat accttacatc aaaaactcgt ttatcgctac ataaaacacc
     8581 gggatatatt ttttatatac atacttttca aatcgcgcgc cctcttcata attcacctcc
     8641 accacaccac gtttcgtagt tgctctttcg ctgtctccca cccgctctcc gcaacacatt
     8701 caccttttgt tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt cggttcgctc
     8761 aaatggttcc gagtggttca tttcgtctca atagaaatta gtaataaata tttgtatgta
     8821 caatttattt gctccaatat atttgtatat atttccctca cagctatatt tattctaatt
     8881 taatattatg actttttaag gtaatttttt gtgacctgtt cggagtgatt agcgttacaa
     8941 tttgaactga aagtgacatc cagtgtttgt tccttgtgta gatgcatctc aaaaaaatgg
     9001 tgggcataat agtgttgttt atatatatca aaaataacaa ctataataat aagaatacat
     9061 ttaatttaga aaatgcttgg atttcactgg aactagaatt aattcggctg ctgctctaaa
     9121 cgacgcattt cgtactccaa agtacgaatt ttttccctca agctcttatt ttcattaaac
     9181 aatgaacagg acctaacgca cagtcacgtt attgtttaca taaatgattt tttttactat
     9241 tcaaacttac tctgtttgtg tactcccact ggtatagcct tcttttatct tttctggttc
     9301 aggctctatc actttactag gtacggcatc tgcgttgagt cgcctccttt taaatgtctg
     9361 accttttgca ggtgcagcct tccactgcga atcattaaag tgggtatcac aaatttggga
     9421 gttttcacca aggctgcacc caaggctctg ctcccacaat tttctcttaa tagcacactt
     9481 cggcacgtga attaatttta ctccagtcac agctttgcag caaaatttgc aatatttcat
     9541 ttttttttat tccacgtaag ggttaatgtt ttcaaaaaaa aattcgtccg cacacaacct
     9601 ttcctctcaa caagcaaacg tgcactgaat ttaagtgtat acttcggtaa gcttcggcta
     9661 tcgacgggac caccttatgt tatttcatca tgggccagac ccacgtagtc cagcggcaga
     9721 tcggcggcgg agaagttaag cgtctccagg atgaccttgc ccgaactggg gcacgtggtg
     9781 ttcgacgatg tgcagctaat ttcgcccggc tccacgtccg cccattggtt aatcagcaga
     9841 ccctcgttgg cgtaacggaa ccatgagagg tacgacaacc atttgaggta tactggcacc
     9901 gagcccgagt tcaagaagaa ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9961 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    10021 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    10081 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10141 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10201 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10261 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10321 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    10381 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    10441 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10501 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    10561 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    10621 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    10681 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    10741 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    10801 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    10861 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    10921 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    10981 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    11041 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    11101 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    11161 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    11221 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    11281 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    11341 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    11401 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    11461 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    11521 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    11581 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    11641 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    11701 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    11761 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
    11821 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
    11881 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatcgacatg
    11941 cccgccgtga ccgtcgagaa cccgctgacg ctgccccgcg tatccgcacc cgccgacgcc
    12001 gtcgcacgtc ccgtgctcac cgtgaccacc gcgcccagcg gtttcgaggg cgagggcttc
    12061 ccggtgcgcc gcgcgttcgc cgggatcaac taccgccacc tcgacccgtt catcatgatg
    12121 gaccagatgg gtgaggtgga gtacgcgccc ggggagccca agggcacgcc ctggcacccg
    12181 caccgcggct tcgagaccgt gacctacatc gtcgatatgc ggtgtgaaat accgcaccga
    12241 atcgcgcgga actaacgaca gtcgctccaa ggtcgtcgaa caaaaggtga atgtgttgcg
    12301 gagagcgggt gggagacagc gaaagagcaa ctacgaaacg tggtgtggtg gaggtgaatt
    12361 atgaagaggg cgcgcgattt gaaaagtatg tatataaaaa atatatcccg gtgttttatg
    12421 tagcgataaa cgagtttttg atgtaaggta tgcaggtgtg taagtctttt ggttagaaga
    12481 caaatccaaa gtctacttgt ggggatgttc gaaggggaaa tacttgtatt ctataggtca
    12541 tatcttgttt ttattggcac aaatataatt acattagctt tttgaggggg caataaacag
    12601 taaacacgat ggtaataatg gtaaaaaaaa aaaacaagca gttatttcgg atatatgtcg
    12661 gctactcctt gcgtcgggcc cgaagtctta gagccagata tgcgagcacc cggaagctca
    12721 cgatgagaat ggccagac
//
