LOCUS       sequence-33473-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of the cytoplasmic domain of human ATF6 
            with mutations eliminating DNA binding and with an HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Wang Y, Shen J, Arenzana N, Tirasophon W, Kaufman RJ, Prywes R
  TITLE     Activation of ATF6 and an ATF6 DNA binding site by the endoplasmic 
            reticulum stress response.
  JOURNAL   J Biol Chem. 2000 Sep 1. 275(35):27013-20.
  PUBMED    10856300
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Sep 1. 275(35):27013-20."
COMMENT     Sequence Label: pCGN-ATF6 (1-373)m1
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           102..141
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             171..197
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             177..203
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gggcattagc gactgttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
       61 gacctccata gaagacaccg ggaccgatcc agcctccggg ggatcttggt ggcgtgaaac
      121 tcccgcacct cttcggccag cgccttgtag aagcgcgtat ggcttctagc tatccttatg
      181 acgtgcctga ctatgccagc ctgggaggac cttctagaat gggggagccg gctggggttg
      241 ccggcaccat ggagtcacct tttagcccgg gactctttca caggctggat gaagattggg
      301 attctgctct ctttgctgaa ctcggttatt tcacagacac tgatgagctg caattggaag
      361 cagcaaatga gacgtatgaa aacaattttg ataatcttga ttttgatttg gatttgttac
      421 cttgggagtc agacatttgg gacatcaaca accaaatctg tacagttaaa gatattaagg
      481 cagaacccca gccactttct ccagcctcct caagttattc agtctcatct cctcggtcag
      541 tggactctta ttcttcaact cagcatgttc ctgaggagtt ggatttgtct tctagttctc
      601 agatgtctcc cctttcctta tatggtgaaa actctaatag tctctcttca ccggagccac
      661 tgaaggaaga taagcctgtc actggttcta ggaacaagac tgaaaatgga ctgactccaa
      721 agaaaaaaat tcaggtgaat tcaaaacctt caattcagcc caagccttta ttgcttccag
      781 cagcacccaa gactcaaaca aactccagtg ttccagcaaa aaccatcatt attcagacag
      841 taccaacgct tatgccattg gcaaagcagc aaccaattat cagtttacaa cctgcaccca
      901 ctaaaggc
//
