LOCUS       sequence-334740-       12406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12406)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1346
FEATURES             Location/Qualifiers
     source          1..12406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1766..1976
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1788..1984
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1835..1970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2016..2618
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2016..2035)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2512..2532
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2661..2778
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2916..4094
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3147..4085
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4042..4062
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4105..4114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8259..8847
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8312..8332)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8730..8741)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8919..9152
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9230..9351
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9267..9286)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9321..9340
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9391..9526
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9546..9565)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9547..9565)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9575..9592)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9575..9591)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9584..9606)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9733..10188
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9820..9839)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10030..10051
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10214..10318
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10319..10387,10388..11179)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10537..10556)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11350..11938
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11839..11858
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12092..12109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12226..12247
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12262..12267,12268..12285,12286..12292)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12300..12316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12305..12327
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12324..12340
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12324..12340
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12359..12379
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12361..12379
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag
     1741 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta
     1801 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1861 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1921 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1981 caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc
     2041 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     2101 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     2161 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     2221 acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     2281 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     2341 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     2401 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     2461 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     2521 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     2581 gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa
     2641 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2701 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2761 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
     2821 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
     2881 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
     2941 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     3001 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     3061 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     3121 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     3181 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     3241 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     3301 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     3361 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     3421 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     3481 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     3541 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     3601 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     3661 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     3721 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     3781 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     3841 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     3901 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     3961 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     4021 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     4081 ttcaggtgtc gtgaggctag caccgccacc atggaccagt acagccttgg agacgagggt
     4141 gccctcccat cagaaatgca cctcccttca ttttcagaga gccaagggct caactgcagc
     4201 gacaccctca accgggattt gggtcccaac acgcgaggct ttctttatgc tggcctgagt
     4261 ggtctggacc cggaccccag cctcccaacg cctgacatgt ccagcgaggt gctggaggac
     4321 aacttagaca ccttgtccct gtactccggg aaggacagtg attctgtgaa gctgctggag
     4381 gagtacgcag attcggagtc tcaggcatcc ttacaagacc tggggctagg tgtgcttaag
     4441 gctaaagagg ctgacgaagg aggaagggcc acctcgggga gtgccaggaa aggaaagcgg
     4501 cagcacagtt cccctcaaaa cccacttctg gactgcagcc tgtgcgggaa ggtgttcagc
     4561 agcgccagtt ctctgagcaa gcactacctg acacacagcc aggaaaggaa gcacgtctgc
     4621 aaaatctgca gcaaggcctt taagcgccag gaccacctga ccgggcacat gctcacccac
     4681 cagaagacaa agcccttcgt gtgcatcgag cagggctgca gcaagagcta ctgcgactac
     4741 cgctctctgc gccggcacta cgaggtccat cacggcctgt gcatcctgaa ggaagccccc
     4801 ccggaggaag aggcctgcgg ggactccccc cacgcccacg agtcggccgg ccagccgccc
     4861 cccagcagcc tgcggtccct ggtgccccca gaggccaggt cccccggctc cctcctgccc
     4921 caccgggacc tcctgcgccg catcgtgagt agcatcgtcc accagaagac cccttctcct
     4981 ggcccagccc cggcgggggc ttcagacagc gaagggagga acactgcctg tccctgcccc
     5041 gcctcatcgg ggtcctcgtc ctgcacccca gccggccccc acgcggcccc agcagcgctg
     5101 gacaccgagc ttcccgagga gccttgcctc ccacagaaag agccggccac tgacgtgttc
     5161 acagccccta attccagggc cgccgagaac ggcgcccccg acccgccaga gccggagcca
     5221 gataccgcgc tgctccaggc ccggtccacc gcggagtgct ggcccgaagg cggctccgtg
     5281 cctgcctgcc tgcctctctt ccgaggccag acggtccctg ccagttccca gccatcgagc
     5341 cacagcttcc agtggctccg gaacctgccg ggctgtccca aaagcaaagg caacaacgtg
     5401 tttgttgtcc acaagccctc ggccgtgccc tcgcgggagg gctccgagtc tggcccggga
     5461 cccagcagcg gaagcccctc ggaggagtcc ccgcccggcc ccggcggtgg cctggaggat
     5521 gctctgccct tccctgccgc gctcctcagg gtccccgcgg aggccccgag cgaccccagg
     5581 tcggccagcg gggaagatga cccctgcgcc cccaagaagg tcaaggtcga ctgcgactcc
     5641 ttcctgtgcc agaaccccgg ggagcccggc ctccaggagg cccagaaggc aggcgggctc
     5701 cctgcggatg cctcgccgct cttccgccag ctcttcctca agtcgcagga acctcttgtg
     5761 agccacgagc agatgcaggt gttccagatg atcaccaagt cccagcggat cttctcccat
     5821 gcccaggtgg cagcagtctc ctcccagctc cctgcgcccg agggcaaacc agccgccctg
     5881 aggccgctgc aggggccgtg gccgcagcag cccccaccac tggctcctgc tgtggactct
     5941 ctccacgccg gccctggaaa ccccgaggca gagggctccc cagcccgcag gagaaaaacc
     6001 acacccgggg ttcccagaga ggcctccccc ggcagcacga gacgagacgc aaagggggga
     6061 ctgaaagtgg ccgcggttcc aacgcccctt gcagcaccgt ctctggaccc ttccaggaat
     6121 ccagacatct cttctctggc caagcagctg cgatcctcta aagggacctt ggacctggag
     6181 gacatcttcc cctccacagg ccaacggcag acccagttag gtggggagga gccaccagga
     6241 gcctcgctgc cagggaagca ggccccagcc gagaatggcg cggcttcaag gatcacaaaa
     6301 ggtgaaaagg gcccagcctg ctcccggggt ggaggctacc ggctcttggg caaccccagg
     6361 gccccgcgat tctccggctt ccggaaagag aaggcgaaga tggatatgtg ctgtgcggct
     6421 tctccgagcc aggtagccat ggcctccttc tcatcggccg ggcccccggc agatccctcc
     6481 aagtccaagc tgacaatatt cagcagaatc cagggtggaa acatctacag gctcccccat
     6541 ccggtgaagg aagagaatgt ggcaggcaga ggtaaccagc aaaacggcag tcccacagac
     6601 tggacgaagc ccaggagcac ttttgtctgc aagaactgca gccagatgtt ttatacggag
     6661 aaagggctga gcagccacat gtgttttcac agcgaccagt ggccgtcacc tcgagggaag
     6721 caggaaccgc aggtgtttgg cacagagttt tgcaagccgc taagacaggt gctgaggcca
     6781 gaaggggaca ggcatagtcc cccaggaacc aagaagccct tggaccccac agctgcagcc
     6841 cctttggtgg tcccccaatc gatccccgtg gttccagtga cccgacacat agggagcatg
     6901 gccatgggac aagagaaaga cggggaggag cgagacagca aggagagcag ccagcagaga
     6961 aagcggaaga agcggccccc accctccacg gctggggagc ctggccctgc aggatgccac
     7021 cagagccgcc tgcggtcacc catgttcctg gtggactgcc tcctgaaggg cttattccag
     7081 tgctccccct acacaccacc cccaatgctc agccccatcc gggagggctc tggggtgtac
     7141 ttcaacaccc tctgttccac gtccactcag gccagccctg accagctcat cagctccatg
     7201 ctcgatcaag tggatgggtc ctttggcatc tgtgtggtga aggatgacac caaaatcagc
     7261 attgagccac atatcaatat aggaagccgg tttcaggcag agatcccgga actccaggag
     7321 agatccctgg ctggaactga cgagcatgta gcttctctgg tctggaagcc atggggagat
     7381 atgatgatca gctcagagac acaggataga gtgaccgagc tctgcaatgt ggcatgctcc
     7441 agcgtgatgc caggaggggg caccaacctg gagctcgctc tgcactgcct gcacgaggct
     7501 cagggcaacg ttcaggtcgc cctggagact cttctgctcc gagggcccca caagccacgg
     7561 acacacctgc tcgctgacta tcgctacaca ggttcagacg tctggacccc tatagagaag
     7621 aggcttttta agaaggcgtt ctatgcccac aagaaggact tctacttgat acacaagatg
     7681 atccagacaa agacggtagc tcagtgcgtt gagtattatt acatctggaa aaaaatgatc
     7741 aagtttgact gtggccgagc cccagggcta gaaaagaggg tcaagagaga gccggaggaa
     7801 gtggaaagga cagaggaaaa ggtcccatgc agccctcggg agagacccag ccaccatcca
     7861 actcccaagt taaagaccaa gagttatagg agggagtcca tcctcagctc cagcccaaat
     7921 gcaggctcca agcggacccc cgagctgttg ggaagtgcag agagccaggg catttttcca
     7981 tgcagagagt gtgaaagggt gtttgacaag atcaagagtc gaaatgccca tatgaagcgg
     8041 caccgccttc aggaccacgt ggagcccatc atcagggtga agtggccagt gaagcccttc
     8101 cagctaaagg aagaggagct gggagctgac atcggccccc tgcagtggta gtaatgaact
     8161 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     8221 gcttaatatg ccgagcctcg tatacgcgtt aagtcgacaa tcaacctctg gattacaaaa
     8281 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     8341 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     8401 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     8461 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     8521 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     8581 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     8641 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     8701 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     8761 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     8821 ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc
     8881 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     8941 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     9001 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9061 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     9121 acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat
     9181 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     9241 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9301 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9361 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     9421 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     9481 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     9541 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     9601 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     9661 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     9721 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     9781 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     9841 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     9901 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     9961 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
    10021 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
    10081 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
    10141 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
    10201 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
    10261 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    10321 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    10381 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    10441 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    10501 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    10561 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    10621 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    10681 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    10741 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    10801 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    10861 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    10921 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    10981 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    11041 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    11101 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    11161 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    11221 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    11281 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    11341 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    11401 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    11461 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    11521 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    11581 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    11641 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    11701 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    11761 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    11821 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    11881 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    11941 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    12001 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    12061 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    12121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    12181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    12241 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
    12301 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
    12361 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaa
//
