LOCUS       sequence-334864-        9115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a kappa-opioid receptor-based circular permuted green 
            fluorescent protein sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9115)
  AUTHORS   Kroning KE, Wang W
  TITLE     Designing a Single Protein-Chain Reporter for Opioid Detection at 
            Cellular Resolution.
  JOURNAL   Angew Chem Int Ed Engl. 2021 Mar 4. doi: 10.1002/anie.202101262.
  PUBMED    33662184
REFERENCE   2  (bases 1 to 9115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2021 Mar 4. doi: 10.1002/anie.202101262."
COMMENT     Sequence Label: K-SPOTIT1.0
FEATURES             Location/Qualifiers
     source          1..9115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..25
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9..25
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     44..64
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        46..64
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        92..318
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             319..499
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    546..671
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1164..1397
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1582..1626
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1934..2051
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2168..2471
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2472..2675
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2626..2646
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4137..4158
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4264..4285)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4525..4544)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    5059..5647
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5112..5132)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5530..5541)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5719..5952
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6030..6151
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6067..6086)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6121..6140
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6191..6326
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6346..6365)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6347..6365)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6375..6392)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6375..6391)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6384..6406)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6533..6988
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6620..6639)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6830..6851
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7014..7118
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7119..7187,7188..7979)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7337..7356)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8150..8738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8639..8658
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8892..8909
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9026..9047
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9062..9067,9068..9085,9086..9092)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    join(9100..9115,1)
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(9105..9115,1..12)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
       61 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
      121 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
      181 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
      241 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
      301 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
      361 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
      421 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
      481 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
      541 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
      601 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
      661 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
      721 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
      781 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
      841 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
      901 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
      961 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     1021 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     1081 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     1141 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     1201 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     1261 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     1321 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     1381 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     1441 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     1501 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     1561 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     1621 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     1681 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     1741 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gagagcgctg
     1801 ccgtctggac cgatggctgt gtggaagtgc tcgccgatag tggaaacaga cgccccagca
     1861 ctcgtcctag ggcaaaggat ctgcagtaat gagaattcta gatcttgaga caaatggcag
     1921 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     1981 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2041 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
     2101 gggcccgggg ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     2161 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     2221 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     2281 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     2341 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     2401 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     2461 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     2521 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     2581 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     2641 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactgtc gggatccacc
     2701 gctagcatga agacgatcat cgccctgagc tacatcttct gcctggtgtt cgccgactac
     2761 aaggacgatg atgacgccat ggacagcccc attcaaatct tccggggcga gcccggacca
     2821 acctgcgctc caagcgcttg cctgccaccg aattctagtg catggttccc agggtgggca
     2881 gagcccgata gcaatggctc agccgggtcc gaggatgcgc agcttgaacc cgctcacatc
     2941 tcacccgcta tccctgtcat tattacggca gtctactcag ttgttttcgt tgtgggcctg
     3001 gttggaaata gcctcgtgat gtttgtgatt atcaggtata ctaaaatgaa gaccgccacg
     3061 aatatctaca tctttaacct ggctctggct gacgccttgg tgaccaccac tatgccattt
     3121 cagtccaccg tctatcttat gaactcttgg ccattcggcg acgttctgtg caagattgtc
     3181 atttccattg actattataa tatgtttaca tcaatcttta cgcttacaat gatgagcgtg
     3241 gaccgataca ttgctgtttg ccatccggtg aaagcactgg attttcgcac ccccttgaag
     3301 gccaagatta tcaatatctg tatttggctc ctgtcctcat ccgtcggaat ttccgccatt
     3361 gtcttgggtg gtacaaaggt gagggaggac gtggacgtta tagagtgcag cctgcagttc
     3421 cctgacgatg actacagctg gtgggacctt tttatgaaga tctgtgtctt catttttgct
     3481 ttcgtcatcc ccgtgctgat tatcatagtg tgctatacac tgatgatatt gcggctgaaa
     3541 tctgtgcgcc tgctgagcgg cagccgcgaa aaggatcgga atctgcgccg aatcacacgc
     3601 ctcgtgctgg ttgtagtcgc tgtcttcgtg gtatgctgga ccccaatcca cattttcatc
     3661 ctggtggaag ctctgggatc cacctctcac tccactgccg cacttagctc atattacttc
     3721 tgcattgcct tgggctacac taattcctca ctgaatccca ttctgtacgc atttcttgac
     3781 gagaacttca aacggtgctt tagagacttt tgcttccccc tcaagatgcg catggagcgg
     3841 cagtcaacca gccgagtccg gaatacggtc caggatcccg cctatctgcg ggacatcgac
     3901 ggcatgaaca agcctgtgca gggcgcgccc aacgtctata tcaaggccga caagcagaag
     3961 aacggcatca aggcgaactt ccacatccgc cacaacatcg aggacggcgg cgtgcagctc
     4021 gcctaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     4081 cactacctga gcgtgcagtc caaactttcg aaagacccca acgagaagcg cgatcacatg
     4141 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     4201 ggcggtaccg gagggagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     4261 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt
     4321 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     4381 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     4441 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacatc
     4501 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     4561 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     4621 ggcaacatcc tggggcacaa gctggagtac aaccttaagg aagatatcat ggctcaagtc
     4681 caacttgtag agtctggagg aggtctcgtg cggcccggcg gtagcctgcg actctcctgt
     4741 gttgattcag agaggacgag ctaccctatg gggtggttta gacgggcacc cggaaaggag
     4801 cgcgaattcg ttgcaagtat cacgtggagt ggcattgacc cgacttatgc tgatagcgtt
     4861 gccgaccggt ttactacgtc cagagacgtt gcgaataaca cgctgtacct ccagatgaac
     4921 tctctcaagc acgaagatac agcggtctat tactgtgctg caagagcacc cgtcggtcaa
     4981 tcttcctctc cttatgatta tgactattgg ggacagggga ctcaggtaac tgtgtccagt
     5041 tagacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     5101 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     5161 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     5221 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     5281 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     5341 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5401 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     5461 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     5521 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5581 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5641 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     5701 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     5761 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5821 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     5881 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     5941 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     6001 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     6061 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6121 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     6181 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6241 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6301 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     6361 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     6421 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6481 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     6541 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6601 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6661 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6721 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6781 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6841 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6901 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6961 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     7021 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7081 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7141 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7201 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7261 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7321 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7381 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7441 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7501 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7561 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7621 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7681 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7741 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7801 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7861 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7921 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7981 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8041 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8281 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8761 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8821 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8881 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8941 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     9001 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     9061 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taaca
//
