LOCUS       sequence-334914-        8736 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generation of SARS-CoV-2 spike pseudotyped lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Filbin MR, Mehta A, Schneider AM, Kays KR, Guess JR, Gentili M, 
            Fenyves BG, Charland NC, Gonye ALK, Gushterova I, Khanna HK, LaSalle
            TJ, Lavin-Parsons KM, Lilley BM, Lodenstein CL, Manakongtreecheep K,
            Margolin JD, McKaig BN, Rojas-Lopez M, Russo BC, Sharma N, Tantivit 
            J, Thomas MF, Gerszten RE, Heimberg GS, Hoover PJ, Lieb DJ, Lin B, 
            Ngo D, Pelka K, Reyes M, Smillie CS, Waghray A, Wood TE, Zajac AS, 
            Jennings LL, Grundberg I, Bhattacharyya RP, Parry BA, Villani AC, 
            Sade-Feldman M, Hacohen N, Goldberg MB
  TITLE     Longitudinal proteomic analysis of severe COVID-19 reveals 
            survival-associated signatures, tissue-specific cell death, and 
            cell-cell interactions.
  JOURNAL   Cell Rep Med. 2021 May 18;2(5):100287. doi: 
            10.1016/j.xcrm.2021.100287. Epub 2021 May 3.
  PUBMED    33969320
REFERENCE   2  (bases 1 to 8736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Med."; date: "2021-05-18"; volume: "2(5):100287. doi"; pages: " 
            10.1016/j.xcrm.2021.100287. Epub 2021 May 3"
COMMENT     Sequence Label: pCMV-SARS2S-Delta-C-H2gp41
FEATURES             Location/Qualifiers
     source          1..8736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(35..52)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(206..794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(286..305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(965..1756,1757..1825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1588..1607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1826..1930)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1956..2411)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2093..2114)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2305..2324
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2538..2560
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2552..2569
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2553..2569
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2579..2598
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2579..2597
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        2718..3097
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3098..3301
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3252..3272
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3298..3322
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          3413..3985
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(8062..8081)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8127..8182
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8181..8200)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8540..8556)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8540..8556)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8553..8575)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8564..8580
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8588..8594,8595..8612,8613..8618))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8633..8654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
       61 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1921 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     1981 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     2041 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     2101 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     2161 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     2221 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     2281 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     2341 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     2401 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2461 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2521 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
     2581 atacgactca ctatagggcg aattggagct ccaccgcggt ggcggccgct ctagagagct
     2641 tggcccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc
     2701 aacattaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
     2761 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2821 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     2881 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     2941 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     3001 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     3061 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     3121 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     3181 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     3241 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     3301 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     3361 aagacaccgg gaccgatcca gcctccggtc gaccgatcct gagaacttca gggtgagttt
     3421 ggggaccctt gattgttctt tctttttcgc tattgtaaaa ttcatgttat atggaggggg
     3481 caaagttttc agggtgttgt ttagaatggg aagatgtccc ttgtatcacc atggaccctc
     3541 atgataattt tgtttctttc actttctact ctgttgacaa ccattgtctc ctcttatttt
     3601 cttttcattt tctgtaactt tttcgttaaa ctttagcttg catttgtaac gaatttttaa
     3661 attcactttt gtttatttgt cagattgtaa gtactttctc taatcacttt tttttcaagg
     3721 caatcagggt atattatatt gtacttcagc acagttttag agaacaattg ttataattaa
     3781 atgataaggt agaatatttc tgcatataaa ttctggctgg cgtggaaata ttcttattgg
     3841 tagaaacaac tacaccctgg tcatcatcct gcctttctct ttatggttac aatgatatac
     3901 actgtttgag atgaggataa aatactctga gtccaaaccg ggcccctctg ctaaccatgt
     3961 tcatgccttc ttctctttcc tacagctcct gggcaacgtg ctggttgttg tgctgtctca
     4021 tcattttggc aaagaattcc tcgacggatc cctcgaggaa ttctgacact atgttcgtgt
     4081 tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc agaacacagc
     4141 tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac aaggtgttca
     4201 gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc aacgtgacct
     4261 ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac aaccccgtgc
     4321 tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc atcagaggct
     4381 ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg aacaacgcca
     4441 ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc ctgggcgtct
     4501 actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac agcagcgcca
     4561 acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa ggcaagcagg
     4621 gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac ttcaaaatct
     4681 acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc tctgctctgg
     4741 aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca ctgctggccc
     4801 tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct ggtgccgccg
     4861 cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac gagaacggca
     4921 ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag tgcaccctga
     4981 agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg cagcccaccg
     5041 aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag gtgttcaatg
     5101 ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat tgcgtggccg
     5161 actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac ggcgtgtccc
     5221 ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc gtgatccggg
     5281 gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac tacaactaca
     5341 agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac ctggactcca
     5401 aagtcggcgg caactacaat tacctgtacc ggctgttccg gaagtccaat ctgaagccct
     5461 tcgagcggga catcagcacc gaaatctatc aggccggcag caccccttgc aacggcgtgg
     5521 aaggcttcaa ctgctacttc ccactgcagt cctacggctt tcagcccaca aatggcgtgg
     5581 gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc cctgccacag
     5641 tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac ttcaacttca
     5701 acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg ccattccagc
     5761 agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag acactggaaa
     5821 tcctggacat caccccatgc agcttcggcg gagtgtctgt gatcacccct ggcaccaaca
     5881 ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg taccgaagtg cccgtggcca
     5941 ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc aatgtgtttc
     6001 agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac gagtgcgaca
     6061 tccccatcgg cgctggcatc tgtgccagct accagacaca gacaaacagc cccagacggg
     6121 ctagaagcgt ggccagccag agcatcattg cctacacaat gtctctgggc gccgagaaca
     6181 gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc agcgtgacca
     6241 ccgagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg tacatctgcg
     6301 gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc acccagctga
     6361 atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag gtgttcgccc
     6421 aagtgaagca aatctacaag acccctccta tcaaggactt cggcggcttc aatttcagcc
     6481 agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac ctgctgttca
     6541 acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt ctgggcgaca
     6601 ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg ctgcctcctc
     6661 tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc acaatcacaa
     6721 gcggctggac atttggagct ggcgccgctc tgcagatccc ctttgctatg cagatggcct
     6781 accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag aagctgatcg
     6841 ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc acagcaagcg
     6901 ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac accctggtca
     6961 agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc ctgagcagac
     7021 tggacaaggt ggaagccgag gtgcagatcg acagactgat caccggaagg ctgcagtccc
     7081 tgcagaccta cgttacccag cagctgatca gagccgccga gattagagcc tctgccaatc
     7141 tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg gacttttgcg
     7201 gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg gtgtttctgc
     7261 acgtgacata cgtgcccgct caagagaaga atttcaccac cgctccagcc atctgccacg
     7321 acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc cattggttcg
     7381 tgacccagcg gaacttctac gagccccaga tcatcaccac cgacaacacc ttcgtgtctg
     7441 gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct ctgcagcccg
     7501 agctggacag cttcaaagag gaactggata agtactttaa gaaccacaca agccccgacg
     7561 tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag aaagagatcg
     7621 accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg caagaactgg
     7681 ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt atcgccggac
     7741 tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc tgtagctgcc
     7801 tgaagggcaa cagggtgagg cagggctaca gctgactcaa atcctgcaca acagattctt
     7861 catgtttgga ccaaatcaac ttgtgatacc atgctcaaag aggcctcaat tatatttgag
     7921 tttttaattt ttatgaaaaa aaaaaaaaaa aacggaattc ctcgagggat ccgtcgagga
     7981 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
     8041 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     8101 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     8161 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     8221 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     8281 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     8341 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     8401 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     8461 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacggatc
     8521 ggccgcaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8581 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8641 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8701 gccagctgca ttaatgaatc ggccaacgcg cgggga
//
