LOCUS       sequence-334937-        1415 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused 
            to SUMO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   MacDonald EA, Frey G, Namchuk MN, Harrison SC, Hinshaw SM, Windsor 
            IW
  TITLE     Recognition of Divergent Viral Substrates by the SARS-CoV-2 Main 
            Protease.
  JOURNAL   ACS Infect Dis. 2021 Sep 10;7(9):2591-2595. doi: 
            10.1021/acsinfecdis.1c00237. Epub 2021 Aug 26.
  PUBMED    34437808
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsinfecdis.1c00237"; journalName: "ACS Infect Dis"; date: 
            "2021-09-10- 10"; volume: "7"; issue: "9"; pages: "2591-2595"
COMMENT     Sequence Label: pET-His6-SUMO-MproC145A
FEATURES             Location/Qualifiers
     source          1..1415
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..61
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             81..98
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             117..410
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 aagcggccgc cctgcaggac tcgagttcta gaaataattt tgtttaactt taagaaggag
       61 atatacatat gaaatcttct caccatcacc atcaccatgg ttcttctatg gctagcatgt
      121 cggactcaga agtcaatcaa gaagctaagc cagaggtcaa gccagaagtc aagcctgaga
      181 ctcacatcaa tttaaaggtg tccgatggat cttcagagat cttcttcaag atcaaaaaga
      241 ccactccttt aagaaggctg atggaagcgt tcgctaaaag acagggtaag gaaatggact
      301 ccttaagatt cttgtacgac ggtattagaa ttcaagctga tcagacccct gaagatttgg
      361 acatggagga taacgatatt attgaggctc acagagaaca gattggtggg tcggggtttc
      421 gcaaaatggc gtttccgtcc ggcaaagtgg aaggttgtat ggtacaggtg acatgcggca
      481 ccacaaccct gaatgggttg tggttagacg acgtagtcta ttgccctcgt catgtcatct
      541 gcacctctga ggacatgctc aatccgaatt acgaagatct cctgatccgc aaatccaacc
      601 acaacttcct cgttcaagct ggcaatgttc aactgcgcgt tattggtcat agcatgcaga
      661 attgcgtcct taaactgaaa gttgataccg ccaacccgaa aaccccgaag tacaagtttg
      721 tgcgtattca gcctggtcag accttttcag tgttagcgtg ctataacggc agtccctctg
      781 gtgtgtatca gtgtgctatg cgtccaaact tcacgatcaa aggcagcttc cttaatggca
      841 gcgcgggttc ggtgggcttt aacatcgact acgattgcgt tagcttctgc tatatgcacc
      901 acatggaatt gccgactggt gtacatgccg ggacggactt agaaggcaac ttttatggtc
      961 cgtttgtcga tcgccaaaca gcccaagccg caggaaccga tacgaccatt actgtgaatg
     1021 ttctggcatg gctttatgcg gcggtaatca acggagatcg gtggtttctg aaccgcttta
     1081 ccaccacctt gaacgatttc aacctggtcg cgatgaagta caactacgag ccactgacgc
     1141 aggatcatgt tgacattctg ggaccgttgt cagcacagac gggcattgct gtgctggata
     1201 tgtgtgccag tctgaaagaa ctgctgcaga atgggatgaa tggccgcact attctgggta
     1261 gtgcgttact ggaagatgag ttcactccct tcgatgtggt gcgtcagtgt agcggtgtca
     1321 cgtttcagta atagcggatc cgaattcgag cgccacctgg tggccggccg gtaccacgcg
     1381 tgcgcgctga tccggctgct aacaaagccc gaaag
//
