LOCUS       sequence-335145-        9276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of His-GFP-Rim2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Prasai B, Haber GJ, Strub MP, Ahn R, Ciemniecki JA, Sochacki KA, 
            Taraska JW
  TITLE     The nanoscale molecular morphology of docked exocytic dense-core 
            vesicles in neuroendocrine cells.
  JOURNAL   Nat Commun. 2021 Jun 25;12(1):3970. doi: 10.1038/s41467-021-24167-9.
  PUBMED    34172739
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-25"; volume: "12(1):3970. doi"; pages: " 
            10.1038/s41467-021-24167-9"
COMMENT     Sequence Label: His-GFP-Rim2
FEATURES             Location/Qualifiers
     source          1..9276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             625..642
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(652..654,655..1365)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(652..654,655..1365)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(694..715)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(955..974)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1302..1323
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6064..6185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6101..6120)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6155..6174
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6192..6647)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6329..6350)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6541..6560
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6674..6778
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6780..7137
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6780..6971
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6803..6823)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6988..7123
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7172..7966
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7172..7966
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7226..7245)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7836..7855
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8154..8173)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8198..8245
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8574..9162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9063..9082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatgggcag cagccatcat catcatcatc acagcagcgg cgtgagcaag
      661 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      721 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      781 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      841 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      901 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      961 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1021 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1081 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1141 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1201 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1261 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1321 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct
     1381 cgagctcaag cttcgaattc tatgtcggct ccactcgggc cccggggccg cccggctccc
     1441 accccggcgg cctctcagcc tcctccgcag cccgagatgc cggacctcag ccacctcacg
     1501 gaagaggaga ggaaaatcat ccaggctgtc atggatcgtc agaagaaaga agaggagaag
     1561 gagcagtccg tgctcaaaaa gctgcatcaa caatttgaaa tgtataagga gcaagtcaag
     1621 aaaatgggag aggaatcaca acagcagcaa gagcagaagg gcgacgcccc gacctgcggc
     1681 atctgccaca agacaaaatt tgcagatgga tgcggccata actgttcata ttgccaaacc
     1741 aagttctgtg ctcgttgtgg aggtcgagtg tcattacgct caaacaaggt tatgtgggtg
     1801 tgtaatttgt gccgaaaaca acaagaaatc ctcactaagt cgggcgcgtg gttttacaat
     1861 agtgggtcta atacaccgca gcagcctgat caaaaggctc ttcgagggct tcgcagtgag
     1921 gaagcccctc aggagaagaa ggcaaaactg catgagcaga cgcagttcca gggacccccc
     1981 ggtgactcat cagtacctgc agttgagaga ggtcgagctc atgggctcac aagacaggat
     2041 tctattaaga atggctcagg aatgaagcac cagatcgcca gtgacatgcc ttcagacaga
     2101 aaaagaagtc cgtcagtgtc cagggatcaa aatcgaagat acgaccaaag tgaagaaaga
     2161 gaggaatatt cacagtatgt tccttcagat agcacaatgc ctagatctcc atcagattat
     2221 gctgatagac gatctcaacg tgaacctcaa ttttatgaag aacctgatca tttaaattat
     2281 agggattcta acaggagagg ccatagacat tccaaagagt atattgtaga cgacgaagat
     2341 gtggagagca gagatgaata tgaaagacaa aggagagagg aggagtacca ggcacgctac
     2401 agaagtgatc caaatttggc ccggtatccg gtaaagccac aaccctatga agagcaaatg
     2461 cggatccacg ctgaagtgtc ccgggcacga cacgagagaa ggcacagtga tgtttctttg
     2521 gcaaatgctg aactagaaga ttccaggatt tctctgctaa ggatggatag accatcaagg
     2581 caaagatctg tatctgaacg tagagctgca atggaaaacc agcgatcgta ttcaatggaa
     2641 agaactcgag aagctcaggg acaaagttct tatccacaaa ggaccacaaa tcatagtcct
     2701 cctacccctc gcaggagccc tataccgctc gatagaccag agctgaggcg tgccgactcc
     2761 ctacggaaac aacaccactt agatcccagc tctgctgtaa ggaaaacgaa gcgagaaaaa
     2821 atggaaacaa tgttgaggaa tgattctttg agttcagacc agtctgagtc agtgaggccg
     2881 cccccaccaa ggcctcataa atccaagaaa ggaggtaaaa tgcgccaggt ttcactgagc
     2941 agctcagagg aggagttggc atccacgccc gagtatacaa gctgtgatga cgtggagatt
     3001 gaaagcgaga gcgtgagtga gaaaggagac atggagtaca gctggttgga gcatgcgtct
     3061 tggcatagca gtgaggcatc cccaatgtct ttgcaccctg tgacctggca gccatccaaa
     3121 gatggagatc gcctaattgg tcgtatttta ttaaataagc gtttaaaaga tgggagtgta
     3181 cctcgagatt caggagcaat gctgggctta aaggttgtag gaggaaagat gactgaatca
     3241 ggtcgacttt gtgcatttat taccaaagtg aaaaaaggaa gtttagctga tactgtagga
     3301 catcttagac caggtgatga agtcttggaa tggaatggaa ggctattgca aggagccaca
     3361 tttgaggaag tttacaacat tattctagaa tccaagcctg aaccacaagt tgagcttgtt
     3421 gtttcaaggc cgattggaga tatgcctaga atacctgata gcactcatgc acaactggaa
     3481 tccagttcta gctcatttga atctcaaaaa atggatcgtc cgtctatatc ggttacctct
     3541 cccatgagtc ctggcatgct gagggatgtc ccacagttct tatctggaca gctttcaata
     3601 aaactatggt ttgacaaggt tggtcaccag ttaatagtta caattttggg agcaaaggat
     3661 ctcccttcca gggaagatgg gaggccaagg aatccttatg ttaaaattta cttccttcca
     3721 gacagaagtg ataaaaacaa gagaagaaca aaaacagtca agaaaacttt ggaacccaaa
     3781 tggaaccaga ctttcattta ttctcctgtt caccgaagag aattccggga acgaatgctc
     3841 gaaatcaccc tttgggatca agctcgagtt cgagaggaag aaagtgaatt cctaggagag
     3901 attttaattg aattggaaac agctctgtta gatgatgaac cacactggta caaacttcag
     3961 acccatgatg tctcctcatt gccactccct cacccttctc catatatgcc acggagacag
     4021 ctgcatggag agagcccaac acgcaggttg caaaggtcga agagaataag tgacagcgaa
     4081 gtatccgact atgactgcga ggacggcgtg ggagtcgtgt cagattaccg acacgatggc
     4141 cgtgatcttc aaagctccac attatcagtg ccagaacaag tcatgtcatc aaaccattgc
     4201 tcaccatcag gctctcctca tcgagtagat gttataggaa ggactaggtc atggtcgcct
     4261 agtgtccctc ctcctcaaag gaatgtggaa caggggcttc gagggacacg tgctactggc
     4321 cattacaata caattagccg gatggacaga caccgtgtca tggacgacca ttactcttca
     4381 gagagagaca gggattgtga agcagcagat agacagccat atcacagatc cagatcaaca
     4441 gaacaacggc ctctcctaga gcggaccacc acccgctcca gatcctctga acgtgctgat
     4501 acaaacctca tgaggtcgat gccttcatta atgactggaa gatctgcccc tccttcacct
     4561 gccttatcga ggtctcaccc tcgcactggc tctgtccaga ccagtccgtc aagtactccc
     4621 gtaacaggac gaaggggccg gcagcttcct cagctcccac caaagggaac actggaaaga
     4681 agtgctatgg atatagagga gagaaatcgc caaatgaaac ttaacaaata caaacaggta
     4741 gccggatcag atcccagact ggaacaagat taccattcga agtatcgctc aggatgggat
     4801 ccacatagag gggcagatac tgtttctact aaatcctcgg acagtgatgt aagtgatgta
     4861 tctgcggttt caaggactag tagtgcttct cgtttcagca gcacaagcta catgtccgtc
     4921 caatcagaac ggccgagagg aaacaggaaa atcagtgtct ttacgtccaa aatgcaaagc
     4981 agacagatgg gtgtgtcggg gaagagcatg gccaaaagca ccagcatcag tggagacatg
     5041 tgctcactgg agaagaatga tggcagccag tcggacactg cagtgggcgc cctgggcacc
     5101 agtggcaaga agcggcgatc tagcattggg gccaaaatgg tagctattgt tggtctctca
     5161 cggaaaagtc gcagtgcctc ccaactcagc caaaccgaag gaggaggtaa aaagctacgc
     5221 agcactgtcc agagaagcac ggagaccggg ctagcagtgg agatgaggaa ctggatgaca
     5281 agacaggcca gccgggaatc cacggatggc agcatgaaca gttacagctc agaaggaaat
     5341 ctgatcttcc ctggggtccg cctggcctct gacagccagt tcagcgattt ccttgatggt
     5401 ctgggccctg ctcagctagt gggacgccag accctggcga ctcctgcaat gggtgacatt
     5461 caggtgggga tgatggataa gaagggacag ctggaggtag agatcatccg ggctcgaggc
     5521 cttgtagtaa aaccaggttc caagacactg ccagcaccat atgtcaaggt gtacctgtta
     5581 gacaacggag tctgcatagc caaaaagaaa accaaggtgg cgagaaagac gctggagccc
     5641 ctgtatcagc agctgttatc tttcgaagag agcccccagg ggaaggtgtt acagatcatt
     5701 gtctggggag attatggccg catggatcac aaatccttta tgggagtggc ccagatactc
     5761 ttagatgaac tggaactatc caacatggta attggatggt tcaaactctt ccctccttcc
     5821 tccctagtag atccaacctt ggcacctctg acaagaagag cttcccaatc gtctctggaa
     5881 agttctactg gaccttctta ctctcgttca tgagtcgacg gtaccgcggg cccgggatcc
     5941 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
     6001 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     6061 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6121 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6181 tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     6241 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     6301 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     6361 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     6421 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     6481 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     6541 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     6601 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     6661 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6721 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     6781 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     6841 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     6901 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6961 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     7021 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     7081 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     7141 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     7201 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     7261 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     7321 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     7381 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     7441 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     7501 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     7561 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     7621 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     7681 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     7741 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     7801 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     7861 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     7921 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     7981 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     8041 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     8101 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     8161 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     8221 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     8281 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     8341 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     8401 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     8461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8701 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     8761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     9001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     9061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9241 cctgattctg tggataaccg tattaccgcc atgcat
//
