LOCUS       sequence-335227-        9782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive Expression plasmid with mCherry fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Gebhardt MJ, Jacobson RK, Shuman HA
  TITLE     Seeing red; the development of pON.mCherry, a broad-host range 
            constitutive expression plasmid for Gram-negative bacteria.
  JOURNAL   PLoS One. 2017 Mar 3;12(3):e0173116. doi: 
            10.1371/journal.pone.0173116. eCollection 2017.
  PUBMED    28257493
REFERENCE   2  (bases 1 to 9782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-03-3"; volume: "12(3):e0173116. doi"; pages: " 
            10.1371/journal.pone.0173116. eCollection 2017"
COMMENT     Sequence Label: pON.mCherry
FEATURES             Location/Qualifiers
     source          1..9782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(3..8,9..24,25..31)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     primer_bind     complement(871..888)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1041..1127
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1219..1246
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1265..1356
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1799..1901
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1902..2561
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1968..1987)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2893..3287
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            3629..3716
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             4959..5930
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             6441..7280
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             7267..8118
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(8395..9477)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9439..9458
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9478..9555)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
        1 tgttgacaat taatcatcgg ctcgtataat gtgtggaaag gcggttcacc gccgttttca
       61 cacaggaaac agaattcttt aagaaggaga tatacatatg gtttccaagg gcgaggagga
      121 taacatggct atcattaaag agttcatgcg cttcaaagtt cacatggagg gttctgttaa
      181 cggtcacgag ttcgagatcg aaggcgaagg cgagggccgt ccgtatgaag gcacccagac
      241 cgccaaactg aaagtgacta aaggcggccc gctgcctttt gcgtgggaca tcctgagccc
      301 gcaatttatg tacggttcta aagcgtatgt taaacaccca gcggatatcc cggactatct
      361 gaagctgtct tttccggaag gtttcaagtg ggaacgcgta atgaattttg aagatggtgg
      421 tgtcgtgacc gtcactcagg actcctccct gcaggatggc gagttcatct ataaagttaa
      481 actgcgtggt actaattttc catctgatgg cccggtgatg cagaaaaaga cgatgggttg
      541 ggaggcgtct agcgaacgca tgtatccgga agatggtgcg ctgaaaggcg aaattaaaca
      601 gcgcctgaaa ctgaaagatg gcggccatta tgacgctgaa gtgaaaacca cgtacaaagc
      661 caagaaacct gtgcagctgc ctggcgcgta caatgtgaat attaaactgg acatcacctc
      721 tcataatgaa gattatacga tcgtagagca atatgagcgc gcggagggtc gtcattctac
      781 cggtggcatg gatgagctgt acaaataatc tagagtcgac ctgcaggcat gcaagcttgg
      841 ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag
      901 cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat
      961 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
     1021 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1081 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
     1141 atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
     1201 ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
     1261 ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1321 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     1381 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     1441 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     1501 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgtgatgt ccggcggtgc
     1561 ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga gggacagctg atagaaacag
     1621 aagccactgg cctcaaaaac accatcatac actaaatcag taagttggca gcatcacccg
     1681 acgcactttg cgccgaataa atacctgtga cggaagatca cttcgcagaa taaataaatc
     1741 ctggtgtccc tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg
     1801 atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat
     1861 tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     1921 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     1981 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     2041 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     2101 tgaatgctca tccggaatta cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     2161 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     2221 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     2281 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     2341 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     2401 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     2461 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     2521 aattacaaca gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
     2581 gtcttcaaat tcccgttgca catagcccgg caattccttt ccctgctctg ccataagcgc
     2641 agcgaatgcc gggtaatact cgtcaacgat ctgatagaga agggtttgct cgggtcggtg
     2701 gctctggtaa cgaccagtat cccgatcccg gctggccgtc ctggccgcca catgaggcat
     2761 gttccgcgtc cttgcaatac tgtgtttaca tacagtctat cgcttagcgg aaagttcttt
     2821 taccctcagc cgaaatgcct gccgttgcta gacattgcca gccagtgccc gtcactcccg
     2881 tactaactgt cacgaacccc tgcaataact gtcacgcccc cctgcaataa ctgtcacgaa
     2941 cccctgcaat aactgtcacg cccccaaacc tgcaaaccca gcaggggcgg gggctggcgg
     3001 ggtgttggaa aaatccatcc atgattatct aagaataatc cactaggcgc ggttatcagc
     3061 gcccttgtgg ggcgctgctg cccttgccca atatgcccgg ccagaggccg gatagctggt
     3121 ctattcgctg cgctaggcta cacaccgccc caccgctgcg cggcaggggg aaaggcgggc
     3181 aaagcccgct aaaccccaca ccaaaccccg cagaaatacg ctggagcgct tttagccgct
     3241 ttagcggcct ttccccctac ccgaagggtg ggggcgcgtg tgcagccccg cagggcctgt
     3301 ctcggtcgat cattcagccc ggctcatcct tctggcgtgg cggcagaccg aacaaggcgc
     3361 ggtcgtggtc gcgttcaagg tacgcatcca ttgccgccat gagccgatcc tccggccact
     3421 cgctgctgtt caccttggcc aaaatcatgg cccccaccag caccttgcgc cttgtttcgt
     3481 tcttgcgctc ttgctgctgt tcccttgccc gcacccgctg aatttcggca ttgattcgcg
     3541 ctcgttgttc ttcgagcttg gccagccgat ccgccgcctt gttgctcccc ttaaccatct
     3601 tgacacccca ttgttaatgt gctgtctcgt aggctatcat ggaggcacag cggcggcaat
     3661 cccgacccta ctttgtaggg gagggcgcac ttaccggttt ctcttcgaga aactggccta
     3721 acggccaccc ttcgggcggt gcgctctccg agggccattg catggagccg aaaagcaaaa
     3781 gcaacagcga ggcagcatgg cgatttatca ccttacggcg aaaaccggca gcaggtcggg
     3841 cggccaatcg gccagggcca aggccgacta catccagcgc gaaggcaagt atgcccgcga
     3901 catggatgaa gtcttgcacg ccgaatccgg gcacatgccg gagttcgtcg agcggcccgc
     3961 cgactactgg gatgctgccg acctgtatga acgcgccaat gggcggctgt tcaaggaggt
     4021 cgaatttgcc ctgccggtcg agctgaccct cgaccagcag aaggcgctgg cgtccgagtt
     4081 cgcccagcac ctgaccggtg ccgagcgcct gccgtatacg ctggccatcc atgccggtgg
     4141 cggcgagaac ccgcactgcc acctgatgat ccgtcgatcg acggatcaat gacggcatcg
     4201 agcggcccgc cgctcagtgg ttcaagcggt acaacggcaa gaccccggag aagggcgggg
     4261 cacagaagac cgaagcgctc aagcccaagg catggcttga gcagacccgc gaggcatggg
     4321 ccgaccatgc caaccgggca ttagagcggg ctggccacga cgcccgcatt gaccacagaa
     4381 cacttgaggc gcagggcatc gagcgcctgc ccggtgttca cctggggccg aacgtggtgg
     4441 agatggaagg ccggggcatc cgcaccgacc gggcagacgt ggccctgaac atcgacaccg
     4501 ccaacgccca gatcatcgac ttacaggaat accgggaggc aatagaccat gaacgcaatc
     4561 gacagagtga agaaatccag aggcatcaac gagttagcgg agcagatcga accgctggcc
     4621 cagagcatgg cgacactggc cgacgaagcc cggcaggtca tgagccagac ccagcaggcc
     4681 agcgaggcgc aggcggcgga gtggctgaaa gcccagcgcc agacaggggc ggcatgggtg
     4741 gagctggcca aagagttgcg ggaggtagcc gccgaggtga gcagcgccgc gcagagcgcc
     4801 cggagcgcgt cgcgggggtg gcactggaag ctatggctaa ccgtgatgct ggcttccatg
     4861 atgcctacgg tggtgctgct gatcgcatcg ttgctcttgc tcgacctgac gccactgaca
     4921 accgaggacg gctcgatctg gctgcgcttg gtggcccgat gaagaacgac aggactttgc
     4981 aggccatagg ccgacagctc aaggccatgg gctgtgagcg cttcgatatc ggcgtcaggg
     5041 acgccaccac cggccagatg atgaaccggg aatggtcagc cgccgaagtg ctccagaaca
     5101 cgccatggct caagcggatg aatgcccagg gcaatgacgt gtatatcagg cccgccgagc
     5161 aggagcggca tggtctggtg ctggtggacg acctcagcga gtttgacctg gatgacatga
     5221 aagccgaggg ccgggagcct gccctggtag tggaaaccag cccgaagaac tatcaggcat
     5281 gggtcaaggt ggccgacgcc gcaggcggtg aacttcgggg gcagattgcc cggacgctgg
     5341 ccagcgagta cgacgccgac ccggccagcg ccgacagccg ccactatggc cgcttggcgg
     5401 gcttcaccaa ccgcaaggac aagcacacca cccgcgccgg ttatcagccg tgggtgctgc
     5461 tgcgtgaatc caagggcaag accgccaccg ctggcccggc gctggtgcag caggctggcc
     5521 agcagatcga gcaggcccag cggcagcagg agaaggcccg caggctggcc agcctcgaac
     5581 tgcccgagcg gcagcttagc cgccaccggc gcacggcgct ggacgagtac cgcagcgaga
     5641 tggccgggct ggtcaagcgc ttcggtgatg acctcagcaa gtgcgacttt atcgccgcgc
     5701 agaagctggc cagccggggc cgcagtgccg aggaaatcgg caaggccatg gccgaggcca
     5761 gcccagcgct ggcagagcgc aagcccggcc acgaagcgga ttacatcgag cgcaccgtca
     5821 gcaaggtcat gggtctgccc agcgtccagc ttgcgcgggc cgagctggca cgggcaccgg
     5881 caccccgcca gcgaggcatg gacaggggcg ggccagattt cagcatgtag tgcttgcgtt
     5941 ggtactcacg cctgttatac tatgagtact cacgcacaga agggggtttt atggaatacg
     6001 aaaaaagcgc ttcagggtcg gtctacctga tcaaaagtga caagggctat tggttgcccg
     6061 gtggctttgg ttatacgtca aacaaggccg aggctggccg cttttcagtc gctgatatgg
     6121 ccagccttaa ccttgacggc tgcaccttgt ccttgttccg cgaagacaag cctttcggcc
     6181 ccggcaagtt tctcggtgac tgatatgaaa gaccaaaagg acaagcagac cggcgacctg
     6241 ctggccagcc ctgacgctgt acgccaagcg cgatatgccg agcgcatgaa ggccaaaggg
     6301 atgcgtcagc gcaagttctg gctgaccgac gacgaatacg aggcgctgcg cgagtgcctg
     6361 gaagaactca gagcggcgca gggcgggggt agtgaccccg ccagcgccta accaccaact
     6421 gcctgcaaag gaggcaatca atggctaccc ataagcctat caatattctg gaggcgttcg
     6481 cagcagcgcc gccaccgctg gactacgttt tgcccaacat ggtggccggt acggtcgggg
     6541 cgctggtgtc gcccggtggt gccggtaaat ccatgctggc cctgcaactg gccgcacaga
     6601 ttgcaggcgg gccggatctg ctggaggtgg gcgaactgcc caccggcccg gtgatctacc
     6661 tgcccgccga agacccgccc accgccattc atcaccgcct gcacgccctt ggggcgcacc
     6721 tcagcgccga ggaacggcaa gccgtggctg acggcctgct gatccagccg ctgatcggca
     6781 gcctgcccaa catcatggcc ccggagtggt tcgacggcct caagcgcgcc gccgagggcc
     6841 gccgcctgat ggtgctggac acgctgcgcc ggttccacat cgaggaagaa aacgccagcg
     6901 gccccatggc ccaggtcatc ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta
     6961 tcgtgttcct gcaccatgcc agcaagggcg cggccatgat gggcgcaggc gaccagcagc
     7021 aggccagccg gggcagctcg gtactggtcg ataacatccg ctggcagtcc tacctgtcga
     7081 gcatgaccag cgccgaggcc gaggaatggg gtgtggacga cgaccagcgc cggttcttcg
     7141 tccgcttcgg tgtgagcaag gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc
     7201 ggcatgacgg cggggtgctc aagcccgccg tgctggagag gcagcgcaag agcaaggggg
     7261 tgccccgtgg tgaagcctaa gaacaagcac agcctcagcc acgtccggca cgacccggcg
     7321 cactgtctgg cccccggcct gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag
     7381 ctggacgtga cgtatgacta cggcgacggc aagcggatcg agttcagcgg cccggagccg
     7441 ctgggcgctg atgatctgcg catcctgcaa gggctggtgg ccatggctgg gcctaatggc
     7501 ctagtgcttg gcccggaacc caagaccgaa ggcggacggc agctccggct gttcctggaa
     7561 cccaagtggg aggccgtcac cgctgatgcc atggtggtca aaggtagcta tcgggcgctg
     7621 gcaaaggaaa tcggggcaga ggtcgatagt ggtggggcgc tcaagcacat acaggactgc
     7681 atcgagcgcc tttggaaggt atccatcatc gcccagaatg gccgcaagcg gcaggggttt
     7741 cggctgctgt cggagtacgc cagcgacgag gcggacgggc gcctgtacgt ggccctgaac
     7801 cccttgatcg cgcaggccgt catgggtggc ggccagcatg tgcgcatcag catggacgag
     7861 gtgcgggcgc tggacagcga aaccgcccgc ctgctgcacc agcggctgtg tggctggatc
     7921 gaccccggca aaaccggcaa ggcttccata gataccttgt gcggctatgt ctggccgtca
     7981 gaggccagtg gttcgaccat gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag
     8041 ctggtcgcgc tgggctggac ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg
     8101 cccaaggcgg caggctgacc ccccccactc tattgtaaac aagacatttt tatcttttat
     8161 attcaatggc ttattttcct gctaattggt aataccatga aaaataccat gctcagaaaa
     8221 ggcttaacaa tattttgaaa aattgcctac tgagcgctgc cgcacagctc cataggccgc
     8281 tttcctggct ttgcttccag atgtatgctc ttctgctccc gaacgccagc aagacgtagc
     8341 ccagcgcgtc ggccagcttg caattcgcgc taacttacat taattgcgtt gcgctcactg
     8401 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8461 gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg
     8521 caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct
     8581 ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga
     8641 gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga
     8701 ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt
     8761 gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca
     8821 gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc
     8881 agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg
     8941 gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat
     9001 aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca
     9061 ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact
     9121 gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc
     9181 taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac
     9241 aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg
     9301 tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc
     9361 ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac
     9421 ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt
     9481 caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgca
     9541 ccattcgatg gtgtcaacgt aaatgccgct tcgccttcgc gcgcgaattg caagctgatc
     9601 cgggcttatc gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg
     9661 tggtatggct gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc
     9721 gttctggata atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga
     9781 gc
//
