LOCUS       sequence-335273-        2489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Omega2 4xLexAOp for assembly of larger LexAOp response elements.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: p4xLexAOp-O2
FEATURES             Location/Qualifiers
     source          1..2489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(477..1065)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(557..576)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1180..1282
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1283..1942
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1349..1368)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2055..2483
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2142..2161)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2352..2373
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaatattaac gcttacaatt ttaactgcag gtctcagtca ggaggcatgc gtcgactcta
       61 gacccgggca gctgcgctgt caggagagct tgggctgcag gtcgaggcta aaaaactaat
      121 cgcattatca tcccctcgac gtactgtaca tataaccact ggttttatat acagcagtac
      181 tgtacatata accactggtt ttatatacag cagtcgacgt actgtacata taaccactgg
      241 ttttatatac agcagtactg tacatataac cactggtttt atatacagca gtcgaggtaa
      301 gattagatat ggatatgtat atggatatgt atatggtggt aatgccatgt aatatgccgc
      361 ttgagacctg cagtgacacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      421 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      481 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      541 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      601 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      661 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      721 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      781 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      841 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      901 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      961 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1021 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1081 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1141 agattatcaa aaaggatctt cacctagatc cttttgtgat gatcggcacg taagaggttc
     1201 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
     1261 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     1321 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     1381 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     1441 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     1501 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     1561 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     1621 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     1681 tttccctaaa gggtttattg agaatatgtt tttcgtgtca gccaatccct gggtgagttt
     1741 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     1801 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     1861 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     1921 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
     1981 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga tgataagctg
     2041 tcaaacatga tgttacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     2101 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     2161 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     2221 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     2281 ttcacgtagt gggccagcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     2341 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     2401 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     2461 ttaacaaaaa tttaacgcga attttaaca
//
