LOCUS       sequence-33529-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  TITLE     Gilmore Lab Plasmids
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA-FLAG-Nv-Bcl-3
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttgcgactc tcggctactg agacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccatg gactacaagg acgacgatga caagttaaca
      121 cgtgctgcag atgcgaattc catgatagaa actactttat ctcagcattt caaatgttta
      181 tcgaccagga aaattgaaac gccacgaaat attttgaacg gatcttatcg cggtgttgag
      241 gttaaaatgt cgacaagaaa ttggcgagaa aatactgtga aaaatggacg gaatataaag
      301 tgcacggcga aggacagaat acgcgagaag ctcacagccc gtgtgtcccc tatgagtgtc
      361 ggtatttgcc gaattacaga atcaatgagc tcaaatcaag ccgagaaaga aacttctggc
      421 cttgatccta aatttaaagc actggaacaa cagcagagca accctccacg gaagcacaaa
      481 tttcatacgc aagacccgga aaaaacaccg gcaaaaagag tgtgcacaag taacgaaaaa
      541 aacaattctg cagcagcatc cttagtttca acacaaggga ataaggaaag tttatcaagc
      601 acagagagca cgtcggggga tagcctagaa acactggtat ctgcgtcaca gtgtcaccta
      661 gcaacgctcc aggacgagga tggtgacaca cccctgcata tcgccatcgc ccaggaaaac
      721 aagaacttgg tgctgtacct cgtcagcctg atgcggtgtc tgactctcga catctacaac
      781 aacctcagac agactccact acacatagcg gtcatcacgg atcaaccgga catcgtcaag
      841 tgcctcattt acgcaggcgc caaccccaac ctaccggacc gcaatggaca gacgcctgca
      901 cacttggcat gccaacggtc agca
//
