LOCUS       sequence-33535-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Sullivan JC, Wolenski FS, Reitzel AM, French CE, Traylor-Knowles N, 
            Gilmore TD, Finnerty JR
  TITLE     Two alleles of NF-kappaB in the sea anemone Nematostella vectensis 
            are widely dispersed in nature and encode proteins with distinct 
            activities.
  JOURNAL   PLoS One. 2009  . 4(10):e7311.
  PUBMED    19806194
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2009  . 4(10):e7311."
COMMENT     Sequence Label: pcDNA-FLAG-Nv-NF-kB-Ser
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             89..112
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagcttg gtaccatgga ctacaaggac gacgatgaca agttaacacg
      121 tgctgcagat gcgaattcga cacagtctga acagcaagtg ggtagtgctc tcacagaagg
      181 catgttaaat gagatcattc gacctggtta tctacctgat atcagtgctc tgcatgtccc
      241 attaggcaca aatgcagagg agccttctta caccgagccc tacctagaaa ttcttgaaca
      301 gcctaaaccg cgtggtttcc gattccgata cccaagtgaa gggccttccc atgggggact
      361 ccctgggcag ttttcgactt ctaaaagcaa gtcatacccg tctgtccagg tgaacaacta
      421 ccaaggacca tgtagaatag ttgtaacctt ggtcacaaaa gatgaacctt atatgctaca
      481 tgcacatagt ttaactggga agaatgctaa tgaagaagga gtagtcaccg tacaagtggg
      541 gccggatcaa cacatgacag cgagcttccc aaacttggga atccagcatg tcactaaaaa
      601 gaatgttgtt aaagtactaa tggatagatt tataaagtgg caaactctgc aaaatgcaac
      661 tttcgccaaa ttgtcagaag gtatcaagga tggtgtagac ctatcactct ttggtgtcaa
      721 tacagccata aactcaaaca agcttgggtt tgacaaaaat gtggcgctct ctgtcgcaaa
      781 ccaagaagct gccaaaagta gagagtacgc taagcaacag gccgctgcca tggatctaag
      841 tgctgtgaga ctctgcttcc aggcataccc taccagaacc aagaatggca actttaccag
      901 a
//
