LOCUS       sequence-33541-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Herscovitch M, Comb W, Ennis T, Coleman K, Yong S, Armstead B, 
            Kalaitzidis D, Chandani S, Gilmore TD
  TITLE     Intermolecular disulfide bond formation in the NEMO dimer requires 
            Cys54 and Cys347.
  JOURNAL   Biochem Biophys Res Commun. 2008 Feb 29. 367(1):103-8.
  PUBMED    18164680
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun. 2008 Feb 29. 367(1):103-8."
COMMENT     Sequence Label: pcDNA-FLAG-NEMO-C54/347A
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..56
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        37..55
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             78..101
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
       61 ccaagcttgg taccatggac tacaaggacg acgatgacaa gttaacacgt gctgcagatg
      121 cgaattcaaa taggcacctc tggaagagcc aactgtgtga gatggtgcag cccagtggtg
      181 gcccggcagc agatcaggac gtactgggcg aagagtctcc tctggggaag ccagccatgc
      241 tgcacctgcc ttcagaacag ggcgctcctg agaccctcca gcgcgccctg gaggagaatc
      301 aagagctccg agatgccatc cggcagagca accagattct gcgggagcgc tgcgaggagc
      361 ttctgcattt ccaagccagc cagagggagg agaaggagtt cctcatgtgc aagttccagg
      421 aggccaggaa actggtggag agactcggcc tggagaagct cgatctgaag aggcagaagg
      481 agcaggctct gcgggaggtg gagcacctga agagatgcca gcagcagatg gctgaggaca
      541 aggcctctgt gaaagcccag gtgacgtcct tgctcgggga gctgcaggag agccagagtc
      601 gcttggaggc tgccactaag gaatgccagg ctctggaggg tcgggcccgg gcggccagcg
      661 agcaggcgcg gcagctggag agtgagcgcg aggcgctgca gcagcagcac agcgtgcagg
      721 tggaccagct gcgcatgcag ggccagagcg tggaggccgc gctccgcatg gagcgccagg
      781 ccgcctcgga ggagaagagg aagctggccc agttgcaggt ggcctatcac cagctcttcc
      841 aagaatacga caaccacatc aagagcagcg tggtg
//
