LOCUS       sequence-335411-        5699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express ExbB (225-234 Ala),ExbD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5699)
  TITLE     Postle Lab Plasmids
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKP2025
FEATURES             Location/Qualifiers
     source          1..5699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(1557..1574)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1727..1813
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1905..1932
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1951..2042
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2043..2111,2112..2903)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2261..2280)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2945..3400
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3032..3051)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3242..3263
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      3511..4099
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4000..4019
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4253..4270
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4436..4458
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4596..4615
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(4795..5673)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
      301 ctagcaggag gaattcacca tgggtaataa tttaatgcag acggaccttt ccgtctgggg
      361 tatgtatcag cacgccgata ttgtcgttaa gtgcgtgatg attgggctta ttttggcctc
      421 cgtagtcacc tgggcaatct tcttcagtaa gagcgtagag ttcttcaatc agaagcgtcg
      481 ccttaagcgc gagcagcaac tgctggctga agcgcgttcc ttaaaccagg ccaacgatat
      541 cgccgctgat tttggtagca aaagcttaag cctgcatttg ctcaatgaag cgcagaatga
      601 gctggaactg tcagaaggca gcgacgataa cgaaggtatt aaagaacgta ccagcttccg
      661 tctggagcgt cgggtcgccg cagtgggtcg tcaaatgggc cgcggtaacg gctatctggc
      721 aaccatcggc gcgatttctc cgttcgttgg tctgtttggt acggtctggg gcatcatgaa
      781 cagctttatt ggtatcgcgc aaacgcagac cactaacctg gcagtcgttg cgccgggtat
      841 cgcagaagct ctgttagcaa cggcaatcgg cctcgtggca gcgattcctg cggtcgttat
      901 ctataacgta tttgcacgcc agattggcgg ctttaaagcg atgctgggtg atgttgcagc
      961 gcaggtattg ttgctgcaaa gccgtgacct ggctgcagca gctgcagcag ctgcagcagc
     1021 tgttcgtgtc gcacaaaaat tacgcgcagg ataatatccg atggcaatgc atcttaacga
     1081 aaacctcgac gataacggcg aaatgcatga tatcaacgtg acgccgttta tcgacgtgat
     1141 gttggttctg ctgattatct ttatggtggc ggcaccgtta gcgacggtag atgtgaaggt
     1201 gaacttgcct gcttctacca gcacgccgca gccgcgcccg gaaaaaccgg tttatctgtc
     1261 ggtgaaggca gacaactcga tgtttatcgg taacgatccg gtcaccgatg aaacaatgat
     1321 tacggcgttg aatgcgttaa ccgaaggcaa gaaagacacc accatcttct tccgagcgga
     1381 caaaaccgtc gattacgaga cgttgatgaa ggtaatggat acgctgcatc aggcgggtta
     1441 cctgaagata ggtctggtcg gcgaagaaac cgccaaagcg aagtaagggg atcctctaga
     1501 gtcgacctgc aggcatgcaa gcttggctgt tttggcggat gagagaagat tttcagcctg
     1561 atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt
     1621 agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat
     1681 ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
     1741 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     1801 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg
     1861 gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac
     1921 ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata
     1981 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2041 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     2101 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     2161 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     2221 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     2281 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     2341 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     2401 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     2461 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     2521 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     2581 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     2641 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     2701 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     2761 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     2821 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     2881 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2941 atttacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3001 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3061 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3121 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     3181 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3241 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     3301 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3361 tttaacgcga attttaacaa aatattaacg tttacaattt aaaaggatct aggtgaagat
     3421 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3481 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3541 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3601 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     3661 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3721 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3781 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3841 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3901 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3961 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4021 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4081 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4141 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     4201 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     4261 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     4321 tatttcacac cgcatagggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     4381 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4441 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gaaggagatg
     4501 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
     4561 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
     4621 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgc
     4681 tcatgtttga cagcttatca tcgatgcata atgtgcctgt caaatggacg aagcagggat
     4741 tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta ccaattatga
     4801 caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct
     4861 ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt
     4921 gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat
     4981 acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga
     5041 cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc
     5101 tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg
     5161 gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc
     5221 cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc
     5281 gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga
     5341 cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata
     5401 ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa
     5461 aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg
     5521 aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag
     5581 ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc
     5641 cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagag
//
