LOCUS       sequence-335832-        8608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HiLITR protease with MAVS [Y536delinsFIVLI, R537A, R538A] C-terminal
            tmd targeting information (Mitochondria).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Coukos RW, Yao D, Sanchez ML, Strand ET, Olive ME, Udeshi ND, 
            Weissman JS, Carr SA, Bassik MC, Ting AY
  TITLE     An engineered transcriptional reporter of protein localization 
            identifies regulators of mitochondrial and ER membrane protein 
            trafficking in high-throughput CRISPRi screens.
  JOURNAL   eLife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142.
  PUBMED    34414886
REFERENCE   2  (bases 1 to 8608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-08-20"; volume: "10. pii: 69142. doi"; pages: " 
            10.7554/eLife.69142"
COMMENT     Sequence Label: EGFP-uTEV1-MAVS-mTA6
FEATURES             Location/Qualifiers
     source          1..8608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     177..196
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     436..457
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      499..508
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(525..839)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(528..552)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    599..617
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    635..653
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    670..688
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    706..724
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    742..760
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    777..795
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    813..831
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    893..1010
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1223..1267)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1452..1685
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2178..2303
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2350..2530
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2531..2757)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2785..2805)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2785..2803)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2824..2840)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2824..2840)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2837..2859)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2848..2864
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2872..2878,2879..2896,2897..2902))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2917..2938
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3055..3072)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3226..3814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3306..3325)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3985..4776,4777..4845))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4608..4627
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4846..4917)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4917..5052
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5092..5213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5103..5122)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5157..5176
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5285..5518
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             5723..5734
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6132..6152
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(6192..6200,6201..6236,6237..6239,6240..6275,
                     6276..6278,6279..6314,6315..6320,6321..6938))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(6948..7458)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7011..7029)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(join(8394..8608,1..496,497..499,500..502))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8394..8608,1..496,497..499,500..502))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8436..8457)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
       61 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
      121 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
      181 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
      241 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
      301 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
      361 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
      421 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
      481 gctcctcgcc cttgctcacc atggtggcgc tagccaattc tccaggcgat ctgacggttc
      541 actaaacgag ctctgcttat ataggcctcc caccgtacac gcctacctcg acatacgttc
      601 tctatcactg atagggagta aactcgacat acgttctcta tcactgatag ggataaactc
      661 gacatacgtt ctctatcact gatagggagt aaactcgaca tacgttctct atcactgata
      721 gggagtaaac tcgacatacg ttctctatca ctgataggga gtaaactcga catcgttctc
      781 tatcactgat agggagtaaa ctcgacatac gttctctatc actgataggg agtaaactcg
      841 acatatcgat tcgcggccaa agtggatctc tgctgtccct gtaataaacc cgaaaatttt
      901 gaatttttgt aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta
      961 ctattctttc ccctgcactg taccccccaa tccccccttt tcttttaaaa ttgtggatga
     1021 atactgccat ttgtctcgag gtcgagaatt gtcccctcgg ggttgggagg tgggtctgaa
     1081 acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta
     1141 ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat
     1201 ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt
     1261 cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat
     1321 tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt
     1381 tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt ccagagcaac
     1441 cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg attcttgcct
     1501 ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc
     1561 tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc tgtaccgtca
     1621 gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca aggaacaaag
     1681 ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc ttggtgggtg
     1741 ctactcctaa tggttcaatt tttactactt tatatttata taattcactt ctccaattgt
     1801 ccctcatatc tcctcctcca ggtctgaaga tcagcggccg cttgctgtgc ggtggtctta
     1861 cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt tatctctatc
     1921 ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag ttcttctgat
     1981 cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc tgatgtttct
     2041 aacaggccag gattaactgc gaatcgttct agctccctgc ttgcccatac tatatgtttt
     2101 aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg cgatctaatt
     2161 ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc tccgctagtc
     2221 aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca
     2281 agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa gtccctgttc
     2341 gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     2401 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     2461 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     2521 agagagaccc gtttatgtat cgagctaggc acttaaatac aatatctctg caatgcggca
     2581 attcagtggt tcgtccaatc catgtcagac ccgtctgttg ccttcctaat aaggcacgat
     2641 cgtaccacct tacttccacc aatcggcatg cacggtgctt tttctctcct tgtaaggcat
     2701 gttgctaact catcgttacc atgttgcaag actacaagag tattgcataa gactacatta
     2761 agcttgcagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     2821 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     2881 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     2941 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     3001 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3061 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3121 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3181 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3241 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3301 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3361 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3421 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3481 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3541 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3601 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3661 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3721 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3781 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3841 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3901 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3961 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4021 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4081 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4141 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4201 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4261 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4321 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4381 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4441 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4501 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4561 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4621 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4681 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4741 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4801 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4861 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaaaggcc
     4921 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     4981 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     5041 taggggcggg atgggcggag ttaggggcgg gatagctaga gccagacatg ataagataca
     5101 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     5161 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttcctctca
     5221 ctctctgata ttcatttctt tgcaagttat aaatactgaa taataagatg acatgaacta
     5281 ctactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     5341 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     5401 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     5461 gacccagtac aagcaaaaag cagatcttgt cttcgttggg agtgaattag cccttccagt
     5521 cccccctttt cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtctt
     5581 aaaggtacct ctagtccgga cgccgaggcg aaacaggcgg ggaggcggcc caaagggaga
     5641 tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc
     5701 cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc
     5761 gcagaatcca ggtggcaaca caggcgagca gccatggaaa ggacgtcagc ttccccgaca
     5821 acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg
     5881 cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct
     5941 gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca
     6001 cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat
     6061 acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag
     6121 cagcgtatcc acatagcgta aaaggagcaa catagttaag aatacgatat cttggatcga
     6181 tccttactta gttacccggg gagcatgtca aggtcaaaat cgtcaagagc gtcagcaggc
     6241 agcatatcaa ggtcaaagtc gtcaagggca tcggctggga gcatgtctaa gtcaaaatcg
     6301 tcaagggcgt cggtcggccc gccgctttcg cactttagct gtttctccag gccacatatg
     6361 attagttcca ggccgaaaag gaaggcaggt tcggctccct gccggtcgaa cagctcaatt
     6421 gcttgtctca gaagtggggg catagaatcg gtggtaggtg tctctctttc ctcttttgct
     6481 acttgatgct cctgttcctc caatacgcag cccagtgtaa agtggcccac ggcggacaga
     6541 gcgtacagtg cgttctccag ggagaagcct tgctgacaca ggaacgcgag ctgattttcc
     6601 agggtttcgt actgtttctc tgttgggcgg gtgccgagat gcactttagc cccgtcgcga
     6661 tgtgagagga gagcacagcg gtatgacttg gcgttgttcc gcagaaagtc ttgccatgac
     6721 tcgccttcca gggggcagaa gtgggtatga tgcctgtcca gcatctcgat tggcagggca
     6781 tcgagcaggg cccgcttgtt cttcacgtgc cagtacaggg taggctgctc aactcccagc
     6841 ttttgagcga gtttccttgt cgtcaggcct tcgataccga caccattgag taattccaga
     6901 gctccgttta tgactttgct cttgtccagt ctagacatgg tgaattgctg gggagagagg
     6961 tcggtgattc ggtcaacgag ggagccgact gccgacgtgc gctccggagg cttgcagaat
     7021 gcggaacacc gcgcgggcag gaacagggcc cacactaccg ccccacaccc cgcctcccgc
     7081 accgcccctt cccggccgct gctctcggcg cgccctgctg agcagccgct attggccaca
     7141 gcccatcgcg gtcggcgcgc tgccattgct ccctggcgct gtccgtctgc gagggtacta
     7201 gtgagacgtg cggcttccgt ttgtcacgtc cggcacgccg cgaaccgcaa ggaaccttcc
     7261 cgacttaggg gcggagcagg aagcgtcgcc ggggggccca caagggtagc ggcgaagatc
     7321 cgggtgacgc tgcgaacgga cgtgaagaat gtgcgagacc cagggtcggc gccgctgcgt
     7381 ttcccggaac cacgcccaga gcagccgcgt ccctgcgcaa acccagggct gccttggaaa
     7441 aggcgcaacc ccaaccccgg atgaattcgg ccctagtgca gagccgcccg gatcagtacg
     7501 atgaacagca ccaccaggag tgtgactacc agcacccctg tcacagccac ctggagccac
     7561 agagccccag gtgagggcct tccactacct gagccggaac tgccgctccc ctctccactc
     7621 ccaggcttac cagacccaga agtagagccg ctaccctcgc ctgatccggg cttgccagaa
     7681 cctgacgtgg aaccgaattc caccatgaaa actttatggc ccccccacaa tactgagtca
     7741 gcatttaatc gccaaccact aacccactgc tgcgcctcct gatttgtcaa caattccatg
     7801 aagtttttcg gcacgcttgt gaaataattg tttgtgttgg tgaaattcga tgctgagtgt
     7861 ataccaacaa tgaacccatc tctagttgat actaatggat tgccacactg cccatccttg
     7921 gtttgaatcc aatgcttcca gaatatgcca tcagatgaag ggaatgtgca actagtgtct
     7981 gacaccatgc tagacatgct cttagtttgg aagttggttg tcacaagaca tatgcgctct
     8041 tccctttgtg gctctctaaa tttcagcttt tgaggaaatg gtgggaaatc cttaggcatg
     8101 cgaataatta tcatgtccct cccatcaatg aggtgttgtt gcaaagtcgt ggtgttcttg
     8161 accttgaata caccatgtag tgattggacc aacagtgttc cattatttct tctaaacaag
     8221 tgcttgtttg taatgatgaa gggaccaaat ccaataccat acaacgatgt tgtgtgccca
     8281 tcagattcat tcgtcaaatg acaaatggtg ctcgatatcg ggttgtaatc acgtggtccc
     8341 ttaaacaagc tttctccgcc tgaaccggat ccgcctgtgc cagatccaag gtccttgtac
     8401 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
     8461 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
     8521 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
     8581 acgctgccgt cctcgatgtt gtggcgga
//
