LOCUS       sequence-335846-        9057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Force-activating hairpin for 3'-to-5' helicase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9057)
  AUTHORS   Taylor AMK, Okoniewski SR, Uyetake L, Perkins TT
  TITLE     Force-Activated DNA Substrates for In Situ Generation of ssDNA and 
            Designed ssDNA/dsDNA Structures in an Optical-Trapping Assay.
  JOURNAL   Methods Mol Biol. 2022;2478:273-312. doi: 
            10.1007/978-1-0716-2229-2_10.
  PUBMED    36063324
REFERENCE   2  (bases 1 to 9057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2022"; volume: "2478:273-312. doi"; pages: " 
            10.1007/978-1-0716-2229-2_10"
COMMENT     Sequence Label: hp-50%GC-20bp
FEATURES             Location/Qualifiers
     source          1..9057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..24
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(312..900)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1038..1412)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1409..1418
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1618..2412)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(1618..2412)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1729..1748)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2339..2358
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(2761..3063)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2863..2882)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     3186..3208
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3200..3217
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3201..3217
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3224..3243
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3224..3242
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3720..4046
                     /codon_start=1
                     /gene="XI"
                     /product="pXI"
                     /label=M13 gene XI
                     /note="phage assembly"
                     /translation="MKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVVSQTY
                     DFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGNSNEI
                     VKCN"
     CDS             4024..5304
                     /codon_start=1
                     /gene="IV"
                     /product="pIV"
                     /label=M13 gene IV
                     /note="phage assembly"
                     /translation="MKSLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGES
                     VIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNNQDYIDE
                     LPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKSSNV
                     LSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFAAGSQ
                     RGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRILTLSGQ
                     KGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIVLDITSK
                     ADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFLSKIPSIG
                     LLFSSRSDSNEESTLYVLVKATIVRAL"
     rep_origin      5292..5672
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5379..5398)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5589..5610
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             5909..7099
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(6261..6280)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             7738..7929
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(7923..7945)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8031..8171
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8086..8105
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8967..8983)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8967..8983)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8980..9002)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8991..9007
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9015..9021,9022..9039,9040..9045))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
       61 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      121 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      181 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      241 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      301 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      361 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      421 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      481 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      541 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      601 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      661 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      721 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
      781 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
      841 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
      901 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
      961 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1021 tgaagtttta gcacgtgtca gtcctgctcc tcggccacga agtgcacgca gttgccggcc
     1081 gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc cgatctcggt catggccggc
     1141 ccggaggcgt cccggaagtt cgtggacacg acctccgacc actcggcgta cagctcgtcc
     1201 aggccgcgca cccacaccca ggccagggtg ttgtccggca ccacctggtc ctggaccgcg
     1261 ctgatgaaca gggtcacgtc gtcccggacc acaccggcga agtcgtcctc cacgaagtcc
     1321 cgggagaacc cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc gcgcgcggtg
     1381 agcaccggaa cggcactggt caacttggcc atggtggccc tcctcacgtg ctattattga
     1441 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1501 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgatgc ggtgtgaaat
     1561 accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taataattca
     1621 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     1681 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     1741 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     1801 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
     1861 gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag
     1921 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     1981 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     2041 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     2101 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     2161 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     2221 tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     2281 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     2341 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     2401 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagagcttg atcccctgcg
     2461 ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt
     2521 accagagggc gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc
     2581 tagctatcgc catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc
     2641 ccttgtccag atagcccagt agctgacatt catccggggt cagcaccgtt tctgcggact
     2701 ggctttctac gtgaaaagga tctaggtgaa gatccttttt gataatctca tgcctgacat
     2761 ttatattccc cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag
     2821 atcagccact tcttccccga taacggagac cggcacactg gccatatcgg tggtcatcat
     2881 gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacgggaga ctttatctga
     2941 cagcagacgt gcactggcca gggggatcac catccgtcgc cccggcgtgt caataatatc
     3001 actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa
     3061 ctgccgtacg tataggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     3121 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     3181 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
     3241 gggcgaattg ggccctctag atgcatgctc gagcggccgc cagtgtgatg gatatctgca
     3301 gaattcgccc tttcaataat cggctgtctt tccttatcat tcgggcgctc ggcgtgcagc
     3361 cgtgcgggct tgttcaggac ttatctattg ttgataaaca ggcgcgttct gcattagctg
     3421 aacatgttgt ttattgtcgt cgtctggaca gaattacttt accttttgtc ggtactttat
     3481 attctcttat tactggctcg aaaatgcctc tgcctaaatt acatgttggc gttgttaaat
     3541 atggcgattc tcaattaagc cctactgttg agcgttggct ttatactggt aagaatttgt
     3601 ataacgcata tgatactaaa caggcttttt ctagtaatta tgattccggt gtttattctt
     3661 atttaacgcc ttatttatca cacggtcggt atttcaaacc attaaattta ggtcagaaga
     3721 tgaaattaac taaaatatat ttgaaaaagt tttctcgcgt tctttgtctt gcgattggat
     3781 ttgcatcagc atttacatat agttatataa cccaacctaa gccggaggtt aaaaaggtag
     3841 tctctcagac ctatgatttt gataaattca ctattgactc ttctcagcgt cttaatctaa
     3901 gctatcgcta tgttttcaag gattctaagg gaaaattaat taatagcgac gatttacaga
     3961 agcaaggtta ttcactcaca tatattgatt tatgtactgt ttccattaaa aaaggtaatt
     4021 caaatgaaat cgttaaatgt aattaatttt gttttcttga tgtttgtttc atcatcttct
     4081 tttgctcagg taattgaaat gaataattcg cctctgcgcg attttgtaac ttggtattca
     4141 aagcaatcag gcgaatccgt tattgtttct cccgatgtaa aaggtactgt tactgtatat
     4201 tcatctgacg ttaaacctga aaatctacgc aatttcttta tttctgtttt acgtgcaaat
     4261 aattttgata tggtaggttc taacccttcc attattcaga agtataatcc aaacaatcag
     4321 gattatattg atgaattgcc atcatctgat aatcaggaat atgatgataa ttccgctcct
     4381 tctggtggtt tctttgttcc gcaaaatgat aatgttactc aaacttttaa aattaataac
     4441 gttcgggcaa aggatttaat acgagttgtc gaattgtttg taaagtctaa tacttctaaa
     4501 tcctcaaatg tattatctat tgacggctct aatctattag ttgttagtgc tcctaaagat
     4561 attttagata accttcctca attcctttca actgttgatt tgccaactga ccagatattg
     4621 attgagggtt tgatatttga ggttcagcaa ggtgatgctt tagatttttc atttgctgct
     4681 ggctctcagc gtggcactgt tgcaggcggt gttaatactg accgcctcac ctctgtttta
     4741 tcttctgctg gtggttcgtt cggtattttt aatggcgatg ttttagggct atcagttcgc
     4801 gcattaaaga ctaatagcca ttcaaaaata ttgtctgtgc cacgtattct tacgctttca
     4861 ggtcagaagg gttctatctc tgttggccag aatgtccctt ttattactgg tcgtgtgact
     4921 ggtgaatctg ccaatgtaaa taatccattt cagacgattg agcgtcaaaa tgtaggtatt
     4981 tccatgagcg tttttcctgt tgcaatggct ggcggtaata ttgttctgga tattaccagc
     5041 aaggccgata gtttgagttc ttctactcag gcaagtgatg ttattactaa tcaaagaagt
     5101 attgctacaa cggttaattt gcgtgatgga cagactcttt tactcggtgg cctcactgat
     5161 tataaaaaca cttctcagga ttctggcgta ccgttcctgt ctaaaatccc ttcaatcggc
     5221 ctcctgttta gctcccgctc tgattctaac gaggaaagca cgttatacgt gctcgtcaaa
     5281 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5341 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5401 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5461 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     5521 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5581 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc
     5641 ttttgattta taagggattt tgccgatttc ggaaccacca tcaaacagga ttttcgcctg
     5701 ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agcgggcacg gccaggctcc
     5761 cgccctcagc atttgagtca acgtctggat ccatttttat ggatccagac gttgactccc
     5821 cgctgtctag aggatccgac tatcgacctc agcgctttaa tgcggtagtt tatcacagtt
     5881 aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct
     5941 cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct
     6001 cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct
     6061 atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg
     6121 ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc
     6181 gaccacaccc gtcctgtgga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     6241 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     6301 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     6361 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     6421 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc
     6481 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     6541 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     6601 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
     6661 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
     6721 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
     6781 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     6841 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     6901 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcattgg
     6961 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
     7021 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
     7081 ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca
     7141 agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag
     7201 aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt
     7261 gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg
     7321 cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac
     7381 tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt
     7441 tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc
     7501 gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat
     7561 tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca
     7621 caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct
     7681 cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg
     7741 accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg
     7801 cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt
     7861 cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa
     7921 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     7981 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     8041 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     8101 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     8161 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8221 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8281 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8341 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8401 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8461 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8521 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8581 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8641 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8701 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8761 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     8821 tgctgaagcc agttaccttc gaagggcgaa ttctgcagat atccatcaca ctggcggccg
     8881 ttactagtgg atccgagctc ggtaccaagc ttgatgcata gcttgagtat tctaacgcgt
     8941 cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     9001 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcc
//
