LOCUS       sequence-33589-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Takagi J, Strokovich K, Springer TA, Walz T
  TITLE     Structure of integrin alpha5beta1 in complex with fibronectin.
  JOURNAL   EMBO J. 2003 Sep 15. 22(18):4607-15.
  PUBMED    12970173
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2003 Sep 15. 22(18):4607-15."
COMMENT     Sequence Label: Integrin alpha5(1-623)-AHCys
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..64)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(270..281)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(345..365)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggtgaggtga gatgacggta cgcgtagaat cgagaccgag gagagggtta gggataggct
       61 taccttcgaa gggccctcta gactcgagcg gccgctgagt tccatgttcg agatagggaa
      121 actgagggcc agggagaggc agtgactcgc ccgagggcac acggcagaat gcaggcagag
      181 gcagagtggc cctgggcatc ctgagtccag ttctgggcac tctgcacctc acaggcctgg
      241 actcattgag ctagctcctt ctctagtgct tgtaattccc attccagctg tgcattttcc
      301 ttctcaagtg cctggagctc tttttcgcac tgagcatttt ctccaccttg aaaataaaga
      361 ttctcgagtc caccgttctg ctccccaaac acttccagct gcaggtcagg cacacagatg
      421 ttgtcttctc cacagtccag caagatctga gccttgtcct ctatccggct cttgctctga
      481 taatgtaggg ctggcctgag gccgtggctg tccactgggg cttgggggtc caaggagaag
      541 ttgagagcga tgtgaatcgg cgagagtttg tctcgaaatt ctgactcgtt cctgaggtag
      601 atcttcatct ctctgcaatc ctctcgagcc ccattctgga tgagcagggt ctgggtcagg
      661 gttgcctgcc tggaggccag gaacagtgcc cgccgtaccc ctcccttctg cttctgccag
      721 tccagctgaa gttccactgt gaaaccaatg gagtcagcaa cgtgttttcc agaagcattg
      781 aggcagaagc taagtga
//
