LOCUS       sequence-335909-        9270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ectopic expression of nonfluorescent BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Coukos RW, Yao D, Sanchez ML, Strand ET, Olive ME, Udeshi ND, 
            Weissman JS, Carr SA, Bassik MC, Ting AY
  TITLE     An engineered transcriptional reporter of protein localization 
            identifies regulators of mitochondrial and ER membrane protein 
            trafficking in high-throughput CRISPRi screens.
  JOURNAL   eLife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142.
  PUBMED    34414886
REFERENCE   2  (bases 1 to 9270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-08-20"; volume: "10. pii: 69142. doi"; pages: " 
            10.7554/eLife.69142"
COMMENT     Sequence Label: pLX208-nfBFP
FEATURES             Location/Qualifiers
     source          1..9270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1714..2214
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2133..2151
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2226..3020
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGAPNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2280..2299)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2890..2909
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    3062..3179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3204..4382
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3435..4373
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4330..4350
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4389..4398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5127..5715
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5180..5200)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5598..5609)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5787..6020
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6098..6219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6135..6154)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6189..6208
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6259..6394
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6414..6433)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6415..6433)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6443..6460)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6443..6459)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6452..6474)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6601..7056
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6688..6707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6898..6919
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7082..7186
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7187..7255,7256..8047)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7405..7424)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8218..8806
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8707..8726
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8960..8977
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9094..9115
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9130..9135,9136..9153,9154..9160)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9168..9184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9173..9195
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9192..9208
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9192..9208
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9227..9247
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9229..9247
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga ccattgcgcc ttttccaagg cagccctggg
     1741 tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct
     1801 gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt
     1861 gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc
     1921 gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga
     1981 cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc
     2041 tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg
     2101 gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag
     2161 cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggta
     2221 ccaccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     2281 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     2341 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccccgaatg
     2401 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     2461 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     2521 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     2581 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     2641 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     2701 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     2761 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     2821 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     2881 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     2941 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     3001 ttcttgacga gttcttctaa gaattctaga tcttgagaca aatggcagta ttcatccaca
     3061 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     3121 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     3181 gggtttatta cagggacccc gggggctccg gtgcccgtca gtgggcagag cgcacatcgc
     3241 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     3301 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     3361 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     3421 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     3481 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg
     3541 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc
     3601 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc
     3661 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac
     3721 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca
     3781 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat
     3841 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag
     3901 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg
     3961 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg
     4021 ctgcagggag ctcaaaatga aggacgcggc gctcgggaga gcgggcgggt gagtcaccca
     4081 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc
     4141 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt
     4201 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta
     4261 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt
     4321 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt
     4381 gagctagcgc caccatggtg agcaagggcg aggagctgtt taccggcgtg gtccccattc
     4441 tggtggagct ggatggcgac gtcaacggac acaaattctc cgtgagcggc gagggagaag
     4501 gagacgccac ctacggcaag ctgaccctca agttcatctg caccaccggc aaactgcccg
     4561 tgccttggcc cacactggtg accacactgt ccgctggagt gcagtgcttc tctagatacc
     4621 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgagggc tacgtccaag
     4681 agaggaccat cttcttcaag gatgacggca actacaagac aagagctgag gtgaagttcg
     4741 agggagatac cctcgtcaat agaatcgagc tgaagggcat cgacttcaag gaagacggca
     4801 acattctggg ccacaagctg gagtacaact tcaactccca taacgtgtac atcatggccg
     4861 acaagcagaa gaacggcatc aaggctaatt ttaaaattag acacaatatc gaggacggct
     4921 ccgtgcagct ggccgatcat taccagcaga atacccccat cggagacgga cccgtgctgc
     4981 tgcccgacag ccactatctg agcacacaga gcgctctgag caaggacccc aacgagaaga
     5041 gagaccacat ggtgctgctg gagtttgtga cagccgccgg catcacactg ggcatggacg
     5101 agctgtataa gtaggatatc atcggtaatc aacctctgga ttacaaaatt tgtgaaagat
     5161 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     5221 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     5281 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     5341 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     5401 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5461 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5521 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     5581 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5641 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5701 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag
     5761 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     5821 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5881 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5941 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6001 agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt
     6061 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6121 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6181 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     6241 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6301 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     6361 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     6421 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6481 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6541 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     6601 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6661 ctacacttgc cagcgccata gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6721 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6781 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6841 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6901 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6961 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7021 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     7081 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7141 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7201 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7261 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7321 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7381 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7441 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7501 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7561 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7621 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7681 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7741 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7801 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7861 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7921 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7981 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8041 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8101 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8161 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8221 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8281 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8341 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8401 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8461 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8521 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8581 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8641 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8701 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8761 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8821 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8881 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8941 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9001 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     9061 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     9121 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     9181 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     9241 ctaaagggaa caaaagctgg agctgcaagc
//
