LOCUS       sequence-335916-        8602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting 
            information (Mitochondria and ER insertion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Coukos RW, Yao D, Sanchez ML, Strand ET, Olive ME, Udeshi ND, 
            Weissman JS, Carr SA, Bassik MC, Ting AY
  TITLE     An engineered transcriptional reporter of protein localization 
            identifies regulators of mitochondrial and ER membrane protein 
            trafficking in high-throughput CRISPRi screens.
  JOURNAL   eLife. 2021 Aug 20;10. pii: 69142. doi: 10.7554/eLife.69142.
  PUBMED    34414886
REFERENCE   2  (bases 1 to 8602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-08-20"; volume: "10. pii: 69142. doi"; pages: " 
            10.7554/eLife.69142"
COMMENT     Sequence Label: EGFP-uTEV1-MAVS-mTA3
FEATURES             Location/Qualifiers
     source          1..8602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1752..1869
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1923..2237
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1931..1949
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1967..1985
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2002..2020
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2038..2056
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2074..2092
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2109..2127
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2145..2163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2210..2234
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2254..2263
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2260..2262,2263..2265,2266..2976)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2260..2262,2263..2265,2266..2976)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2305..2326)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2566..2585)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2913..2934
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        3906..4416
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4335..4353
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(4426..5043,5044..5049,5050..5085,5086..5088,5089..5124,
                     5125..5127,5128..5163,5164..5172)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5212..5232)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5630..5641)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5846..6079
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6151..6272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6188..6207)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6242..6261
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6312..6447
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6447..6518
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6519..6587,6588..7379)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6737..6756)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7550..8138
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8039..8058
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8292..8309
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8426..8447
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8462..8467,8468..8485,8486..8492)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8500..8516
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8505..8527
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8524..8540
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8524..8540
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8559..8579
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8561..8579
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga caattctcga cctcgagaca aatggcagta
     1741 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     1801 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     1861 caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg aatcgatatg
     1921 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     1981 agagaacgat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc
     2041 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
     2101 cgagtttatc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
     2161 agaacgtatg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agaattggct agcgccacca tggtgagcaa gggcgaggag
     2281 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     2341 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     2401 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     2461 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     2521 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     2581 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     2641 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     2701 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     2761 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     2821 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     2881 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     2941 gccgggatca ctctcggcat ggacgagctg tacaaggacc ttggatctgg cacaggcgga
     3001 tccggttcag gcggagaaag cttgtttaag ggaccacgtg attacaaccc gatatcgagc
     3061 accatttgtc atttgacgaa tgaatctgat gggcacacaa catcgttgta tggtattgga
     3121 tttggtccct tcatcattac aaacaagcac ttgtttagaa gaaataatgg aacactgttg
     3181 gtccaatcac tacatggtgt attcaaggtc aagaacacca cgactttgca acaacacctc
     3241 attgatggga gggacatgat aattattcgc atgcctaagg atttcccacc atttcctcaa
     3301 aagctgaaat ttagagagcc acaaagggaa gagcgcatat gtcttgtgac aaccaacttc
     3361 caaactaaga gcatgtctag catggtgtca gacactagtt gcacattccc ttcatctgat
     3421 ggcatattct ggaagcattg gattcaaacc aaggatgggc agtgtggcaa tccattagta
     3481 tcaactagag atgggttcat tgttggtata cactcagcat cgaatttcac caacacaaac
     3541 aattatttca caagcgtgcc gaaaaacttc atggaattgt tgacaaatca ggaggcgcag
     3601 cagtgggtta gtggttggcg attaaatgct gactcagtat tgtggggggg ccataaagtt
     3661 ttcatggtgg aattcggttc cacgtcaggt tctggcaagc ccggatcagg cgagggtagc
     3721 ggctctactt ctgggtctgg taagcctggg agtggagagg ggagcggcag ttccggctca
     3781 ggtagtggaa ggccctcacc tggggctctg tggctccagg tggctgtgac aggggtgctg
     3841 gtagtcacac tcctggtggt gctgttcatc gtacggcggc gtctgcacta gggccgaatt
     3901 catccggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
     3961 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
     4021 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
     4081 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
     4141 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
     4201 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
     4261 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
     4321 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
     4381 tccctcgttg accgaatcac cgacctctct ccccagcaat tcaccatgtc tagactggac
     4441 aagagcaaag tcataaacgg agctctggaa ttactcaatg gtgtcggtat cgaaggcctg
     4501 acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg
     4561 aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc
     4621 cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca
     4681 taccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca
     4741 acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc
     4801 tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta
     4861 ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct
     4921 atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc
     4981 ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc
     5041 ggcgggccga ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt
     5101 gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg
     5161 ctccccgggt aactaagtaa ggatcgatcc aagatatcgt attcttaact atgttgctcc
     5221 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5281 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5341 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5401 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5461 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5521 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     5581 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     5641 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     5701 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg tttcgcctcg
     5761 gcgtccggac tagaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     5821 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     5881 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     5941 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6001 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6061 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     6121 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     6181 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6241 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     6301 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6361 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6421 agtagtgagg aggctttttt ggaggccttt caaatatgta tccgctcatg agacaataac
     6481 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6541 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6601 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6661 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6721 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6781 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6841 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6901 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6961 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7021 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7081 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7141 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7201 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7261 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7321 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7381 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7441 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7501 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7561 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7621 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7681 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7741 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7801 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7861 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7921 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7981 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8041 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8101 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8161 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8221 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8281 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8341 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     8401 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     8461 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     8521 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     8581 aacaaaagct ggagctgcaa gc
//
