LOCUS       sequence-335921-        9074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP(UST)_EPACdDEPCD_mCherry biosensor to measure realtime changes in
            FRET reflecting fluctuations in cAMP levels in living cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Klarenbeek J, Goedhart J, van Batenburg A, Groenewald D, Jalink K
  TITLE     Fourth-generation epac-based FRET sensors for cAMP feature 
            exceptional brightness, photostability and dynamic range: 
            characterization of dedicated sensors for FLIM, for ratiometry and 
            with high affinity.
  JOURNAL   PLoS One. 2015 Apr 14;10(4):e0122513. doi: 
            10.1371/journal.pone.0122513. eCollection 2015.
  PUBMED    25875503
REFERENCE   2  (bases 1 to 9074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-14"; volume: "10(4):e0122513. doi"; pages: " 
            10.1371/journal.pone.0122513. eCollection 2015"
COMMENT     Sequence Label: Epac-S-H94
FEATURES             Location/Qualifiers
     source          1..9074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2980..3687
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3125..3143)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3393..3412
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(3739..3757)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3740..3757)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3777..3794)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3783..4007
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4053..4481
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4140..4159)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4350..4371
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4490..4510)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4495..4824
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4675..4810
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4737..4756
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4891..5685
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4945..4964)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5555..5574
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5859..5980
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5896..5915)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5950..5969
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6029..6045)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6029..6045)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6042..6064)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6053..6069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6077..6083,6084..6101,6102..6107))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6122..6143
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6260..6277)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6431..7019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6511..6530)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7190..7981,7982..8050))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7813..7832
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8051..8155)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8230..8249)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8421..8800
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8801..9004
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8955..8975
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9049..9068
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9049..9067
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aagcttgccg ccaccatggc ggccgcaatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      241 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      481 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga ctcagatctc gatatcagcc cgtgggaact
      781 catgagatgg aggaggagtt ggccgaagct gtggccctgc tctcccagcg ggggcctgac
      841 gccctgctca ctgtggcact tcgaaagccc ccaggtcagc gcacggatga agagctggac
      901 ctcatctttg aggagctgct gcacatcaag gctgtggccc acctctccaa ctcggtgaag
      961 cgagaattag cggctgttct gctctttgaa ccacacagca aggcagggac cgtgttgttc
     1021 agccaggggg acaagggcac ttcgtggtac attatctgga agggatctgt caacgtggtg
     1081 acccatggca aggggctggt gaccaccctg catgagggag atgattttgg acagctggct
     1141 ctggtgaatg atgcaccccg ggcagccacc atcatcctgc gagaagacaa ctgtcatttc
     1201 ctgcgtgtgg acaagcagga cttcaaccgt atcatcaagg atgtggaggc aaagaccatg
     1261 cggctggaag aacatggcaa agtggtgctg gtgctggaga gagcctctca gggcgccggc
     1321 ccttcccgac ccccaacccc aggcaggaac cggtatacag tgatgtctgg caccccagag
     1381 aagatcctag agcttctgtt ggaggccatg ggaccagatt ccagtgctca tgacccaaca
     1441 gagacattcc tcagcgactt cctcctgacc cacagggtct tcatgcccag cgcccaactc
     1501 tgcgctgccc ttctgcacca cttccatgtg gagcctgcgg gtggcagcga gcaggagcgc
     1561 agcacctacg tctgcaacaa gaggcagcag atcttgcggc tggtcagcca gtgggtggcc
     1621 ctgtatggct ccatgctcca cactgaccct gtggccacca gcttcctcca gaaactctca
     1681 gacctggtgg gcagggacac ccgactcagc aacctgctga gggagcagtg gccagagagg
     1741 cggcgatgcc acaggttgga gaatggctgt gggaatgcat ctcctcagat gaaggcccgg
     1801 aacttgcctg tttggctccc caaccaggac gagccccttc ctggcagcag ctgtgccatc
     1861 caagttgggg ataaagtccc ctatgacatc tgccggccag accactcagt gttgaccctg
     1921 cagctgcctg tgacagcctc cgtgagagag gtgatggcag cgttggccca ggaggatggc
     1981 tggaccaagg ggcaggtgct ggtgaaggtc aattctgcag gtgatgccat tggcctgcag
     2041 ccagatgccc gtggtgtggc cacatctctg gggctcaatg agcgtctctt tgttgtcaac
     2101 ccacaggaag cgcatgagct gatcccacac cctgaccagc tggggcccac tgtgggctct
     2161 gctgaggggc tggacctggt gagtgccaag gacctggcag gccagctgac ggaccacgac
     2221 tggagcctct tcaacagtat ccaccaggtg gagctgatcc actatgtgct gggcccccag
     2281 catctgcggg atgtcaccac cgccaacctg gagcgcttca tgcgccgctt caatgagctg
     2341 cagtactggg tggccaccga gctgtgtctc tgccccgtgc ccggcccccg ggcccagctg
     2401 ctcaggaagt tcattaagct ggcggcccac ctcaaggagc agaagaatct caattccttc
     2461 tttgccgtca tgtttggcct cagcaactcg gccatcagcc gcctagccca cacctgggag
     2521 cggctgcccc acaaagtccg gaagctgtac tccgccctcg agaggctgct ggatccctca
     2581 tggaaccacc gggtataccg actggccctc gccaagctct cccctcctgt catccccttc
     2641 atgccccttc ttctcaaaga catggccgcc attcatgagg gaaaccacac actagtggag
     2701 aatctcatca actttgagaa gatgagaatg atggccagag ccgcgcggat gctgcaccac
     2761 tgccgaagcc acaaccctgt gcctctctca ccactcagaa gccgagtttc ccacctccac
     2821 gaggacagcc aggtggcgag gatttccaca tgctcggagc agtccctgag cacccggagt
     2881 ccagccagca cctgggctta tgtccagcag ctgaaggtca ttgacaacca gcgggaactc
     2941 tcccgcctct cccgagagct ggagccagct agcgagctca tggtgagcaa gggcgaggag
     3001 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     3061 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     3121 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     3181 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     3241 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     3301 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     3361 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     3421 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     3481 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     3541 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     3601 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     3661 accggcggca tggacgagct gtacaagccg aattccctcg aggttaacgc ggcgcgcctc
     3721 taatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc
     3781 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     3841 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     3901 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     3961 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     4021 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     4081 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4141 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4201 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4261 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4321 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4381 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4441 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     4501 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4561 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4621 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4681 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4741 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     4801 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     4861 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     4921 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     4981 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     5041 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     5101 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     5161 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     5221 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     5281 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     5341 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     5401 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     5461 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     5521 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     5581 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     5641 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     5701 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     5761 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     5821 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     5881 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5941 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     6001 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6061 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6121 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6181 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6241 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6301 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6361 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6421 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6481 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6541 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6601 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6661 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6721 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6781 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6841 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     6901 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6961 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7021 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7081 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7141 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7201 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7261 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7321 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7381 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7441 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7501 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7561 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7621 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7681 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7741 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7801 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7861 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7921 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7981 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8041 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8101 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8161 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     8221 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     8281 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     8341 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     8401 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
     8461 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     8521 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     8581 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     8641 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     8701 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     8761 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     8821 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     8881 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     8941 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     9001 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
     9061 ctatagggag accc
//
